羊瘙痒因子139A感染引起小鼠脑组织中的1-ACT表达增加

徐小峰 张仁庆 马月 吕燕 黎建乐 石强 肖康 孙静 杨晓东 董小平 陈操

徐小峰, 张仁庆, 马月, 吕燕, 黎建乐, 石强, 肖康, 孙静, 杨晓东, 董小平, 陈操. 羊瘙痒因子139A感染引起小鼠脑组织中的1-ACT表达增加[J]. 疾病监测, 2017, 32(4): 282-286. doi: 10.3784/j.issn.1003-9961.2017.04.007
引用本文: 徐小峰, 张仁庆, 马月, 吕燕, 黎建乐, 石强, 肖康, 孙静, 杨晓东, 董小平, 陈操. 羊瘙痒因子139A感染引起小鼠脑组织中的1-ACT表达增加[J]. 疾病监测, 2017, 32(4): 282-286. doi: 10.3784/j.issn.1003-9961.2017.04.007
XU Xiao-feng, ZHANG Ren-qing, MA Yue, LYU Yan, LI Jian-le, SHI Qiang, XIAO Kang, SUN Jing, YANG Xiao-dong, Dong Xiao-ping, CHEN Cao. Remarkable up-regulation of 1-antichymotrypsin in brains of scrapie agent 139A infected mice[J]. Disease Surveillance, 2017, 32(4): 282-286. doi: 10.3784/j.issn.1003-9961.2017.04.007
Citation: XU Xiao-feng, ZHANG Ren-qing, MA Yue, LYU Yan, LI Jian-le, SHI Qiang, XIAO Kang, SUN Jing, YANG Xiao-dong, Dong Xiao-ping, CHEN Cao. Remarkable up-regulation of 1-antichymotrypsin in brains of scrapie agent 139A infected mice[J]. Disease Surveillance, 2017, 32(4): 282-286. doi: 10.3784/j.issn.1003-9961.2017.04.007

羊瘙痒因子139A感染引起小鼠脑组织中的1-ACT表达增加

doi: 10.3784/j.issn.1003-9961.2017.04.007
基金项目: 

国家自然科学基金(No.81401670,81630062);中国疾病预防控制中心青年科研基金(No.2016A101);传染病预防控制国家重点实验室(No.2012SKLID102,2016SKLID603)

Remarkable up-regulation of 1-antichymotrypsin in brains of scrapie agent 139A infected mice

  • 摘要: 目的 探究1-ACT在羊瘙痒因子139A感染小鼠脑组织中的变化情况。方法 利用蛋白免疫印迹、免疫组织化学、间接免疫荧光及荧光共聚焦方法分析羊瘙痒因子139A感染小鼠脑组织中1-ACT表达的变化和分布特点。结果 蛋白免疫印迹方法显示在羊瘙痒因子139A感染小鼠终末期脑组织中1-ACT的含量较正常对照小鼠明显上调且随着潜伏期的延长而逐渐增加;免疫组织化学方法发现1-ACT主要分布于羊瘙痒因子139A感染小鼠的皮层、丘脑和小脑区域;间接免疫荧光实验显示羊瘙痒因子139A感染终末期小鼠脑组织中补体成分C3含量明显增加,同时荧光共聚焦实验表明1-ACT与补体成分C3存在明显的共定位现象。结论 羊瘙痒因子139A感染终末期小鼠脑组织中1-ACT含量明显增加。
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出版历程
  • 收稿日期:  2017-01-20
  • 刊出日期:  2017-04-20

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