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Lei Wang, Qiongcheng Chen, Lulu Hu, Yaqun Qiu, Yiman Lin, Min Jiang, Yixiang Jiang, Xiaolu Shi, Qinghua Hu, Yinghui Li. Evaluation of multiple locus variable-number tandem repeat analysis for subtyping of diarrheagenic Escherichia coli in Shenzhen[J]. Disease Surveillance, 2019, 34(10): 905-911. DOI: 10.3784/j.issn.1003-9961.2019.10.010
Citation: Lei Wang, Qiongcheng Chen, Lulu Hu, Yaqun Qiu, Yiman Lin, Min Jiang, Yixiang Jiang, Xiaolu Shi, Qinghua Hu, Yinghui Li. Evaluation of multiple locus variable-number tandem repeat analysis for subtyping of diarrheagenic Escherichia coli in Shenzhen[J]. Disease Surveillance, 2019, 34(10): 905-911. DOI: 10.3784/j.issn.1003-9961.2019.10.010
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Evaluation of multiple locus variable-number tandem repeat analysis for subtyping of diarrheagenic Escherichia coli in Shenzhen

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    • Abstract
  • ObjectiveTo explore a better subtyping method for diarrheagenic Escherichia coli (DEC) through the comparison of multiple locus variable-number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE).
    MethodsIn this study, variable-number tandem-repeat (VNTR) loci were screened for the use in MLVA for subtyping of DEC, the results were then compared with those of gold standard method-PFGE.
    ResultsAmong 58 DEC isolates, 43 PFGE patterns from three clusters (DI＝0.965) and 42 MLVA subtypes from four clusters (DI＝0.955) were obtained. The clustering of two outbreak strains showed highly consistent between the two methods.
    ConclusionAlthough there was no significant difference in discriminatory power betwee MLVA and PFGE, MLVA is a rapid, simple and high-throughput subtyping method, which are superior to PFGE and has great application value in disease surveillance and disease outbreak investigation.
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