临床分离艰难梭菌毒素携带特征研究

程颖 卢金星 鄢盛恺 贾红兵 李文革

程颖, 卢金星, 鄢盛恺, 贾红兵, 李文革. 临床分离艰难梭菌毒素携带特征研究[J]. 疾病监测, 2009, 24(3): 193-195. doi: 10.3784/j.issn.1003-9961.2009.03.015
引用本文: 程颖, 卢金星, 鄢盛恺, 贾红兵, 李文革. 临床分离艰难梭菌毒素携带特征研究[J]. 疾病监测, 2009, 24(3): 193-195. doi: 10.3784/j.issn.1003-9961.2009.03.015
CHENG Ying*, LU Jin-xing, YAN Sheng-kai, JIA Hong-bing, LI Wen-ge, . Study on toxin characteristics of <I>Clostridium difficile</I> isolated from hospital[J]. Disease Surveillance, 2009, 24(3): 193-195. doi: 10.3784/j.issn.1003-9961.2009.03.015
Citation: CHENG Ying*, LU Jin-xing, YAN Sheng-kai, JIA Hong-bing, LI Wen-ge, . Study on toxin characteristics of <I>Clostridium difficile</I> isolated from hospital[J]. Disease Surveillance, 2009, 24(3): 193-195. doi: 10.3784/j.issn.1003-9961.2009.03.015

临床分离艰难梭菌毒素携带特征研究

doi: 10.3784/j.issn.1003-9961.2009.03.015

Study on toxin characteristics of <I>Clostridium difficile</I> isolated from hospital

  • 摘要: 目的对医院细菌室送检粪便标本中分离出的艰难梭菌,进行毒素基因以及耐药基因的初步分析。方法将细菌室收集到的112份粪便标本,经选择性厌氧培养,谷氨酸脱氢酶(GDH)以及API 20A 生化条鉴定后,分离出12株艰难梭菌,分别采用PCR的方法检测ItcdA/I和ItcdB/I基因、二元毒素基因,克林霉素抗性基因(IermB/I)。结果12株艰难梭菌中8株为毒素基因阳性,其中ItcdA/Isup+/supItcdB/Isup+/sup为5株,占62.5%;ItcdA/Isup-/supItcdB/Isup+/sup为3株,占37.5%;二元毒素基因均为阴性;耐药基因IermB/I阳性为4株,占50%。结论艰难梭菌ItcdA/Isup-/supItcdB/Isup+/sup毒株所占比例增加,临床单独检测A毒素易造成漏检;艰难梭菌耐药现象值得关注。
  • [1]

    [1]Bartlett JG. AntibioticA-associated diarrhea[J]. <I>N Engl J Med,</I> 2002,346(5):334-339.
    [2]Poxton IR,McCoubrey J, Blair J. The pathogenicity of <I>Clostridium difficile</I> [J]. <I>Clin Microbiol Infect,</I>2001, 7: 421-427.
    [3]Vanden Berg RJ, Claas ECJ,  Oyib DH, et al. Characterization of Toxin A-Negative, Toxin B-Positive <I>Clostridium difficile</I> Isolates from Outbreaks in Different Countries by Amplified Fragment Length Polymorphism and PCR Ribotyping[J]. <I>J Clin Microbiol,</I> 2004, 42(3):1035-1041.
    [4]Popoff MR,Rubin EJ,Gill DM,et al. Actin-specific ADP-ribosyltranserase produced by a <I>Clostridium difficile</I> strain[J]. <I>Infect Immun,</I> 1988, 56:2299-2306.
    [5]Kato N, Ou CY, Kato  H, et al.Identification of Toxigenic Clostridium dijficile by the Polymerase Chain Reaction [J].<I>J Clin Microbiol,</I>1991,l(29):33-37.
    [6]Kato H, Kato N, Watanabe K, et al. Identification of Toxin A-Negative, Toxin B-Positive <I>Clostridium difficile</I> by PCR[J]. <I>Clin Microbiol,</I>1998, 36(8): 2178-2182.
    [7]Stubbs S, Rupnik M, Gibert M, et al. Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of <I>Clostridium difficile</I>[J]. <I>FEMS Microbiol Lett,</I> 2000, 186(2):307-312.
    [8]Sutcliffe J, Tait-Kamradt A, Wondrack L. Streptococcus pneumoniae and Streptococcus pyogenes resistant to macrolides but sensitive to clindamycin: a common resistance pattern mediated by an efflux system[J].<I>Antimicrob Agents Chemother,</I>1996,40(8):1817-1824.
    [9]McDonald LC, Killgore GE, Thompson A, et al. An epidemic, toxin gene–variant strain of <I>Clostridium difficile</I> [J]. <I>N Engl J Med,</I> 2005, 353(23):2433-2441.
    [10]al-Barrak A, Embil J, Dyck B, et al. An outbreak of toxin A negative, toxin B positive <I>Clostridium difficile</I>-associated diarrhea in a Canadian tertiary-care hospital[J].<I> Can Commun Dis Rep, </I>1999,25(7):65-69.
    [11]Kuijper EJ, de Weerdt J, Kato H,et al. Nosocomial outbreak of <I>Clostridium difficile</I>-associated diarrhoea due to a clindamycin-resistant enterotoxin A-negative strain[J]. <I>Eur J Clin Microbiol Infect Dis,</I>2001, 20(8):528-534.
    [12]Huang H,Wu S,Wang M,et al. <I>Clostridium difficile</I> infections in Shanghai hospital: antimicrobial resistance, toxin profiles and ribotyping [J]. <I>Int J Antimicrob Agents,</I> 2008,12: 19.
    [13]Martin H, Willey B, Low DE, et al. Characterization of <I>Clostridium difficile</I> strains isolated from patients in Ontario, Canada, from 2004 to 2006[J]. <I>J Microbiol,</I>2008,46(9): 2999-3004.

  • 加载中
计量
  • 文章访问数:  1102
  • HTML全文浏览量:  14
  • PDF下载量:  798
  • 被引次数: 0
出版历程
  • 收稿日期:  2009-02-23
  • 刊出日期:  2009-03-30

目录

    /

    返回文章
    返回

    在线交流