一例狂犬病病例的实验室诊断及其启示

李幸乐 黄学勇 孙建伟 吕新军

李幸乐, 黄学勇, 孙建伟, 吕新军. 一例狂犬病病例的实验室诊断及其启示[J]. 疾病监测, 2014, 29(3): 186-190. doi: 10.3784/j.issn.1003-9961.2014.03.006
引用本文: 李幸乐, 黄学勇, 孙建伟, 吕新军. 一例狂犬病病例的实验室诊断及其启示[J]. 疾病监测, 2014, 29(3): 186-190. doi: 10.3784/j.issn.1003-9961.2014.03.006
LI Xing-le, HUANG Xue-yong, SUN Jian-wei, LYU Xin-jun. Laboratory diagnosis of a human rabies case and its significance[J]. Disease Surveillance, 2014, 29(3): 186-190. doi: 10.3784/j.issn.1003-9961.2014.03.006
Citation: LI Xing-le, HUANG Xue-yong, SUN Jian-wei, LYU Xin-jun. Laboratory diagnosis of a human rabies case and its significance[J]. Disease Surveillance, 2014, 29(3): 186-190. doi: 10.3784/j.issn.1003-9961.2014.03.006

一例狂犬病病例的实验室诊断及其启示

doi: 10.3784/j.issn.1003-9961.2014.03.006
基金项目: 

农业公益性行业科研专项经费项目-狂犬病监测及疫情报告系统研究(No.201103032)

Laboratory diagnosis of a human rabies case and its significance

Funds: 

This study was supported by Grants from the Scientific Research Project in Agriculture and Public Welfare: Research on Rabies Surveillance and Reporting System (No.201103032)

  • 摘要: 目的 通过1例人狂犬病病例的实验室确诊探讨人狂犬病病例实验室诊断的可行性及其对狂犬病防控的意义。方法 对存活疑似人狂犬病病例的唾液、脑脊液、血清标本采用反转录-聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)、快速荧光灶抑制试验(rapid fluorescent focus inhibition test,RFFIT)进行实验室确诊;通过RFFIT对密切接触个例血清抗狂犬病病毒中和抗体进行检测。结果 病例唾液标本用RT-PCR方法检测获得狂犬病病毒核蛋白、RNA聚合酶基因预期扩增片段,序列测定和分析进一步证实为狂犬病病毒。病例血清标本经RFFIT检测抗狂犬病病毒中和抗体阳性。1名病例密切接触者按照狂犬病暴露进行暴露后预防处置,血清抗狂犬病病毒中和抗体检测为阳性。结论 1例疑似人狂犬病病例经实验室诊断确诊为狂犬病病例,实验室检测结果对指导个案处置有现实意义。
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出版历程
  • 收稿日期:  2013-12-31
  • 刊出日期:  2014-03-20

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