ObjectiveTo explore the association between efflux pump gene Rv1456c, Rv1457c and Rv1458c expressions and phenotypic drug resistance of Mycobacterium tuberculosis.
MethodsThe RNA of 102 strains of M. tuberculosis collected through drug resistance surveillance by Ningbo Prefectural Center for Disease Control and Prevention during 2017—2018 were extracted. The gene expression levels of Rv1456c, Rv1457c and Rv1458c were detected with real-time PCR and the data were analysed by using Mann-Whitney U test.
ResultsThe medians and interquartile ranges of expressions of Rv1457c and Rv1458c in rifampicin resistant group ［0.507 (0.378–1.444), 1.842 (1.325–2.628)］, were higher than those in rifampicin sensitive group ［0.418 (0.357–0.618), 1.545 (1.189–2.065)］, the differences were significant (Z＝2.030,2.108, P＜0.05). The medians and interquartile ranges of expressions of Rv1457c and Rv1458c in multidrug resistant group ［0.538 (0.419–1.490), 1.941 (1.471–2.659)］ were higher than those in non-multidrug resistant group ［0.415 (0.337–0.618), 1.533 (1.122–2.056)］, the differences were significant (Z＝2.865,2.896, P＜0.05). There were significant difference in over-expression rates of gene Rv1456c and Rv1457c between isoniazid resistant group and isoniazid sensitive group (χ2＝4.858,6.789, P＜0.05). There was significant difference in over-expression rate of gene Rv1456c between rifampicin resistant group and rifampicin sensitive group (χ2＝8.424, P＜0.05). There was significant difference in over-expression rate of gene Rv1457c between streptomycin resistant group and streptomycin sensitive group (χ2＝4.545, P＜0.05). There were significant differences in over-expression rates of gene Rv1456c and Rv1457c between ethambutol resistant group and ethambutol sensitive group (χ2＝5.142, 10.202,P＜0.05).
ConclusionThe increases in expressions of ATP-binding cassette efflux pump gene Rv1457c and Rv1458c are associated with rifampicin resistance of M. tuberculosis.
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