致泻性大肠埃希菌诱发细胞肌动蛋白聚集的研究

白莉(综述) 徐建国(指导)

引用本文: 白莉(综述), 徐建国(指导). 致泻性大肠埃希菌诱发细胞肌动蛋白聚集的研究[J]. 疾病监测, 2009, 24(4): 287-292. DOI: 10.3784/j.issn.1003-9961.2009.04.022 shu
Citation:  AI Li and XU Jian-guo. Research progress of  actin polymerization triggered by pathogenic <I>E.coli</I>[J]. Disease Surveillance, 2009, 24(4): 287-292. DOI: 10.3784/j.issn.1003-9961.2009.04.022 shu

致泻性大肠埃希菌诱发细胞肌动蛋白聚集的研究

摘要: 人类重要的肠道致病菌,肠致病性大肠埃希菌(EPEC)和肠出血性大肠埃希菌(EHEC), 在细菌黏附细胞周围形成肌动蛋白聚集的杯垫样结构来黏附并定植到肠道黏膜上,完成侵入宿主细胞的第一步。同时,被感染的肠道黏膜上皮细胞刷状缘脱落并失去微绒毛,这种特殊的病理损伤过程,称为黏附抹平效应(A/E效应)。通过细胞培养感染模型对典型菌株的研究显示,尽管EHEC和EPEC都能激活下游N-WASP蛋白,Arp2/3蛋白,最终引起有效肌动蛋白的聚集,但是它们却采取了不同的途径引起肌动蛋白的聚集。EPEC运用Tir-Nck途径,而EHEC运用是Tir-TccP途径来激活N-WASP蛋白。最近通过细胞培养感染模型研究发现,EHEC和EPEC菌株存在一种既不依赖TccP也不依赖Nck但能引起微弱肌动蛋白聚集的途径。在体内实验(in vivo)和体外组织实验(ex vivo)中,黏膜组织感染也显示不依赖TccP或Nck同样能产生典型的A/E损伤。

English

    1. [1]Staley TE, Jones EW, Corley LD. Attachment and penetration of <I>Escherichia coli</I> into intestinal epithelium of the ileum in newborn pigs[J]. <I>Am J Pathol,</I> 1996, 56 (3): 371-392.
      [2]Moon HW, Whipp SC, Argenzio  RA, et al. Attaching and effacing activities of rabbit and human enteropathogenic <I>Escherichia coli</I> in pig and rabbit intestines[J]. <I>Infect Immun,</I>1983, 41(3): 1340-1351.
      [3]Frankel G, Phillips AD, RosenshineI, et al. Enteropathogenic and enterohaemorrhagic <I>Escherichia coli</I>: more subversive elements[J]. <I>Mol Microbiol,</I>1998, 30 (5): 911-921.
      [4]Knutton S, Baldwin  T,  Williams PH, et al. Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic <I>Escherichia coli</I>[J]. <I>Infect Immun,</I>1989, 57 (4): 1290-1298.
      [5]Caron  E, Crepin VF, Simpson N, et al.  Subversion of actin dynamics by EPEC and EHEC[J]. <I>Curr Opin Microbiol,</I>2006, 9 (1): 40-45.
      [6]Campellone KG, Leong JM. Tails of two Tirs: actin pedestal formation by enteropathogenic <I>E. coli</I> and enterohemorrhagic <I>E. coli</I> O157∶H7[J].<I> Curr Opin Microbiol,</I>2003,6 (1): 82-90.
      [7]Gruenheid S, DeVinney R, Bladt F,et al.  Enteropathogenic <I>E. coli</I> Tir binds Nck to initiate actin pedestal formation in host cells[J]. <I>Nat Cell Biol,</I>2001, 3 (9): 856-859.
      [8]Elliott SJ, Yu J, Kaper JB.  The cloned locus of enterocyte effacement from enterohemorrhagic <I>Escherichia coli</I> O157∶H7 is unable to confer the attaching and effacing phenotype upon <I>E. coli</I> K-12[J]. <I>Infect Immun,</I>1999, 67 (8): 4260-4263.
      [9]Garmendia J, Phillips AD, Carlier MF, et al.  TccP is an enterohaemorrhagic <I>Escherichia coli</I> O157∶H7 type Ⅲ effector protein that couples Tir to the actin-cytoskeleton[J]. <I>Cell Microbiol,</I>2004, 6 (12): 1167-1183.
      [10]Campellone KG, Robbins D, Leong JM. EspFU is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly[J]. <I>Dev Cell, 2004,7</I> (2): 217-228.
      [11]Garmendia J, Ren Z, Tennant S, et al. Distribution of tccP in clinical enterohemorrhagic and enteropathogenic <I>Escherichia coli</I> isolates[J]. <I>J Clin Microbiol,</I>2005,43 (11): 5715-5720.
      [12]Viswanathan  VK, Koutsouris A, Lukic S, et al. Comparative analysis of EspF from enteropathogenic and enterohemorrhagic <I>Escherichia coli</I> in alteration of epithelial barrier function[J]. <I>Infect Immun,</I>2004, 72 (6): 3218-3227.
      [13]Ogura Y, Ooka T, Whale A, et al. TccP2 of O157∶H7 and non-O157 enterohemorrhagic <I>Escherichia coli</I> (EHEC): challenging the dogma of EHEC-induced actin polymerization[J]. <I>Infect Immun,</I>2007, 75 (2): 604-612.
      [14]Ooka T, Vieira  MA, Ogura Y, et al. Characterization of tccP2 carried by atypical enteropathogenic <I>Escherichia coli</I>[J]. <I>FEMS Microbiol Lett,</I>2007, 271 (1): 126-135.
      [15]Cantarelli VV, Kodama T, Nijstad N, et al. Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic <I>Escherichia coli</I> effectors: Tir and EspFu/TccP[J]. <I>Cell Microbiol,</I>2007, 9 (7): 1782-1795.
      [16]Mousnier A, Whale AD, Schuller S, et al. Cortactin recruitment by enterohemorrhagic <I>Escherichia coli</I> O157∶H7 during infection in vitro and ex vivo[J].<I> Infect Immun,</I>2008, 76 (10): 4669-4676.
      [17]DeVinney R, Puente JL, Gauthier A, et al. Enterohaemorrhagic and enteropathogenic <I>Escherichia coli</I> use a different Tir-based mechanism for pedestal formation[J].<I> Mol Microbiol,</I> 2001, 41 (6): 1445-1458.
      [18]Lommel S, Benesch S, Rohde M, et al. Enterohaemorrhagic and enteropathogenic <I>Escherichia coli</I> use different mechanisms for actin pedestal formation that converge on N-WASP[J]. <I>Cell Microbiol,</I> 2004, 6 (3): 243-254.
      [19]Campellone KG, Giese A, Tipper DJ, et al. A tyrosine-phosphorylated 12-amino-acid sequence of enteropathogenic <I>Escherichia coli</I> Tir binds the host adaptor protein Nck and is required for Nck localization to actin pedestals[J]. <I>Mol Microbiol,</I> 2002, 43 (5): 1227-1241.
      [20]Campellone KG, Brady MJ, Alamares JG, et al. Enterohaemorrhagic <I>Escherichia coli</I> Tir requires a C-terminal 12-residue peptide to initiate EspF-mediated actin assembly and harbours N-terminal sequences that influence pedestal length[J]. <I>Cell Microbiol,</I>2006,8 (9): 1488-1503.
      [21]Allen-Vercoe E, Waddell B, Toh MC, et al. Amino acid residues within enterohemorrhagic <I>Escherichia coli</I> O157∶H7 Tir involved in phosphorylation, alpha-actinin recruitment, and Nck-independent pedestal formation[J]. <I>Infect Immun,</I>2006, 74 (11): 6196-6205.
      [22]Garmendia J, Carlier MF, Egile C, et al. Characterization of TccP-mediated N-WASP activation during enterohaemorrhagic <I>Escherichia coli</I> infection[J].<I> Cell Microbio,</I>2006, 8 (9): 1444-1455.
      [23]Whale AD, Garmendia J, Gomes TA, et al. A novel category of enteropathogenic <I>Escherichia coli</I> simultaneously utilizes the Nck and TccP pathways to induce actin remodelling[J]. <I>Cell Microbiol,</I>2006, 8 (6): 999-1008.
      [24]Hayward RD, Leong JM, Koronakis V, et al. Exploiting pathogenic <I>Escherichia coli</I> to model transmembrane receptor signalling[J]. <I>Nat Rev Microbiol,</I>2006, 4 (5): 358-370.
      [25]Campellone KG, Leong JM. Nck-independent actin assembly is mediated by two phosphorylated tyrosines within enteropathogenic <I>Escherichia coli</I> Tir[J]. <I>Mol Microbiol,</I>2005, 56 (2): 416-432.
      [26]Bai L, Schuller S, Whale A, et al. Enteropathogenic <I>Escherichia coli</I> O125∶H6 triggers attaching and effacing lesions on human intestinal biopsy specimens independently of Nck and TccP/TccP2 [J]. <I>Infect Immun, </I>2008, 76 (1): 361-368.
      [27]Brady MJ, Campellone KG, Ghildiyal M, et al. Enterohaemorrhagic and enteropathogenic <I>Escherichia coli</I> Tir proteins trigger a common Nck-independent actin assembly pathway[J]. <I>Cell Microbiol,</I>2007, 9 (9): 2242-2253.
      [28]Schuller S, Chong Y, Lewin J,et al. Tir phosphorylation and Nck/N-WASP recruitment by enteropathogenic and enterohaemorrhagic <I>Escherichia coli</I> during ex vivo colonization of human intestinal mucosa is different to cell culture models[J]. <I>Cell Microbiol,</I>2007,9 (5): 1352-1364.


    1. [1]

      陈强卢珊赵爱兰熊衍文 . 大肠埃希菌(EHEC/EPEC)紧密黏附素基因的分型分析. 疾病监测, 2012, 27(2): 93-96.

    2. [2]

      刘莎金东王艺婷王怡婷杨晶周娟许彦梅白向宁濮吉兰瑞廷熊衍文徐建国 . 儿童脑膜炎大肠埃希菌毒力基因ibeC的分布研究. 疾病监测, 2015, 30(5): 376-380. DOI: 10.3784/j.issn.1003-9961.2015.05.009

    3. [3]

      陈霞赵晓菲车洁张云飞袁敏白雪梅李娟 . 527株肉鸡源大肠埃希菌的药物敏感性特征研究. 疾病监测, 2020, 35(1): 46-51. DOI: 10.3784/j.issn.1003-9961.2020.01.011

    4. [4]

      郑皓李文革杨海英吴媛车洁陈小萍卢金星 . 脓毒症常见病原菌多重实时PCR检测方法的建立. 疾病监测, 2017, 32(9): 752-756. DOI: 10.3784/j.issn.1003-9961.2017.09.012

    5. [5]

      陆红朱丽青吴庆周铁丽 . 血液感染大肠埃希菌的耐药性及其产超广谱β-内酰胺酶分析. 疾病监测, 2009, 24(1): 54-54-56. DOI: 10.3784/j.issn.1003-9961.2009.01.018

    6. [6]

      徐春泉周翠徐琦煜费静娴周武李超周明明周铁丽 . 大肠埃希菌毒力因子检测及其与耐药相关性研究. 疾病监测, 2010, 25(7): 542-546. DOI: 10.3784/j.issn.1003-9961.2010.07.011

    7. [7]

      吴莲凤陈栎江周铁丽 . 大肠埃希菌感染的临床分布及耐药特性研究. 疾病监测, 2011, 26(11): 903-905,922. DOI: 10.3784/j.issn.1003-9961.2011.11.020

    8. [8]

      张永强戎建荣李连青李浩 . 太原地区养殖场分离2株CTX-M型产ESBLs 大肠埃希菌的耐药性及耐药基因分析. 疾病监测, 2010, 25(6): 502-504. DOI: 10.3784/j.issn.1003-9961.2010.06.024

    9. [9]

      贾园春袁敏陈东科周海健陈霞赵晓菲李娟陈会波 . 临床来源碳青霉烯耐药大肠埃希菌药物敏感性及分子流行病学特征分析. 疾病监测, 2017, 32(4): 346-350. DOI: 10.3784/j.issn.1003-9961.2017.04.021

    10. [10]

      黄艳梅李德璇汪坚华鹏谭红丽 . 2013-2017年云南省某医院抗菌药物使用情况及其对大肠埃希菌和肺炎克雷伯菌的耐药性分析. 疾病监测, 2018, 33(12): 1054-1057. DOI: 10.3784/j.issn.1003-9961.2018.12.019

    11. [11]

      王红戟王冬国石庆新陈巧明 . 浙江省台州地区不同来源的大肠埃希菌耐药性分析及产超广谱-内酰胺酶、头孢菌素酶的基因分型. 疾病监测, 2012, 27(8): 616-619. DOI: 10.3784/j.issn.1003-9961.2012.8.010

    12. [12]

      郭远瑜董华丽窦琳琳徐洁颖 . 2007-2011年浙江萧山地区尿路感染患者大肠埃希菌的分离及产超广谱-内酰胺酶和耐药性分析. 疾病监测, 2012, 27(11): 873-876. DOI: 10.3784/j.issn.1003-9961.2012.11.010

    13. [13]

      刘巧突谭徽张曼禾李美兰陈金徐建国黄力保张瑾 . 从食品从业人员中分离到产志贺样毒素且具侵袭力的大肠埃希菌. 疾病监测, 1996, 11(12): 445-447. DOI: 10.3784/j.issn.1003-9961.1996.12.445

    14. [14]

      汪群水周学琴李丽民 . 从患者胆汁分离的大肠埃希菌中质粒介导的氟喹诺酮类耐药基因的检测. 疾病监测, 2015, 30(6): 493-496. DOI: 10.3784/j.issn.1003-9961.2015.06.015

    15. [15]

      徐春泉吴庆张雪青陈栎江王赛芳虞丹丹 . 大肠埃希菌产超广谱-内酰胺酶基因型及毒力因子相关性研究. 疾病监测, 2014, 29(4): 316-320. DOI: 10.3784/j.issn.1003-9961.2014.04.016

    16. [16]

      龚甫哲(摘译)龚震宇(审校) . 2017年6-7月美国亚利桑那州和犹他州农村社区与动物粪便接触有关的大肠埃希菌O157∶H7暴发疫情调查. 疾病监测, 2018, 33(10): 883-884. DOI: 10.3784/j.issn.1003-9961.2018.10.021

    17. [17]

      阳波张网郭秋生白向宁许彦梅熊衍文罗成旺 . 弥散黏附性大肠埃希菌多重PCR检测方法的建立及其在感染性腹泻患者中的流行情况. 疾病监测, 2017, 32(5): 423-427. DOI: 10.3784/j.issn.1003-9961.2017.05.017

    18. [18]

      夏文薛琳周显凤樊国印贺凤兰甘启芦 . 一起由肠侵袭性大肠埃希菌O136:K78引起的聚集性腹泻暴发. 疾病监测, 2012, 27(9): 726-728. DOI: 10.3784/j.issn.1003-9961.2012.9.018

    19. [19]

      王娉 (综述)徐建国 (审校) . 肠出血性大肠埃希菌酸抗性机制的研究进展. 疾病监测, 2010, 25(4): 324-326. DOI: 10.3784/j.issn.1003-9961.2010.04.023

    20. [20]

      孙晖刘学通赵爱兰金东熊衍文卢珊 . 肠集聚性大肠埃希菌分离株脉冲场凝胶电泳分析. 疾病监测, 2013, 28(10): 807-810. DOI: 10.3784/j.issn.1003-9961.2013.10.006

  • 加载中
计量
  • PDF下载量:  594
  • 文章访问数:  905
  • HTML全文浏览量:  33
  • 引证文献数: 0
文章相关
  • 收稿日期:  2009-01-15
  • 刊出日期:  2009-04-30
通信作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

/

返回文章

在线交流