布鲁氏菌外膜蛋白的研究进展

胡剑飞 崔步云 关平原 刘志国

引用本文: 胡剑飞, 崔步云, 关平原, 刘志国. 布鲁氏菌外膜蛋白的研究进展[J]. 疾病监测, 2010, 25(5): 380-389. DOI: 10.3784/j.issn.1003-9961.2010.05.015 shu
Citation:  HU Jian-fei, CUI Bu-yun, GUAN Ping-yuan and LIU Zhi-guo. Progress in research on outer membrane proteins of Brucella[J]. Disease Surveillance, 2010, 25(5): 380-389. DOI: 10.3784/j.issn.1003-9961.2010.05.015 shu

布鲁氏菌外膜蛋白的研究进展

    作者简介: 胡剑飞,石家庄市人,硕士研究生,主要从事布鲁氏菌蛋白质组学研究;
    通信作者: 崔步云, cuibuyun@icdc.cn 关平原, cuibuyun@icdc.cn
  • 基金项目: 863课题,布鲁氏菌快速诊断技术标志性抗原研究(No. 2007AA02Z410)

摘要: 布鲁氏菌的外膜包括脂多糖、外膜蛋白和磷脂层。其中外膜蛋白是布鲁氏菌的主要免疫原和保护性抗原。根据分子质量的大小,将外膜蛋白分为3组。第一组有外膜蛋白10 k、18 k、19 k,其中Omp10、Omp16和Omp19为一种脂蛋白。第2组包括36 k~38 k外膜蛋白。第3组包括31 k~34 k外膜蛋白和25 k~27 k外膜蛋白,Omp25和Omp31与布鲁氏菌的毒力和免疫原性有密切关系。本文就这3组外膜蛋白的研究进行了总结。

English

    1. [1]. Shang DQ.Research progress on Brucellosis [J].Chinese Journal of Control of Endemic Diseases,2004,19(4):204-212.(in Chinese)尚德秋.布氏菌病研究进展[J].中国地方病防治杂志,2004,19(4):204-212.

      [2]. Hussain SA.Seroprevalence of bovine and human Brucelosis in Assam [J].Vet Bull,2001,71(7):765.

      [3]. Rajesh C. Brucellosis outbreak in an organized daifarm Involving cows and in contact human beings [J].Vet Bull,2003,73(9):1250.

      [4]. Shang DQ. Prevention and control research on Brucellosis for 50 years in China [J].Chinese Journal of Epidemiology,2000,21(1):55-57.(in Chinese)尚德秋.中国布鲁氏菌病防治科研50年[J].中华流行病学杂志,2000,21(1):55-57.

      [5]. Cui BY. Surveillance and control of Brucellosis in China [J].Disease Surveillance,2007,22(10):649-651.(in Chinese)崔步云.中国布鲁氏菌病疫情监测与控制[J].疫病监测,2007,22(10):649-651.

      [6]. Corbel MJ,Morgan BWJ. Genus BrucelLa Meyer and Shaw 1920,173AL In:Krieg, N R, Holt J(Eds), Bergey’s Manual of Systematic Bacterlology [M].Williams and Wilkins:Baltimore,1984:377-390.

      [7]. Cloeckaert A,Verger JM, Grayon M, et al. Classigication of Brucella spp.:Isolated from marine mammals by DNA polymorphism at the Omp2 locus[J]. Microbes Infect,2001,3:729-738.

      [8]. DelVecchio Vito G,Vinayak Kapatral.The genome sequence of the facultative intracellular pathogen Brucella melitensis [J]. PNAS,2002,99:443-448.

      [9]. Farshad Shohreh, Mohammad Javad Mehre Banpour. Production and characterization of monoclonal antibodies against Brucella abortus S(99)surface antigens [J]. Iran Biomed,2002,6(1):7-12.

      [10]. Cloeckaert A,Vizaino N,Paquet JY.Major outer membrane protein of Brucella spp:past,present,and future [J].Veterin Microbiol,2002,90:229-248.

      [11]. Cloeckaert A, Jacques I. Protection conferred on mice by monoclonal antibodies directed against outer-membrane-protein antigens of Brucella [J]. Med Microbiol,1991,34(3):175-180.

      [12]. Dubray, Bezard G. Isolation of three protective cell-wall antigens of Brucella abortus in experimental brucellosis in mice[J].Ann Rech Vet,1980,11:367-373.

      [13]. Dubray G, Charriaut C. Evidence of three major polypeptide and two major polysaccharide species in the Brucella outer membrane[J].Ann Rech Vet,1983,14:311-318.

      [14]. Cloeckaert A,Zygmunt MS, Wergifosse P,et al. Demonstration of peptidoglycan associated Brucella outer membrance proteins by use of monoclonal antibodies[J].Gen Microbiol,1992,138:1543-1550.

      [15]. Cloeckaert A, Kerkhofs P, Limet JN. Antibody response to Brucella outer membrane proteins in bovine brucellosis:immunoblot analysis and competitive enzyme-linked immunosorbent assay using monoclonal antibodies [J].Clin Microbiol,1992,30(12):3168-3174.

      [16]. Riezu Boj JI, Moriyón I, Blasco JM, et al. Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis[J]. Infect Immun,1990,8(2):489-494.

      [17]. Cloeckaert A, de Wergifosse P, Dubray G, et al. Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies:immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay [J]. Infect Immun,1990,58(12):3980-3987.

      [18]. Ding XZ, Bhattacharjee A, Nikolich MP, et al.Cloning, expression, and purification of Brucella suis outer membrane proteins [J]. Protein Expr Purif,2005,40(1):134-141.

      [19]. Cloeckaert A, Jacques I, Bosseray N, et al.Protection conferred on mice by monoclonal antibodies directed against outer-membrane-protein antigens of Brucella [J]. Med Microbiol,1991,34(3):175-180.

      [20]. Cloeckaert A, Kerkhofs P, Limet JK.Antibody response to Brucella outer membrane proteins in bovine brucellosis:immunoblot analysis and competitive enzyme-linked immunosorbent assay using monoclonal antibosies[J].Clin Microbil,1992,30:3168-3174.

      [21]. Zygmunt MS,Cloeckaert A,Dubray G. Brucella melitensis cell envelope protein and lipopolysaccharide epitopes involved in immune responses of naturally and experimentally infected sheep[J].Clin Microbiol,1994,32:2514-2522.

      [22]. Wagner MA, Eschenbrenner M, Horn TA, et al. Global analysis of the Brucella melitensis proteome:Identification of proteins expressed in laboratory-grown culture[J]. Proteomics,2002,2(8):1047-1060.

      [23]. Connolly JP, Comerci D, Alefantis TG, et al. Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development[J].Proteomics,2006,6(13):3767-3780.

      [24]. Wu S,Zhao ZP,Luo DY,et al.Application of immunoproteomics in research on outer membrane protein of Brucella[J].Immunological Journal,2008,24(4):385-388.(in Chinese)吴朔,赵忠鹏,罗德炎,等.布鲁氏菌外膜蛋白免疫蛋白质组学方法的建立[J].免疫学杂志,2008,24(4):385-388.

      [25]. Tibor A, Wansard V, Bielartz V, et al. Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice[J]. Infect Immun,2002,70(10):5540-5546.

      [26]. Elzer P, Phillips R, Kovach M, et al. Characterization and genetic complementation of a Brucella abortus high-temperature-requirement A(htrA)deletion mutant[J]. Infect Immun,1994,62:4135-4139.

      [27]. Zwerdling A, Delpino MV, Pasquevich KA,et al.Brucella abortus activates human neutrophils[J]. Microbes Infect,2009,11(6-7):689-697.

      [28]. Pasquevich KA, Estein SM, García Samartino C, et al. Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD+4 and CD+8T cells as well as systemic and oral protection against Brucella abortus infection[J]. Infect Immun,2009,77(1):436-445.

      [29]. Ficht TA, Bearden SW, Sowa BA, et al. DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus[J].Infect Immun,1989,57(11):3281-3291.

      [30]. Cloeckaert A, Verger JM, Grayon M, et al. Restriction site polymorphism of the genes encoding the major 25 k and 36 k outer-membrane proteins of Brucella[J]. Microbiology,1995,141:2111-2121.

      [31]. Cloeckaert A, Verger JM, Grayon M, et al.Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the Omp2 locus[J].Microbes Infect,2001,3(9):729-738.

      [32]. Ohishi K, Takishita K, Kawato M, et al. Chimeric structure of Omp2 of Brucella from Pacific common minke whales(Balaenoptera acutorostrata)[J]. Microbiol Immunol,2005,49(8):789-793.

      [33]. Burgess AW,Paquet JY,Leterson BE. Isolation,sequencing and expression of Bartonella henselae omp43 and predicted membrane topology of the deduced protein[J].Microb.Pathog,2000,29:73-80.

      [34]. Ficht TA, Bearden SW, Sowa BA, et al. DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus[J].Infect Immun,1989,57(11):3281-3291.

      [35]. Imed Salhi, Rose Anne Boigegrain, Jan Machold,et al.Characterization of New Members of the Group 3 Outer Membrane Protein Family of Brucella spp[J].Infect Immun,2003,71(8):4326-4433.

      [36]. Jubier-Maurin V, Boigegrain RA, Cloeckaert A, et al. Major outer membrane protein Omp25 of Brucella suis is involved in inhibition of tumor necrosis factor alpha production during infection of human macrophages[J].Infect Immun,2001,69(8):4823-4830.

      [37]. Boigegrain RA, Salhi I, Alvarez-Martinez MT, et al. Release of periplasmic proteins of Brucella suis upon acidic shock involves the outer membrane protein Omp25[J]. Infect Immun,2004,72(10):5693-5703.

      [38]. Wang Y, Chen Z, Qiao F, et al.The type IV secretion system affects the expression of Omp25/Omp31 and the outer membrane properties of Brucella melitensis[J]. FEMS Microbiol Lett,2009,23(11):121-125.

      [39]. Bowden RA, Cloeckaert A, Zygmunt MS, et al. Evaluation of immunogenicity and protective activity in BALB/c mice of the 25-kDa major outer-membrane protein of Brucella melitensis(Omp25)expressed in Escherichia coli[J]. Med Microbiol,1998,47(1):39-48.

      [40]. Edmonds MD, Cloeckaert A, Elzer PH. Brucella species lacking the major outer membrane protein Omp25 are attenuated in mice and protect against Brucella melitensis and Brucella ovis[J]. Vet Microbiol,2002,88(3):205-221.

      [41]. Lorea Manterola, Caterina Guzmán Verri, Esteban Chaves Olarte,et al. BvrR/BvrS-Controlled Outer Membrane Proteins Omp3a and Omp3b Are Not Essential for Brucella abortus Virulence[J]. Infect Immun,2007,75(10):4867-4874.

      [42]. Chaudhuri P, Kumar SV, Prasad R, et al. Cloning and sequencing of 28 kDa outer membrane protein gene of Brucella melitensis Rev. 1[J]. Indian Exp Biol,2005,43(9):838-840.

      [43]. Thavaselvam D, Kumar A, Tiwari S, et al. Cloning and expression of immunoreactive Brucella melitensis 28 kDa outer membrane protein(Omp28)gene and evaluation of its potential for clinical diagnosis of brucellosis[J]. Med Microbiol,2010,14(9):75-83.

      [44]. Chaudhuri P, Prasad R, Kumar V, et al. Recombinant OMP28 antigen-based indirect ELISA for serodiagnosis of bovine brucellosis[J].Mol Cell Probes,2009,16(12):195-201.

      [45]. Kaushik P, Singh DK, Kumar SV, et al. Protection of mice against Brucella abortus 544 challenge by vaccination with recombinant OMP28 adjuvanted with CpG oligonucleotides[J]. Vet Res Commun,2009,33(9):200.

      [46]. Vizcaíno N, Cloeckaert A, Zygmunt MS, et al. Cloning, nucleotide sequence, and expression of the Brucella melitensis Omp31 gene coding for an immunogenic major outer membrane protein[J]. Infect Immun,1996,64(9):3744-3751.

      [47]. Estein SM, Cassataro J, Vizcaíno N, et al. The recombinant Omp31 from Brucella melitensis alone or associated with rough lipopolysaccharide induces protection against Brucella ovis infection in BALB/c mice[J].Microbes Infect,2003,5(2):85-93.

      [48]. Vizcaino, Verger N, Grayon M, et al.DNA polymorphism at the Omp31 locus of Bricella spp. evidence for a large in Bricella abortus,and other species-species markers[J]. Microbiology,1997,143:2913-2921.

      [49]. Baily GG,Krahn JB,Drasar BS, et al. Detection of Brucella melitensis and Brucella abortus by DNA amplification[J].J Trop Med Hyg,1992,95:271-275.

      [50]. Delpino MV, Cassataro J, Fossati CA, et al. Brucella outer membrane protein Omp31 is a haemin-binding protein[J]. Microbes Infect,2006,8(5):1203-1211.

      [51]. Cassataro J, Pasquevich K, Bruno L, et al. Antibody reactivity to Omp31 from Brucella melitensis in human and animal infections by smooth and rough Brucellae[J]. Clin Diagn Lab Immunol,2004,11(1):111-115.

      [52]. Vizcaíno N, Kittelberger R, Cloeckaert A, et al.Minor nucleotide substitutions in the Omp31 gene of Brucella ovis result in antigenic differences in the major outer membrane protein that it encodes compared to those of the other Brucella species[J]. Infect Immun,2001,69(11):7020-7028.

      [53]. Cassataro J, Estein SM, Pasquevich KA, et al.Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD+4T helper 1 response that protects against Brucella melitensis infection[J]. Infect Immun,2005,73(12):8079-8088.

      [54]. Chen Y, Luo LM, Li M. Cloning, expression and immunological characterization of Brucella outer membrane protein Omp31[J]. Journal of Tropical Medicine,2006,6(2):120-124.(in Chinese)陈瑶,骆利敏,李明.布鲁氏菌外膜蛋白Omp31基因的克隆、表达和免疫学特性的初步研究[J].热带医学杂志,2006,6(2):120-124.

      [55]. Si R,Bai WT, Zhang FL, et al.Construction and expression of genes encoding BCSP31, OMP31 and L7/L12 proteins of Brucella melitensis in prokaryotic cell[J].Chinese Journal of Zoonoses,2005,21(21):961-964.(in Chinese)司瑞,白文涛,张芳琳,等.羊布鲁氏菌BCSP31、OMP31、L7/L12基因的原核表达载体构建及表达[J].中国人兽共患病杂志,2005,21(21):961-964.

      [56]. Cassataro J, Velikovsky CA, Bruno L, et al. Improved immunogenicity of a vaccination regimen combining a DNA vaccine encoding Brucella melitensis outer membrane protein 31(Omp31)and recombinant Omp31 boosting[J]. Clin Vaccine Immunol,2007,14(7):869-874.

      [57]. Estein SM, Fiorentino MA, Paolicchi FA, et al. The polymeric antigen BLSOmp31 confers protection against Brucella ovis infection in rams[J]. Vaccine,2009,27(48):6704-6711.

      [58]. Grilló MJ, Marín CM, Barberán M, et al. Efficacy of bp26 and bp26/Omp31 B.melitensis Rev.1 deletion mutants against Brucella ovis in rams[J]. Vaccine,2009,27(2):187-191.

      [59]. Zhang HX, Chen CF, Wang YZ,et al. Cloning and prokaryotic expression of Omp31 gene from Brucella M5-90[J]. Journal of Shihezi University,2009,27(5):593-597.(in Chinese)张红星,陈创夫,王远志,等.羊种布鲁氏茵M5-90 Omp31蛋白原核表达[J].石河子大学学报,2009,27(5):593-597.

      [60]. Cassataro J,Velikovsky CA,de la Barrera S,et al. DNA vaccine coding for the Brucella outer membrane protein 31 confers protection against B. melitensis and B. ovis infection by eliciting a specific cytotoxic response[J]. Infect Immun,2005,73(10):6537-6546.

      [61]. Gupta VK, Rout PK, Vihan VS.Induction of immune response in mice with a DNA vaccine encoding outer membrane protein(Omp31)of Brucella melitensis 16M[J]. Res Vet Sci,2007,82(3):305-313.

      [62]. Caro-Hernández P, Fernández-Lago L, Miguel MJ, et al. Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis[J]. Infect Immun,2007,75(8):4050-4061.

      [63]. Martín -Martín AI, Caro Hernández P, Sancho P,et al.Analysis of the occurrence and distribution of the Omp25/Omp31 family of surface proteins in the six classical Brucella species[J].Vet Microbiol,2009,137(1-2):74-82.


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