甲型副伤寒沙门菌疫苗靶标蛋白筛选技术及应用

高翔 闫梅英 阚飙

引用本文: 高翔, 闫梅英, 阚飙. 甲型副伤寒沙门菌疫苗靶标蛋白筛选技术及应用[J]. 疾病监测, 2011, 26(7): 568-574. DOI: 10.3784/j.issn.1003-9961.2011.07.020 shu
Citation:  GAO Xiang, YAN Mei-ying and KAN Biao. Technique and its application for identifying vaccine target proteins of Salmonella paratyphi A[J]. Disease Surveillance, 2011, 26(7): 568-574. DOI: 10.3784/j.issn.1003-9961.2011.07.020 shu

甲型副伤寒沙门菌疫苗靶标蛋白筛选技术及应用

    作者简介: 高翔,女,辽宁省兴城市人,硕士,主要从事腹泻病防治与研究工作;
    通信作者: 阚飙, kanbiao@icdc.cn
  • 基金项目: 国家科技重大专项(No.2008ZX10004-002)

摘要: 在引起伤寒、副伤寒的病原中,甲型副伤寒沙门菌所占的比例逐渐升高。我国每年均有不同程度的地方性甲型副伤寒流行及暴发,东南亚地区甲型副伤寒流行情况日趋严重。由于现在还没有针对甲型副伤寒沙门菌的疫苗,所以具有免疫原性蛋白的筛选及减毒全菌疫苗、亚单位疫苗的研制逐渐成为热点。随着甲型副伤寒沙门菌ATCC9150全基因组序列的公布,利用基因组和蛋白质组学的方法为研究甲型副伤寒沙门菌的疫苗靶标提供了良好的基础。本文综述了疫苗靶标蛋白的识别方法以及可能用于鉴别诊断或者疫苗有效靶标的潜在甲型副伤寒沙门菌抗原蛋白。

English


    1. [1] Crump JA, Luby SP, Mintz ED.The global burden of typhoid fever
      [J]. Bull World Health Organ,2004,82(5):346-353.

      [2] Ochiai RL, Wang X, von Seidlein L, et al, Salmonella paratyphi A rates, Asia
      [J]. Emerg Infect Dis,2005,11(11):1764-1766.

      [3] Yang HH,Gong J,Zhang J, et al. An outbreak of Salmonella paratyphi A in a boarding school:a community-acquired enteric fever and carriage investigation
      [J]. Epidemiol Infect,2010,138(12):1765-1774.

      [4] Woods CW, Murdoch DR, Zimmerman MD, et al. Emergence of Salmonella enterica serotype paratyphi A as a major cause of enteric fever in Kathmandu, Nepal
      [J].Trans R Soc Trop Med Hyg,2006,100(11):1063-1067.

      [5] Connor BA, Schwartz E. Typhoid and paratyphoid fever in travelers
      [J]. Lancet Infect Dis,2005,5(10):623-628.

      [6] Meltzer E, Sadik C, Schwartz E. Enteric fever in Israeli travelers:a nationwide study
      [J]. J Travel Med,2005,12(5):275-281.

      [7] Verma S, Thakur S, Kanga A, et al. Emerging Salmonella paratyphi A enteric fever and changing trends in antimicrobial resistance pattern of Salmonella in Shimla
      [J]. Indian J Med Microbiol,2010,28(1):51-53.

      [8] Molloy A, Nair S, Cooke FJ,et al. First report of Salmonella enterica serotype paratyphi a azithromycin resistance leading to treatment failure.2010
      [J]. J Clin Microbiol,2010, 48(12):4655-4657.

      [9] Guzman CA, Borsutzky S, Griot-Wenk M, et al. Vaccines against typhoid fever
      [J]. Vaccine,2006,24(18):3804-3811.

      [10] Levine MM, Ferreccio C, Black RE, et al.Ty21a live oral typhoid vaccine and prevention of paratyphoid fever caused by Salmonella enterica serovar paratyphi B
      [J]. Clin Infect Dis,2007,45 Suppl 1:S24-28.

      [11] Roland KL, Tinge SA, Kochi SK, et al. Reactogenicity and immunogenicity of live attenuated Salmonella enterica serovar paratyphi A enteric fever vaccine candidates
      [J].Vaccine,2010, 28(21):3679-3687.

      [12] Konadu EY, Lin FY, Hó VA, et al. Phase 1 and phase 2 studies of Salmonella enterica serovar paratyphi A O-specific polysaccharide-tetanus toxoid conjugates in adults, teenagers, and 2-to 4-year-old children in Vietnam
      [J].Infect Immun,2000,68(3):1529-1534.

      [13] McClelland M, Sanderson KE, Clifton SW, et al. Comparison of genome degradation in Paratyphi A and Typhi, human-restricted serovars of Salmonella enterica that cause typhoid
      [J]. Nat Genet,2004,36(12):1268-1274.

      [14] Etz H, Minh DB, Henics T, et al. Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus
      [J].Proc Natl Acad Sci USA,2002,99(10):6573-6578.

      [15] Li B, Jiang L, Song Q, et al. Protein microarray for profiling antibody responses to Yersinia pestis live vaccine
      [J].Infect Immun,2005,73(6):3734-3739.

      [16] Kudva IT, Krastins B, Sheng H, et al. Proteomics-based expression library screening (PELS):a novel method for rapidly defining microbial immunoproteomes
      [J].Mol Cell Proteomics,2006,5(8):1514-1519.

      [17] Charles RC, Sheikh A, Krastins B, et al. Characterization of anti-Salmonella enterica serotype Typhi antibody responses in bacteremic Bangladeshi patients by an immunoaffinity proteomics-based technology
      [J].Clin Vaccine Immunol,2010,17(8):1188-1195.

      [18] Pizza M, Scarlato V, Masignani V, et al. Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing
      [J].Science,2000,287(5459):1816-1820.

      [19] Wizemann TM, Heinrichs JH, Adamou JE, et al. Use of a whole genome approach to identify vaccine molecules affording protection against Streptococcus pneumoniae infection
      [J].Infect Immun,2001,69(3):1593-1598.

      [20] Wang B. Immunoproteomics of outer membrane proteins of Salmonella serotype paratyphi A CMCC_50973 . Beijing:Academy of Military Medical Science,2006.(in Chinese) 王斌. 甲型副伤寒沙门氏菌CMCC_50973外膜蛋白的免疫蛋白质组学研究 . 北京:军事医学科学院,2006.

      [21] Wang HW,Zhao P,Zhang M, et al.Cloning and functional analysis of promoter pagC from attenuated Salmonella typhimurium
      [J].Acta Microbiologica Sinica,2003,43(3):308-314.(in Chinese) 王宏卫,赵平,张珉,等.鼠伤寒沙门菌pagC启动子的克隆与活性分析.微生物学报,2003,43(3):308-314.

      [22] Liu CM. Expression and immunoprotection of outer membrane proteins of Salmonella paratyphi A . Jiangxi:Nanchang University,2007. 刘春梅, 甲型副伤寒沙门氏菌外膜蛋白的表达及其免疫保护性研究 . 江西:南昌大学,2007.

      [23] Libby SJ, Goebel W, Ludwig A, et al. A cytolysin encoded by Salmonella is required for survival within macrophages
      [J]. Proc Natl Acad Sci USA,1994,91(2):489-493.

      [24] Rhein C von, Hunfeld KP, Ludwig A. Serologic evidence for effective production of cytolysin A in Salmonella enterica serovars typhi and paratyphi A during human infection
      [J]. Infect Immun,2006,74(11):6505-6508.

      [25] Ruan P, Xia XP, Sun D, et al. Recombinant SpaO and H1a as immunogens for protection of mice from lethal infection with Salmonella paratyphi A:implications for rational design of typhoid fever vaccines
      [J].Vaccine,2008,26(51):6639-6644.

      [26] Korbsrisate S, Sarasombath S, Praaporn N, et al. Detection of IgM antibody against region IV flagellin of Salmonella paratyphi A
      [J].Southeast Asian J Trop Med Public Health,1998, 29(4):864-871.

      [27] Harris JB, Baresch-Bernal A, Rollins SM, et al. Identification of in vivo-induced bacterial protein antigens during human infection with Salmonella enterica serovar Typhi
      [J]. Infect Immun,2006,74(9):5161-5168.


    1. [1]

      申小娜海荣俞东征 . 鼠疫菌基因组学研究进展. 疾病监测, 2009, 24(6): 440-445. DOI: 10.3784/j.issn.1003-9961.2009.06.019

    2. [2]

      俞东征 . 鼠疫与假结核耶尔森菌基因组比较分析. 疾病监测, 2011, 26(10): 760-765.

    3. [3]

      李马超刘海灿赵秀琴万康林 . 卡介苗中PE和PPE家族蛋白B细胞抗原表位多态性研究. 疾病监测, 2016, 31(4): 282-287. DOI: 10.3784/j.issn.1003-9961.2016.04.006

    4. [4]

      田克诚阚飙吕太富游旅张玉琼 . 贵州省2000年甲型副伤寒沙门菌分离株16SrRNA基因型分析. 疾病监测, 2001, 16(12): 445-448. DOI: 10.3784/j.issn.1003-9961.2001.12.445

    5. [5]

      王鸣柳闫梅英阚飙樊粉霞 . 基于单一基因的反转录-环介导等温核酸扩增技术快速检测甲型副伤寒沙门菌. 疾病监测, 2014, 29(9): 752-757. DOI: 10.3784/j.issn.1003-9961.2014.09.021

    6. [6]

      徐帅侯雪新孙丽娜张景山吉兴照唐璐韦超李和桥王雪冰李振军 . 鼻疽诺卡菌菌体蛋白的免疫蛋白质组学研究. 疾病监测, 2018, 33(4): 294-299. DOI: 10.3784/j.issn.1003-9961.2018.04.009

    7. [7]

      杨晋川夏杨刘金芳许静静郭惠童晶周艳秋 . 一起甲型副伤寒沙门菌污染水源所致腹泻暴发疫情的病原学调查. 疾病监测, 2006, 21(9): 463-467. DOI: 10.3784/j.issn.1003-9961.2006.9.463

    8. [8]

      李爱华叶长芸 . 单核细胞增生性李斯特菌致病相关机制的研究进展. 疾病监测, 2011, 26(11): 914-919. DOI: 10.3784/j.issn.1003-9961.2011.11.023

    9. [9]

      王蓓崔玉明赵季文徐萃瑜张莹 . 生殖支原体抗原快速检测方法的初步建立与应用. 疾病监测, 1999, 14(12): 445-449. DOI: 10.3784/j.issn.1003-9961.1999.12.445

    10. [10]

      姚颖波曾丽萍王树坤阚飙刁保卫崔志刚吴强杨汝松刘红雁 . 云南省玉溪市甲型副伤寒沙门菌PFGE数据库的建立及其应用. 疾病监测, 2009, 24(10): 770-773. DOI: 10.3784/j.issn.1003-9961.2009.10.015

    11. [11]

      李杰肖燕樊粉霞阚飙闫梅英 . 利用双重TaqMan荧光定量聚合酶链反应技术鉴别诊断粪便标本中的伤寒和甲型副伤寒沙门菌. 疾病监测, 2014, 29(4): 310-315. DOI: 10.3784/j.issn.1003-9961.2014.04.015

    12. [12]

      姚颖波左顺武曾丽萍刘红雁孔丽霞杨汝松王树坤 . 云南省玉溪市甲型副伤寒沙门菌对抗生素的敏感性分析. 疾病监测, 2013, 28(2): 140-143. DOI: 10.3784/j.issn.1003-9961.2013.2.016

    13. [13]

      朱水荣褚佩英张政杨仕贵 . 脉冲场凝胶电泳与质粒图谱分析应用于浙江省甲副伤寒菌株的分子流行病学调查. 疾病监测, 2005, 20(9): 463-467. DOI: 10.3784/j.issn.1003-9961.2005.9.463

    14. [14]

      顾超慧崔步云关平原 . 蛋白质组学在布鲁氏菌病研究中的应用. 疾病监测, 2009, 24(5): 373-378. DOI: 10.3784/j.issn.1003-9961.2009.05.020

    15. [15]

      王建平杜鹏程白雪梅郑翰 . 猪链球菌wzy基因与血清型别的相关性分析. 疾病监测, 2018, 33(12): 990-994. DOI: 10.3784/j.issn.1003-9961.2018.12.006

    16. [16]

      李兴隆郭慧芬牛玉梅关彩萍 . 2007年山西省太原市健康人群乙型病毒性肝炎免疫水平监测分析. 疾病监测, 2010, 25(11): 891-893.

    17. [17]

      谢芳 (综述)海荣 (审校)俞东征 (审校) . 鼠疫耶尔森菌基因组重排研究进展. 疾病监测, 2010, 25(5): 390-393. DOI: 10.3784/j.issn.1003-9961.2010.05.016

    18. [18]

      张恩民王琪申小娜陈晨俞东征海荣 . 鼠疫菌基因组"默认编码序列" 数据库的建立与初步研究. 疾病监测, 2010, 25(6): 436-439. DOI: 10.3784/j.issn.1003-9961.2010.06.005

    19. [19]

      罗雪莲王海印叶长芸 . 单增李斯特菌EGD-e基因组的密码子使用分析. 疾病监测, 2011, 26(4): 290-294.

    20. [20]

      宋韩李振军吉兴照孙丽娜徐帅韩李超郑宁伟楼永良 . 鼻疽诺卡菌Nfa34810蛋白的抗原表位筛选与鉴定. 疾病监测, 2020, 35(6): 540-546. DOI: 10.3784/j.issn.1003-9961.2020.06.018

  • 加载中
计量
  • PDF下载量:  664
  • 文章访问数:  898
  • HTML全文浏览量:  4
  • 引证文献数: 0
文章相关
  • 通信作者:  阚飙, kanbiao@icdc.cn
  • 收稿日期:  2011-05-23
  • 刊出日期:  2011-07-25
通信作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

/

返回文章

在线交流