巴尔通体体外药敏试验方法研究

宋秀平 刘起勇 栗冬梅 孙继民 吴凡 黄儒婷

宋秀平, 刘起勇, 栗冬梅, 孙继民, 吴凡, 黄儒婷. 巴尔通体体外药敏试验方法研究[J]. 疾病监测, 2014, 29(11): 905-910. doi: 10.3784/j.issn.1003-9961.2014.11.016
引用本文: 宋秀平, 刘起勇, 栗冬梅, 孙继民, 吴凡, 黄儒婷. 巴尔通体体外药敏试验方法研究[J]. 疾病监测, 2014, 29(11): 905-910. doi: 10.3784/j.issn.1003-9961.2014.11.016
SONG Xiu-ping, LIU Qi-yong, LI Dong-mei, SUN Ji-min, WU Fan, HUANG Ru-ting. Comparison of two methods in vitro test of drug susceptibility of Bartonella[J]. Disease Surveillance, 2014, 29(11): 905-910. doi: 10.3784/j.issn.1003-9961.2014.11.016
Citation: SONG Xiu-ping, LIU Qi-yong, LI Dong-mei, SUN Ji-min, WU Fan, HUANG Ru-ting. Comparison of two methods in vitro test of drug susceptibility of Bartonella[J]. Disease Surveillance, 2014, 29(11): 905-910. doi: 10.3784/j.issn.1003-9961.2014.11.016

巴尔通体体外药敏试验方法研究

doi: 10.3784/j.issn.1003-9961.2014.11.016
基金项目: 

重大传染病防治基金资助课题(No. 2008ZX10004-010)

国家自然科学基金(No.81101286)

Comparison of two methods in vitro test of drug susceptibility of Bartonella

Funds: 

This study was supported by the the National Science and Technology Key Project for priority communicable disease prevention and control project (No.2008ZX10004-010) and the National Natural Science Foundation of China (No. 81101286)

  • 摘要: 目的 优化巴尔通体体外药敏试验方法,检测多种抗生素对巴尔通体的最低抑菌浓度(MICs),并分析耐药性.方法 优化Etest法试验条件;将该方法与琼脂稀释法进行比较,评价该方法的可靠性.采用Etest法,检测了7株ATCC参考株对22种抗生素的MICs,数据分析使用SPSS 13.0软件.结果 确定含5%羊血的胰酶大豆琼脂培养基为最佳培养基、2.0 MCF(麦氏浊度)度为最佳细菌接种浓度,7天为最佳孵育时间.测试的7株巴尔通体在体外对强力霉素等13种抗生素敏感(MIC结论 Etest法简便易行、定值准确、重复性好、结果可靠,可以用于巴尔通体体外药敏试验,并获得了巴尔通体的一些体外药敏数据,为将来制订巴尔通体药敏标准提供一定的依据.
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出版历程
  • 收稿日期:  2014-06-20
  • 刊出日期:  2014-11-20

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