肠道病毒71型湖州分离株的遗传进化和重组分析

吴晓芳 纪蕾 徐德顺 陈莉萍 沈月华 朱晓娟 查赟峰

吴晓芳, 纪蕾, 徐德顺, 陈莉萍, 沈月华, 朱晓娟, 查赟峰. 肠道病毒71型湖州分离株的遗传进化和重组分析[J]. 疾病监测, 2016, 31(7): 566-570. doi: 10.3784/j.issn.1003-9961.2016.07.008
引用本文: 吴晓芳, 纪蕾, 徐德顺, 陈莉萍, 沈月华, 朱晓娟, 查赟峰. 肠道病毒71型湖州分离株的遗传进化和重组分析[J]. 疾病监测, 2016, 31(7): 566-570. doi: 10.3784/j.issn.1003-9961.2016.07.008
WU Xiao-fang, JI Lei, XU De-shun, CHEN Li-ping, SHEN Yue-hua, ZHU Xiao-juan, ZHA Yun-feng. Genetic evolution and recombination of enterovirus 71 isolated in Huzhou[J]. Disease Surveillance, 2016, 31(7): 566-570. doi: 10.3784/j.issn.1003-9961.2016.07.008
Citation: WU Xiao-fang, JI Lei, XU De-shun, CHEN Li-ping, SHEN Yue-hua, ZHU Xiao-juan, ZHA Yun-feng. Genetic evolution and recombination of enterovirus 71 isolated in Huzhou[J]. Disease Surveillance, 2016, 31(7): 566-570. doi: 10.3784/j.issn.1003-9961.2016.07.008

肠道病毒71型湖州分离株的遗传进化和重组分析

doi: 10.3784/j.issn.1003-9961.2016.07.008

Genetic evolution and recombination of enterovirus 71 isolated in Huzhou

  • 摘要: 目的 对湖州市肠道病毒71型(EV71)进行基因特征及重组特点分析。方法 对2011-2012年湖州市EV71分离株进行全基因组测序,并与其基因型及A组肠道病毒其他血清型的原型株进行全序列比对,使用Mega 4.0软件绘制系统进化树。然后利用Simplot 3.5.1软件进行相似性分析,对基因重组加以验证。结果 P1区系统进化分析显示,2011-2012年湖州市EV71株属于C4基因型的C4a亚型。P1、P2、P3区的系统进化和相似性分析证实湖州市分离株在非结构蛋白2B编码区存在EV71 C基因型和Cox A16、14、4型间重组。结论 2011-2012年湖州市EV71分离株为C4a亚型,与2004年以来中国大陆优势株流行趋势一致。EV71 C4基因型湖州市株存在型间重组现象。
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出版历程
  • 收稿日期:  2015-11-06
  • 刊出日期:  2016-07-20

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