一例狂犬病病例的实验室诊断及其启示

李幸乐; 黄学勇; 孙建伟; 吕新军

doi:10.3784/j.issn.1003-9961.2014.03.006

一例狂犬病病例的实验室诊断及其启示

doi: 10.3784/j.issn.1003-9961.2014.03.006

基金项目:

农业公益性行业科研专项经费项目-狂犬病监测及疫情报告系统研究（No.201103032）

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出版历程
- 收稿日期: 2013-12-31
- 刊出日期: 2014-03-20

Laboratory diagnosis of a human rabies case and its significance

Funds:

This study was supported by Grants from the Scientific Research Project in Agriculture and Public Welfare: Research on Rabies Surveillance and Reporting System (No.201103032)

摘要

摘要: 目的通过1例人狂犬病病例的实验室确诊探讨人狂犬病病例实验室诊断的可行性及其对狂犬病防控的意义。方法对存活疑似人狂犬病病例的唾液、脑脊液、血清标本采用反转录－聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）、快速荧光灶抑制试验（rapid fluorescent focus inhibition test，RFFIT）进行实验室确诊；通过RFFIT对密切接触个例血清抗狂犬病病毒中和抗体进行检测。结果病例唾液标本用RT-PCR方法检测获得狂犬病病毒核蛋白、RNA聚合酶基因预期扩增片段，序列测定和分析进一步证实为狂犬病病毒。病例血清标本经RFFIT检测抗狂犬病病毒中和抗体阳性。1名病例密切接触者按照狂犬病暴露进行暴露后预防处置，血清抗狂犬病病毒中和抗体检测为阳性。结论 1例疑似人狂犬病病例经实验室诊断确诊为狂犬病病例，实验室检测结果对指导个案处置有现实意义。
- 狂犬病 /
- 反转录－聚合酶链反应 /
- 快速荧光灶抑制试验 /
- 实验室诊断
Abstract: Objective To evaluate the feasibility of laboratory diagnosis of human rabies and its significance for prevention and control of rabies. Methods Laboratory diagnosis of a human rabies cases was conducted by using reverse transcription polymerase chain reaction (RT-PCR) and rapid fluorescent focus inhibition test (RFFIT) to detect the saliva, cerebrospinal fluid (CSF), serum samples collected from the patients. Results The predicted segments of the nucleoprotein (N), RNA-dependent RNA polymerase (RdRp)/Large protein (L) genes of rabies virus were amplified from the saliva samples with RT-PCR. Sequencing and phylogenetic analysis confirmed the rabies virus infection. Rabies virus neutralizing antibody (RVNP) was detected with RFFIT in the serum sample. One close contact received rabies postexposure prophylaxis (PEP). The RVNP of the contact was positive. Conclusion A suspected human rabies case was laboratory confirmed. Laboratory test results has important significance for the treatment of rabies cases.
- rabies /
- reverse transcription polymerase chain reaction /
- rapid fluorescent focus inhibition test /
- laboratory diagnosis

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参考文献(21)

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文章访问数: 1743
HTML全文浏览量: 65
PDF下载量: 240
被引次数: 0

一例狂犬病病例的实验室诊断及其启示

doi: 10.3784/j.issn.1003-9961.2014.03.006

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出版历程

Laboratory diagnosis of a human rabies case and its significance

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出版历程

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