Abstract: As international transportation hubs and areas with high population density, core megacities face double challenge of the importation and subsequent local transmission of infectious diseases. Based on literature analysis, policy review and case studies, this study suggests a framework for the design of key technical toolkit for the prevention and control of imported infectious disease based on the analyses on multi-source information, including official surveillance, international open-source epidemic information, port quarantine, and urban multi-point trigger data. The toolkit consists of six modules with a modular structure and full-process closed-loop design: basic data, surveillance and early warning, risk assessment, management and intervention, risk communication and artificial intelligence tools. The design strictly complies with the Law of the People’s Republic of China on the Prevention and Treatment of Infectious Diseases and the Frontier Health and Quarantine Law of the People’s Republic of China, and other relevant laws and regulations, consider the experiences in disease prevention and control of major events, such as Beijing 2022 Winter Olympics to provide technical reference for the prevention and control of imported infectious diseases in core megacities.