江西省赣州市两株冠状病毒的全基因组遗传特征分析

管晓庆 廖勇 李建华 王文 陈志海

管晓庆, 廖勇, 李建华, 王文, 陈志海. 江西省赣州市两株冠状病毒的全基因组遗传特征分析[J]. 疾病监测, 2017, 32(4): 323-327. doi: 10.3784/j.issn.1003-9961.2017.04.016
引用本文: 管晓庆, 廖勇, 李建华, 王文, 陈志海. 江西省赣州市两株冠状病毒的全基因组遗传特征分析[J]. 疾病监测, 2017, 32(4): 323-327. doi: 10.3784/j.issn.1003-9961.2017.04.016
GUAN Xiao-qing, LIAO Yong, LI Jian-hua, WANG Wen, CHEN Zhi-hai. Genetic analysis of complete genome of two coronaviruses strains isolated in Ganzhou, Jiangxi[J]. Disease Surveillance, 2017, 32(4): 323-327. doi: 10.3784/j.issn.1003-9961.2017.04.016
Citation: GUAN Xiao-qing, LIAO Yong, LI Jian-hua, WANG Wen, CHEN Zhi-hai. Genetic analysis of complete genome of two coronaviruses strains isolated in Ganzhou, Jiangxi[J]. Disease Surveillance, 2017, 32(4): 323-327. doi: 10.3784/j.issn.1003-9961.2017.04.016

江西省赣州市两株冠状病毒的全基因组遗传特征分析

doi: 10.3784/j.issn.1003-9961.2017.04.016
基金项目: 

生物安全关键技术研发(No.2016YFC1201901)

Genetic analysis of complete genome of two coronaviruses strains isolated in Ganzhou, Jiangxi

Funds: 

This study was supported by the National Special Project on Research and Development of Key Biosafety Technologies (No.2016YFC1201901)

  • 摘要: 目的 分析江西省赣州市2株亚洲鼩鼱携带冠状病毒的全基因组遗传特征。方法 用反转录-聚合酶链式反应及RACE方法从亚洲鼩鼱粪便中扩增其全基因组;利用MegAlign软件进行序列同源性分析;利用phyML软件构建系统发育树。结果 全基因组的同源性分析表明毒株GZ01、GZ02与已知的亚洲鼩鼱携带冠状病毒的序列具有很高的同源性(88.6%~92.1%)。系统发育分析表明毒株GZ01、GZ02与上述序列有最近的亲缘关系。结论 赣州市亚洲鼩鼱携带属冠状病毒,与已知的毒株为同一种冠状病毒,系统发育分析发现亚洲鼩鼱在冠状病毒的起源与进化过程中起着重要作用。
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出版历程
  • 收稿日期:  2017-02-27
  • 刊出日期:  2017-04-20

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