Abstract: A total of 12 307 strains of pathogenic bacteria firstly isolated form the patients in intensive care unit (ICU) and general wards in a hospital in 2016 were collected. The distribution of bacteria and their drug resistance were analyzed. Acinetobacter baumannii (25.50%) was the isolated pathogen ranking first in ICU, while Pseudomonas aeruginosa (16.68%) was the isolated pathogen ranking first in general wards. The antibiotic resistant rate of the pathogens from ICU was higher than that of the pathogens from general wards, and severe multi drug resistance was detected in the pathogen strains from ICU. It is necessary to standardize the antibiotic use strengthen the prevention of nosocomial infection to control the prevalence of drug resistance in ICU.