Abstract: Objective To detect the minimum inhibitory concentrations (MICs) of 19 antibiotics in 10 categories to Bartonella strains, analyze the drug susceptibility of Bartonella and provide evidence for clinical antibiotic use and drug resistance monitoring. Methods The susceptibility of Bartonella strains to 19 antibiotics and the antibiotic MICs were detected by using E test, the MICs of 19 antibiotics, including doxycycline, azithromycin and rifampicin, to Bartonella were detected by using 35 strains of strains of Bartonella in 11 genera. The McFarland bacterium suspension with turbidity 2. 0 was made by using cultured Bartonella strains and coated evenly on trypsin soybean agar medium containing 5% defibrinated sheep blood. The medium was placed in incubator of 5% CO2 at 37℃ for 5-7 days to detect MICs. Results Thirty five strains were sensitive to 13 antibiotics, including doxycycline, azithromycin, erythrocin and clarithromycin, the MICs were 0.016 mg/L. Thirty four strains were sensitive to rifampicin, the MIC was 0.002 mg/L. They were not sensitive to clindamycin, amikacin, vancomycin, polymixin, and trimethoprim-sulfamethoxazole. The MICs of these antibiotics were significantly higher. Conclusion Most Bartonella strains were sensitive to 14 antibiotics, including doxycycline, but they were not sensitive to 5 antibiotics, including clindamycin. It is necessary to select antibiotics to which Bartonella is sensitive in the treatment of bartonellosis.