应用PCR方法快速检测布鲁氏菌核酸的研究进展

刘志国 张利 崔步云 侯勇跃 胡剑飞

引用本文: 刘志国, 张利, 崔步云, 侯勇跃, 胡剑飞. 应用PCR方法快速检测布鲁氏菌核酸的研究进展[J]. 疾病监测, 2010, 25(9): 746-751. DOI: 10.3784/j.issn.1003-9961.2010.09.025 shu
Citation:  LIU Zhi-guo, ZHANG Li, CUI Bu-yun, HOU Yong-yue and HU Jian-fei. Research advancement of rapid detection of Brucella nucleic acid using PCR[J]. Disease Surveillance, 2010, 25(9): 746-751. DOI: 10.3784/j.issn.1003-9961.2010.09.025 shu

应用PCR方法快速检测布鲁氏菌核酸的研究进展

    作者简介: 刘志国,男,内蒙古自治区集宁市人,硕士研究生,主要从事布鲁氏菌分子生物学研究工作;
    通信作者: 崔步云, cuibuyun@icdc.cn 侯勇跃, cuibuyun@icdc.cn
  • 基金项目: 863课题(No.2007AA02Z410),重大专项(2008ZX10004-007)

摘要: 本文主要就近年应用PCR方法快速诊断人畜布鲁氏菌病(布病)的进展情况进行阐述,包括用各种布鲁氏菌基因保守序列设计引物的常规PCR、不同类型检测样本的常规PCR方法对布病的快速诊断的评述;荧光定量PCR的原理、方法;各种荧光定量PCR方法在布病快速诊断中的应用及其未来应用前景的展望。

English


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