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Zhang Jingshan, Zhao Wenxuan, Chen Meiling, Du Xiaoli, Zhao Lin, Wang Zijian, Xiao Wenjing, Zhang Weiwei, Zhao Hongqun, Li Jie, Diao Baowei, Kan Biao, Pang Bo. Comparison of the two typing methods based on the genome sequence for Vibrio parahaemolyticus[J]. Disease Surveillance, 2021, 36(5): 457-461. DOI: 10.3784/jbjc.202011050373
Citation: Zhang Jingshan, Zhao Wenxuan, Chen Meiling, Du Xiaoli, Zhao Lin, Wang Zijian, Xiao Wenjing, Zhang Weiwei, Zhao Hongqun, Li Jie, Diao Baowei, Kan Biao, Pang Bo. Comparison of the two typing methods based on the genome sequence for Vibrio parahaemolyticus[J]. Disease Surveillance, 2021, 36(5): 457-461. DOI: 10.3784/jbjc.202011050373
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Comparison of the two typing methods based on the genome sequence for Vibrio parahaemolyticus

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    • Abstract
  •   Objective  To compare the ability of the whole-genome sequencing (WGS)  single nucleotide polymorphism (SNP) typing scheme with the multilocus tandem repeat analysis (MLVA) typing scheme for molecular typing of Vibrio parahaemolyticus isolated in China, and analyze the advantages and disadvantages of the two methods.
      Methods  The MLVA scheme with six  Variable number of tandemrepeats (VNTR) loci and the SNP scheme based on the whole genome sequence were used to analyze the molecular typing of 56 strains of V. parahaemolyticus isolated in China, and evaluate the effects of these two methods with the isolates in China with quantitative indicators.
      Results  Thirty-one MLVA types were obtained from 56 strains of V. parahaemolyticus by MLVA and the D value was 0.949. Thirty-three core genome SNP types were obtained in the same collection of strains by core genome SNP typing and the D value was 0.967. The discriminatory ability of the two typing methods is very close, and the typing results are highly consistent.
      Conclusion  The 6-site MLVA typing scheme has similar typing capabilities to the typing scheme based on core genome SNP.
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