ObjectiveTo analyze the epidemiological and etiological characteristics of viral diarrhea in children under five years old in Lulong county, a surveillance area of viral diarrhea, of Hebei province from 2013 to 2017 and provide evidence for the prevention and control of viral diarrhea.
MethodsA total of 1 451 stool samples were collected from diarrheal children aged ＜5 years in sentinel hospitals in Hebei province between 2013 and 2017. Rotavirus antigen was detected by using enzyme-linked immunosorbent assay (ELISA) and RV identification and genotyping were conducted with nested RT-PCR. Human caliciviruses (HuCV) and human astrovirus (HAstV) were detected by RT-PCR. Adenovirus was detected by PCR. The data was analyzed by using software SPSS 20.0.
ResultsAmong 1 451 stool samples, 968 (66.71%) were positive for at least one virus and 148 (15.29%) were positive for more than one virus. The positive rates of RV, HuCV, AV and HAstV were 46.59%, 18.88%, 8.06% and 4.14% respectively. The highest detection rates of diarrhea viruses (76.60%) and RV (56.11%) were recorded in age group 12– 17 months, followed by those in age group 18–23 months. There were significant differences in the positive rates of diarrhea viruses and RV among different age groups (
\chi _\rmtotal^2
＝52.142,
P＜0.05;
\chi _\rmRV^2
＝46.218,
P＜0.05). The detection peaks of both diarrhea viruses and RV occurred during November–March. Among the RV strains, G9 was the most common G serotype (79.29%), P［8］ was the most common P genotype (85.50%), and most strains belonged to G9P［8］ (75.30%). HuCV positive samples were mainly norovirus positive.
ConclusionRV and HuCV were the main pathogens causing infant diarrhea in Hebei during 2013–2017. Autumn and winter were the high incidence seasons.
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