Etiology of virus caused diarrhea in children aged ＜5 years in Wuhan, 2015

Objective To investigate the epidemiological characteristics of viral diarrhea in children under 5 years of age in Wuhan.

Methods From January to December 2015, the clinical data of the diarrhea cases and fecal specimens were collected in Wuhan Children's Hospital. Enzyme linked immunosorbent assay (ELISA) was used to detect group A rotavirus, PCR was used to detect adenovirus, the reverse transcription PCR (RT-PCR) was used to detect norovirus, sapovirus, astrovirus, group B and group C rotavirus and nested PCR was used to detect GP genotypes of rotavirus. The molecular epidemiological characteristics of viral diarrhea in children aged ＜5 years was analyzed in Wuhan, 2015.

Results The detection rate of the virus causing diarrhea showed that the main pathogen was still rotavirus, especially from September to November, rate of 50.3% in positive. The norovirus infection was higher during September-October, accounted for 40.9%. Adenovirus accounted for 2.0%, astrovirus accounted for about 1.8%, while no sapovirus, group B and group C rotavirus were detected. Viral diarrhea mainly occurred in children aged ＜2 years. Among rotavirus group A, G3P［8］ (37.4%) and G1P［8］ (17.9%) were the most common genotype.

Conclusion The pathogen spectrum of children viral diarrhea in Wuhan was diverse. Rotavirus was the major pathogen causing viral diarrhea. G3P［8］ was the most common genotypes of rotavirus group A.