鼠疫耶尔森菌caf1M基因的克隆表达及其产物免疫学评价

王鹏 石国祥 程苏云 王海滨 俞东征 张建中

王鹏, 石国祥, 程苏云, 王海滨, 俞东征, 张建中. 鼠疫耶尔森菌caf1M基因的克隆表达及其产物免疫学评价[J]. 疾病监测, 2010, 25(1): 60-64. doi: 10.3784/j.issn.1003-9961.2010.01.022
引用本文: 王鹏, 石国祥, 程苏云, 王海滨, 俞东征, 张建中. 鼠疫耶尔森菌caf1M基因的克隆表达及其产物免疫学评价[J]. 疾病监测, 2010, 25(1): 60-64. doi: 10.3784/j.issn.1003-9961.2010.01.022
WANG Peng, SHI Guo-xiang, CHENG Su-yun, WANG Hai-bing, YU Dong-zheng, ZHANG Jian-zhong. Expression of caf1M gene of Yersinia pestis and evaluation of its immunological activity[J]. Disease Surveillance, 2010, 25(1): 60-64. doi: 10.3784/j.issn.1003-9961.2010.01.022
Citation: WANG Peng, SHI Guo-xiang, CHENG Su-yun, WANG Hai-bing, YU Dong-zheng, ZHANG Jian-zhong. Expression of caf1M gene of Yersinia pestis and evaluation of its immunological activity[J]. Disease Surveillance, 2010, 25(1): 60-64. doi: 10.3784/j.issn.1003-9961.2010.01.022

鼠疫耶尔森菌caf1M基因的克隆表达及其产物免疫学评价

doi: 10.3784/j.issn.1003-9961.2010.01.022
基金项目: 

国家高技术研究计划项目(2006AA2Z4A7)

详细信息
    作者简介:

    王鹏,男,山西省汾阳市人,在读博士,研究方向为鼠疫诊断技术研究

    通讯作者:

    张建中,Tel:010-61739456,Email: zhangjianzhong@icdc.cn

  • 中图分类号: 

    R516.8

Expression of caf1M gene of Yersinia pestis and evaluation of its immunological activity

Funds: 

The research was supported by grants from the Ntional Hgh Technology Research and Development Program (2006AA2Z4A7)

More Information
    Corresponding author: ZHANG Jian-zhong, Email:zhangjianzhong@icdc.cn
  • 摘要: 目的 原核表达有免疫学活性的重组caf1M蛋白(rCaf1M),并以此建立检测鼠疫抗体的辅助诊断方法。 方法 将去掉信号肽编码序列的caf1M基因片段与载体pGEX4t-1通过NcoⅠ和XhoⅠ双酶切位点进行连接,构建重组质粒caf1M-pGEX4t-1;将caf1M-pGEX4t-1转化入E.coli BL21(DE3)中诱导表达;表达产物rCaf1M经亲和层析进行纯化;以纯化rCaf1M及天然F1抗原喷制鼠疫抗体双检测NC膜,并以浙江各6份F1抗体阳性及阴性人血清标本进行应用评价。 结果 含有重组质粒caf1M-pGEX4t-1的BL21,经诱导产生了分子量约为55103的rCaf1M融合蛋白;rCaf1M融合蛋白与鼠疫菌免疫血清有较好反应;在浙江人血清标本的检测中,rCaf1M与F1抗体阳性者有明显反应,与F1抗体阴性者无反应。 结论 本研究制备的rCaf1M,与鼠疫菌免疫血清具有良好的免疫反应性,该rCaf1M可用于鼠疫辅助免疫学检测。
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出版历程
  • 收稿日期:  2009-08-28
  • 刊出日期:  2010-01-30

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