沙门菌监测点汤卜逊沙门菌分离株的分子分型和耐药性分析

梁未丽 王洪霞 周海健 李志锋 许学斌3 李勤2 张代涛 陈春霞 于礼 闫梅英 章丽娟 冉陆 阚飙

梁未丽, 王洪霞, 周海健, 李志锋, 许学斌3, 李勤2, 张代涛, 陈春霞, 于礼, 闫梅英, 章丽娟, 冉陆, 阚飙. 沙门菌监测点汤卜逊沙门菌分离株的分子分型和耐药性分析[J]. 疾病监测, 2009, 24(6): 422-425. doi: 10.3784/j.issn.1003-9961.2009.06.014
引用本文: 梁未丽, 王洪霞, 周海健, 李志锋, 许学斌3, 李勤2, 张代涛, 陈春霞, 于礼, 闫梅英, 章丽娟, 冉陆, 阚飙. 沙门菌监测点汤卜逊沙门菌分离株的分子分型和耐药性分析[J]. 疾病监测, 2009, 24(6): 422-425. doi: 10.3784/j.issn.1003-9961.2009.06.014
sitesLIANG Wei-li*, WANG Hong-xia, ZHOU Hai-jian, LI Zhi-feng, XU Xue-bin, LI Qin, ZHANG Dai-tao, CHEN Chun-xia, YU Li, YAN Mei-ying, ZHANG Li-juan, RAN Lu, KAN Biao. Analysis on molecular typing of Salmonella thompson isolates and its drug resistance in Salmonella surveillance[J]. Disease Surveillance, 2009, 24(6): 422-425. doi: 10.3784/j.issn.1003-9961.2009.06.014
Citation: sitesLIANG Wei-li*, WANG Hong-xia, ZHOU Hai-jian, LI Zhi-feng, XU Xue-bin, LI Qin, ZHANG Dai-tao, CHEN Chun-xia, YU Li, YAN Mei-ying, ZHANG Li-juan, RAN Lu, KAN Biao. Analysis on molecular typing of Salmonella thompson isolates and its drug resistance in Salmonella surveillance[J]. Disease Surveillance, 2009, 24(6): 422-425. doi: 10.3784/j.issn.1003-9961.2009.06.014

沙门菌监测点汤卜逊沙门菌分离株的分子分型和耐药性分析

doi: 10.3784/j.issn.1003-9961.2009.06.014

Analysis on molecular typing of Salmonella thompson isolates and its drug resistance in Salmonella surveillance

  • 摘要: 目的应用分子分型技术对2007年上海和重庆市沙门菌监测点的50株汤卜逊沙门菌进行分子流行病学分析和抗生素敏感性测定,了解上海和重庆两地菌株的分子分型特征和药物敏感性特征。方法抗生素敏感性测定采用微量肉汤稀释法,分子分型方法包括脉冲场凝胶电泳(PFGE)和多位点串联重复序列分析(MLVA)。结果药敏试验显示78%的菌株存在多重耐药,其中磺胺甲恶唑和四环素的耐药率最高,其次是甲氧苄胺嘧啶;对头孢类抗生素(头孢噻肟、头孢三嗪、头孢他啶、头孢噻呋)均未产生耐药性。PFGE将50株菌分为15个带型,30株重庆分离株间有较高的相似性;MLVA分析显示除Sal16位点外,其余检测位点在所有待检菌株中没有差别。结论分子分型支持汤卜逊沙门菌引起的暴发以及散发。目前MLVA分型应用于汤卜逊沙门菌分子分型时,分型能力低于PFGE,需要进一步优化。
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出版历程
  • 收稿日期:  2009-04-15
  • 刊出日期:  2009-06-30

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