云南省玉溪市甲型副伤寒沙门菌PFGE数据库的建立及其应用

姚颖波 曾丽萍 王树坤 阚飙 刁保卫 崔志刚 吴强 杨汝松 刘红雁

姚颖波, 曾丽萍, 王树坤, 阚飙, 刁保卫, 崔志刚, 吴强, 杨汝松, 刘红雁. 云南省玉溪市甲型副伤寒沙门菌PFGE数据库的建立及其应用[J]. 疾病监测, 2009, 24(10): 770-773. doi: 10.3784/j.issn.1003-9961.2009.10.015
引用本文: 姚颖波, 曾丽萍, 王树坤, 阚飙, 刁保卫, 崔志刚, 吴强, 杨汝松, 刘红雁. 云南省玉溪市甲型副伤寒沙门菌PFGE数据库的建立及其应用[J]. 疾病监测, 2009, 24(10): 770-773. doi: 10.3784/j.issn.1003-9961.2009.10.015
YAO Ying-bo*, ZENG Li-ping, WANG Shu-shen, KAN Biao, DIAO Bao-wei, ZHAI Zhi-gang, WU Qiang, YANG Ru-song, LIU Hong-yan. *Yuxi. Establishment and application of PFGE database on <I>Salmonella enterica</I> serotype Paratyphi A in Yuxi, Yunnan[J]. Disease Surveillance, 2009, 24(10): 770-773. doi: 10.3784/j.issn.1003-9961.2009.10.015
Citation: YAO Ying-bo*, ZENG Li-ping, WANG Shu-shen, KAN Biao, DIAO Bao-wei, ZHAI Zhi-gang, WU Qiang, YANG Ru-song, LIU Hong-yan. *Yuxi. Establishment and application of PFGE database on <I>Salmonella enterica</I> serotype Paratyphi A in Yuxi, Yunnan[J]. Disease Surveillance, 2009, 24(10): 770-773. doi: 10.3784/j.issn.1003-9961.2009.10.015

云南省玉溪市甲型副伤寒沙门菌PFGE数据库的建立及其应用

doi: 10.3784/j.issn.1003-9961.2009.10.015

Establishment and application of PFGE database on <I>Salmonella enterica</I> serotype Paratyphi A in Yuxi, Yunnan

  • 摘要: 目的建立云南省玉溪市甲型副伤寒沙门菌(SPA)分离株脉冲场凝胶电泳(PFGE)数据库,分析玉溪市各分子型别SPA的流行和分布情况,为本市SPA的监测和疫情的应急处理提供科学依据。方法采用Spe Ⅰ 和 Xba Ⅰ消化细菌染色体,对来自玉溪市6县1区194株SPA分离株进行PFGE分型和聚类分析。结果194株SPA中, Xba Ⅰ带型共有7种,分别为Xba Ⅰ 01、Xba Ⅰ 02、Xba Ⅰ 06、Xba Ⅰ 07、Xba Ⅰ 09、Xba Ⅰ 10、Xba Ⅰ 19,其中Xba Ⅰ 01为优势带型,占93.81%(182/194);Spe Ⅰ带型共有10种,分别为Spe Ⅰ01~10,其中Spe Ⅰ 01、Spe Ⅰ 02为优势带型,各占46.39%(90/194) 和 43.30%(84/194)。比较分析Xba Ⅰ带型和Spe Ⅰ 带型,Spe Ⅰ 酶切比Xba Ⅰ酶切具有更多的带型,主要是Xba Ⅰ 01带型和Xba Ⅰ 02带型可以分为更多的Spe Ⅰ带型,Spe Ⅰ 01和Spe Ⅰ 02带型均来自于Xba Ⅰ 01带型。但是,也有不同的Xba Ⅰ带型归属于相同的Spe Ⅰ带型。结论建立了玉溪市SPA PFGE数据库,Spe Ⅰ酶切数据库与Xba Ⅰ酶切数据库具有较好的一致性。Spe Ⅰ 01和Spe Ⅰ 02或Xba Ⅰ 01是玉溪流行的主要克隆,同时也不容忽视其他分子型别的危害。
  • [1]

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出版历程
  • 收稿日期:  2009-08-13
  • 刊出日期:  2009-10-30

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