詹亚惠, 栾琳, 张梦寒. 2011-2016年江苏省苏州市脑膜炎奈瑟菌分子流行特征分析[J]. 疾病监测, 2018, 33(8): 640-643. DOI: 10.3784/j.issn.1003-9961.2018.08.007
引用本文: 詹亚惠, 栾琳, 张梦寒. 2011-2016年江苏省苏州市脑膜炎奈瑟菌分子流行特征分析[J]. 疾病监测, 2018, 33(8): 640-643. DOI: 10.3784/j.issn.1003-9961.2018.08.007
Yahui Zhan, Lin Luan, Menghan Zhang. Analysis on molecular epidemiological characteristics of Neisseria meningitidis in Suzhou, Jiangsu, 2011–2016[J]. Disease Surveillance, 2018, 33(8): 640-643. DOI: 10.3784/j.issn.1003-9961.2018.08.007
Citation: Yahui Zhan, Lin Luan, Menghan Zhang. Analysis on molecular epidemiological characteristics of Neisseria meningitidis in Suzhou, Jiangsu, 2011–2016[J]. Disease Surveillance, 2018, 33(8): 640-643. DOI: 10.3784/j.issn.1003-9961.2018.08.007

2011-2016年江苏省苏州市脑膜炎奈瑟菌分子流行特征分析

Analysis on molecular epidemiological characteristics of Neisseria meningitidis in Suzhou, Jiangsu, 2011–2016

  • 摘要:
    目的 对2011 – 2016年江苏省苏州市分离于患者、健康人群和密切接触者的脑膜炎奈瑟菌(Nm)进行血清群鉴定,了解苏州市Nm的血清群及遗传进化关系。
    方法 采用乳胶凝集和多位点序列分型方法,对分离到的33株Nm的7个管家基因序列进行测定,并与PubMLST数据库中序列进行比对,确定等位基因谱型及序列型,采用Cluster 3.0分析菌株间的进化关系。
    结果 33株Nm中B群占66.67%(22/33),W135群占24.24%(8/33),C群、Y群和未分群各占3.03%(1/33);2011年分离的3株W135群属于高致病性克隆系ST-11 complex/ET-37 complex,2012 – 2013年分离的B群5株属于高致病性克隆系ST-4821 complex。
    结论 2011 – 2016年苏州市Nm主要以B群为主,W135群次之,基因型以多克隆系并存,出现高致病性克隆系ST-11 complex/ET-37 complex W135群和ST-4821 complex B群,提示应密切监测菌株型别,预防流行性脑脊髓膜炎的暴发流行。

     

    Abstract:
    Objective To understand the serogroup and molecular epidemiological characteristics of Neisseria meningitidis isolated from patients, close contacts and healthy carriers in Suzhou during 2011–2013.
    Methods Latex agglutination test and multi locus sequence typing (MLST) were conducted to identify the serogroups and sequences of 7 housekeeping genes of 33 strains of N. meningitidis isolated in Suzhou during 2011–2013. The sequence alignment was conducted by using PubMLST database and the allelic profile and sequence types were determined. Software Cluster 3.0 was used in analyzing genetic evolution of the strains.
    Results Among the N. meningitidis strains isolated, 22 were serogroup B strains (66.67%), 8 were serogroup W135 strains (24.24%), 1 was serogroup C strain (3.03%) 1 was serogroup Y strains (3.03%) and 1 was unspecified (3.03%). Three serogroup W135 strains isolated in 2011 belonged to highly pathogenic ST-11 complex/ET-37 complex and 5 serogroup B strains isolated during 2012–2013 belonged to highly pathogenic ST-4821complex.
    Conclusion N. meningitidis B was predominant, followed by N. meningitidis W135, in Suzhou during 2011–2016. Multiple clonal complexes existed. Highly pathogenic serogroup W135 strains belonging to ST-11 complex/ET-37 complex and serogroup B strains belonging to ST-4821 complex have appeared in Suzhou, suggesting the importance of strengthening the surveillance to prevent and control the outbreak of meningococcal meningitis.

     

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