免疫金银染色暨相差显微镜用于钩端螺旋体病早期诊断

Early Diagnosis of Leptospirosis by Using Immuno Gold Silver Staining and Reflection Contrast Microscopy

摘要: 本研究采用免疫金银染色和相差显微镜（ＩＧＳＳ／ＲＣＭ）检测了７３份早期钩体病人血涂片。阳性９份，阳性率１２．３％。其中发病３日内的钩体病人血标本４５份，阳性率１７．８％，与血培养阳性率相当，显著高于显微镜凝集试验阳性率６．７％。结果表明，ＩＧＳＳ／ＲＣＭ是一种较为理想的钩体病早期诊断方法，较血培养和ＭＡＴ简单、快速、灵敏。如果操作仔细，还可半定量，估计到病人感染钩端螺旋体的强度。

Abstract: 73 blood samples collected from patients were detected by immuno Gold Silver Staining and Reflection Contrast Microscopy (IGSS/RCM).9 of these samples were positive by IGSS/RCM.Positive rate was 12.3%.Of 45 amples from those patients ,their onset dates were within 3 days,8 samples were positive and positive rate was 17.8% by IGSS/RCM.There was no difference between the rates by using this mothod and blood culture method,but was higher than Microscopy Agglutination Test(MAT).The authors suggested that IGSS/RCM was an ideal method for early diagnosis of Leptospirosis.In contrast with culture and MAT,it was simple,rapid and sensitive.