新疆结核分枝杆菌临床分离株Spoligotyping基因型的初步研究

綦迎成 刘洁 鱼栓民 李君莲 赵秀芹 王泉 刘志广 吕冰 刘中华 万康林

綦迎成, 刘洁, 鱼栓民, 李君莲, 赵秀芹, 王泉, 刘志广, 吕冰, 刘中华, 万康林. 新疆结核分枝杆菌临床分离株Spoligotyping基因型的初步研究[J]. 疾病监测, 2010, 25(12): 951-954. doi: 10.3784/j.issn.1003-9961.2010.12.007
引用本文: 綦迎成, 刘洁, 鱼栓民, 李君莲, 赵秀芹, 王泉, 刘志广, 吕冰, 刘中华, 万康林. 新疆结核分枝杆菌临床分离株Spoligotyping基因型的初步研究[J]. 疾病监测, 2010, 25(12): 951-954. doi: 10.3784/j.issn.1003-9961.2010.12.007
QI Ying-cheng, LIU Jie, YU Shuan-min, LI Jun-lian, ZHAO Xiu-qin, WANG Quan, LIU Zhi-guang, LV Bing, LIU Zhong-hua, WAN Kang-lin. Spoligotyping of clinical isolates of Mycobacterium tuberculosis in Xinjiang[J]. Disease Surveillance, 2010, 25(12): 951-954. doi: 10.3784/j.issn.1003-9961.2010.12.007
Citation: QI Ying-cheng, LIU Jie, YU Shuan-min, LI Jun-lian, ZHAO Xiu-qin, WANG Quan, LIU Zhi-guang, LV Bing, LIU Zhong-hua, WAN Kang-lin. Spoligotyping of clinical isolates of Mycobacterium tuberculosis in Xinjiang[J]. Disease Surveillance, 2010, 25(12): 951-954. doi: 10.3784/j.issn.1003-9961.2010.12.007

新疆结核分枝杆菌临床分离株Spoligotyping基因型的初步研究

doi: 10.3784/j.issn.1003-9961.2010.12.007
基金项目: 

国家自然科学基金"中国结核分枝杆菌基因多态性及株水平鉴定技术研究"(No. 30771853);国家"十一五"重大传染病防治科技重大专项"结核病传播模式研究结核病分子流行病学研究"(No. 2008ZX100/03-010-02)

详细信息
    作者简介:

    綦迎成,男,新疆自治区乌鲁木齐市人,主要从事卫生管理工作 本文1和2单位为同等贡献

    通讯作者:

    万康林, Tel:010-58900776, Email:wankanglin@icdc.cn

  • 中图分类号: 

    R338.2

Spoligotyping of clinical isolates of Mycobacterium tuberculosis in Xinjiang

  • 摘要: 目的 研究新疆结核分枝杆菌Spoligotyping基因分型,初步了解其基因型多态性状况及主要流行株。 方法 在新疆维吾尔自治区胸科医院收集一个连续时间段的结核分枝杆菌临床分离菌株,采用比例法检测进行耐药性检测、间隔寡核苷酸分型(Spoligotyping)方法进行分型研究。基因聚类分析采用BioNumerics 5.0数据库软件,统计学分析采用2检验,P0.05为差异有统计学意义。 结果 共收集到结核分枝杆菌临床分离菌株175株,其中对利福平、异烟肼、链霉素和乙胺丁醇全敏感115株,耐药60株(包括单耐药31株和耐多药29株)。经Spoligotyping分型,这些菌株可分为4个基因群49种基因型,最大的1个基因群为北京家族,占68.57%(120/175)。北京家族中,敏感菌株63.87%(76/119),耐药菌株占36.13%(43/119);非北京家族中,敏感菌株68.52%(37/54),耐药菌株占31.48%(17/54),北京家族与非北京家族的耐药率之间差异无统计学意义(P=0.551)。 结论 新疆结核分枝杆菌临床菌株存在明显的基因多态性,主要流行菌株为北京基因型。北京基因型与耐药性无明显相关性。
  • [1]


    [1] Embden JDAV, Cave MD, Crawford JT, et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: Recommendations for a standardized methodology
    [J]. J Clin Micro,1993,31:406-409.

    [2] Liebana E, Aranaz A, Francis B, et al. Assesment of genetic markers for species differentiation within the Mycobacterium tuberculosis complex
    [J]. J Clin Micro,1996,34:933-938.

    [3] Huh YJ, Ahn DI, Kim SJ. Limited variation of DNA fingerprints (IS6110 and IS1081) in Korean strains of Mycobacterium tuberculosis
    [J]. Tuber Lung Dis,1995,76:324-329.

    [4] Van Soolingen D. Molecular epidemiology of tuberculosis and other mycobacterial infections:main methodologies and achievements
    [J].J Internal Med,2001,249:1226.

    [5] Sola C, Filliol I,Legrand E,et al. Genotyping of the Mycobacterium tuberculosis complex using MIRUs association with VNTR and spoligotyping for molccular epidemiology and evolutionary genetics
    [J].Infect Genet Evol,2003,3:125-133.

    [6] Kamerbeek J, Schouls L, Kolk A, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology
    [J]. J Clin Microbiol,1997,35(4):907-914.

    [7] Kruuner A, Hoffner SE, Sillastu H, et al. Spread of drugresistant pulmonary tuberculosis in Estonia
    [J]. J Clin Microbiol,2001,39:3339-3345.

    [8] Laserson KF, Osorio L, Sheppard JD, et al. Clinical and programmatic mismanagement rather than community outbreak as the cause of chronic, drugresistant tuberculosis in Buenaventura, Colombia,1998
    [J]. Int J Tuberc Lung Dis,2000,4:673-683.

    [9] Chan MY, Borgdorff M, Yip CW, et al. Seventy percent of the Mycobacterium tubercuosis isolates in Hong Kong represent the Beijing genotype
    [J]. Epidemiol Infect,2001,127:169-171.

    [10] Glynn JR, Whiteley J, Bifani PJ, et al. Worldwide occurrence of Beijing/W strain of Mycobacterium tuberculosis: a systematic review
    [J]. Emerg Infect Dis,2002,8:843-849.

    [11] van Crevel R, Nelwan RHH, de Lenne W, et al. Mycobacterium tuberculosis Beijing genotype strains associated with febrile response to treatment
    [J]. Emerg Infect Dis,2001,7:1-4.

  • 加载中
计量
  • 文章访问数:  983
  • HTML全文浏览量:  18
  • PDF下载量:  387
  • 被引次数: 0
出版历程
  • 收稿日期:  2010-09-13
  • 刊出日期:  2010-12-30

目录

    /

    返回文章
    返回

    在线交流