Gene characteristics of varicella-zoster virus
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摘要: 目的对引起上海市某小学水痘暴发的水痘-带状疱疹病毒进行基因特征分析。方法对采集到的患者疱疹液用MRC-5细胞进行病毒分离，用水痘-带状疱疹病毒标准血清鉴定病毒分离株，并通过聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)和序列测定对分离到的病毒进行基因特征分析。结果从采集的5份疱疹液标本中均分离到导致细胞病变的病毒，经水痘-带状疱疹病毒标准血清中和鉴定，证实为水痘-带状疱疹病毒；对分离病毒的ORF22、38、54、62、68等不同区段进行PCR-RFLP或序列测定结果显示：所有分离到的水痘-带状疱疹病毒均为J基因型野毒株(BglⅠ阳性，PstⅠ阳性，SmaⅠ阳性)；糖蛋白E中3B3抗原决定簇的第150位氨基酸未发现类似北美VZV-MSP株或VZV-BC株的碱基突变。结论 此次水痘暴发是由J基因型水痘-带状疱疹病毒野病毒引起。有41.1%的患者曾经接种过水痘疫苗，初免失败或者抗体水平下降可能是造成水痘突破的原因。因此有必要对病毒基因特征及水痘疫苗免疫策略进行进一步的研究。Abstract: ObjectiveTo analyze the gene characteristics of varicella-zoster virus (VZV) that caused an outbreak of chicken-pox in a primary school in Shanghai in 2007. MethodsThe virus was isolated in vesicular fluid collected from 5 patients by using MRC-5 cells and identified by standard serum for VZV. PCR-RFLP and nucleotide sequencing were performed to characterize the gene of isolated virus. ResultsThe virus that can cause cytopathic effect (CPE) of MRC-5 cells was isolated from 5 vesicular fluid samples and identified as VZV by the neutralization test with standard serum for VZV. PCR-RFLP and sequencing performed on fragment ORF22, 38, 54, 62 and 68 of VZV genome showed that all the isolated VZV were wild strains of genotype J (BglⅠ+，PstⅠ+，SmaⅠ+). VZV-MSP or VZV-BC-like genetic mutation in antigenic determinant 3B3 of glycoprotein E was not found in these VZV isolates. ConclusionThis outbreak of chicken-pox was caused by wild strain of VZV (genotype J). Among the patients, 41.1% had received varicella vaccine. Failure of initial immunization or decrease of antibody level might account for this chicken-pox outbreak. So it is necessary to conduct further study on the gene characteristics of VZV and varicella vaccine vaccination strategy.
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Key words:
- varicella-zoster virus /
- outbreak /
- immunization strategy /
- genotype J
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