Analysis on genetic characteristics of VP1 genes of enterovirus 71 and coxsackievirus A16 in Hubei, 2014-2015

Objective To understand the genetic characteristics of Enterovirus 71(EV71) and Coxsackievirus A16(Cox A16) causing hand foot and mouth disease in Hubei province. Methods The genotype and molecular characteristics of VP1 genes of EV71 and Cox A16 strains circulating in Hubei from 2014 to 2015 were analyzed. Results The EV71 strains shared 92.3%-100.0% and 97.6%-100.0% homology respectively in nucleotide and amino acid sequences of VP1 gens. The Cox A16 strains shared 92.3%-100.0% and 96.6%-100.0% homology respectively in nucleotide and amino acid sequences of VP1 genes. Phylogenetic analysis indicated that the EV71 strains belonged to sub genotype C4a and the Cox A16 strains belonged to sub genotype B1b. Conclusion The genotypes of EV71 and Cox A16 strains in Hubei from 2014 to 2015 were C4a and B1b respectively. The strains'nucleotide and amino acid shared high homology with the strains isolated from other areas both at home and abroad.