海南省澄迈县新发斑点热流行病学调查

金玉明 张丽娟 孙莲英 老世军 寒江 曾昭长 朱建华 黄芳 贾鹏本 苏新元 吴维学

金玉明, 张丽娟, 孙莲英, 老世军, 寒江, 曾昭长, 朱建华, 黄芳, 贾鹏本, 苏新元, 吴维学, . 海南省澄迈县新发斑点热流行病学调查[J]. 疾病监测, 2011, 26(1): 18-22. doi: 10.3784/j.issn.1003-9961.2011.01.007
引用本文: 金玉明, 张丽娟, 孙莲英, 老世军, 寒江, 曾昭长, 朱建华, 黄芳, 贾鹏本, 苏新元, 吴维学, . 海南省澄迈县新发斑点热流行病学调查[J]. 疾病监测, 2011, 26(1): 18-22. doi: 10.3784/j.issn.1003-9961.2011.01.007
JIN Yu-ming, ZHANG Li-juan, SUN Lian-ying, LAO Shi-jun, HAN Jiang, HUANG Chang-he, WANG Shi-wen, ZENG Zhao-chang, WANG Yuan-yuan, ZHU Jian-hua, HUANG Fang, JIA Peng-ben, SU Xin-yuan, WU Wei-xue. Epidemiologyical investigation of emerging spotted fever in Chengmai county, Hainan province[J]. Disease Surveillance, 2011, 26(1): 18-22. doi: 10.3784/j.issn.1003-9961.2011.01.007
Citation: JIN Yu-ming, ZHANG Li-juan, SUN Lian-ying, LAO Shi-jun, HAN Jiang, HUANG Chang-he, WANG Shi-wen, ZENG Zhao-chang, WANG Yuan-yuan, ZHU Jian-hua, HUANG Fang, JIA Peng-ben, SU Xin-yuan, WU Wei-xue. Epidemiologyical investigation of emerging spotted fever in Chengmai county, Hainan province[J]. Disease Surveillance, 2011, 26(1): 18-22. doi: 10.3784/j.issn.1003-9961.2011.01.007

海南省澄迈县新发斑点热流行病学调查

doi: 10.3784/j.issn.1003-9961.2011.01.007
基金项目: 

国家基础研究项目-973计划(No.2010CB530200, 2010CB530206); 国家传染病防治科技重大专项(No.2008ZX10004-008, 2009ZX10004-203)

详细信息
    作者简介:

    金玉明,男,湖北省人,主要从事传染病预防控制工作. 金玉明,张丽娟为同等贡献,同为第一作者

    金玉明,男,湖北省人,主要从事传染病预防控制工作. 金玉明,张丽娟为同等贡献,同为第一作者

    通讯作者:

    张丽娟,Tel:010-58900780,Email:zhanglijuan@icdc.cn

  • 中图分类号: 

    R376

Epidemiologyical investigation of emerging spotted fever in Chengmai county, Hainan province

  • 摘要: 目的 对海南省澄迈县进行斑点热自然疫源地现场调查。 方法 以病原学证实的首发患儿居住村外延收集可疑病例并采集当地野生啮齿动物抗凝血,用现代Shell vial培养技术进行立克次体病原分离,对分离株进行 16S rRNA、gltA、ompA、17 kD蛋白基因及groEL基因扩增并测序分析。以WHO立克次体协作中心推荐间接免疫荧光试验检测患者、全县10村812名农业人群、18只家犬及44只野生啮齿动物血清斑点热群立克次体抗体。巢式PCR扩增患者、家犬、野生啮齿动物、媒介蜱、螨及蚤标本groEL基因并测序分析。分析斑点热群立克次体优势流行株及可能的媒介及宿主种类以及人群及家畜对当地斑点热群立克次体优势流行株抗体阳性率。 结果 从调查的21例可疑立克次体患者血液标本中分离到11株斑点热群立克次体,从15只野生针毛鼠血液标本中分离到7株斑点热群立克次体,遗传学分析证实分离株与黑龙江立克次体及广东野鼠斑点热群立克次体分离株关系密切,但血清表型不同黑龙江立克次体。犬血、媒介蜱、螨及蚤PCR扩增groEL基因及测序结果支持病原学证据并且序列分析结果显示与病原分离株为同一流行株。血清学结果提示当地人群对该流行株IgG抗体总阳性率为46.1%,家犬88.9%,鼠11.4%。发热可疑患者强力霉素经验治疗痊愈。 结论 正常人群、发热患者、媒介及宿主等病原学、分子生物学及血清学调查结果证实海南省存在新发斑点热疫源地,加强临床诊断及鉴别诊断,进一步监测其他蜱传立克次体病,消杀农户家居及家畜体表媒介蜱,防止叮咬是当地防治该病的关键。
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出版历程
  • 收稿日期:  2010-12-02
  • 刊出日期:  2011-01-30

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