2012-2014年乌鲁木齐市5岁以下急性腹泻儿童患者诺如病毒感染及基因型研究

沙比热木·托合塔木 肖克来提·努尔 周海健 靳淼 孔翔羽 周永康 李慧莹 段招军 马合木提

沙比热木·托合塔木, 肖克来提·努尔, 周海健, 靳淼, 孔翔羽, 周永康, 李慧莹, 段招军, 马合木提. 2012-2014年乌鲁木齐市5岁以下急性腹泻儿童患者诺如病毒感染及基因型研究[J]. 疾病监测, 2018, 33(10): 814-818. doi: 10.3784/j.issn.1003-9961.2018.10.006
引用本文: 沙比热木·托合塔木, 肖克来提·努尔, 周海健, 靳淼, 孔翔羽, 周永康, 李慧莹, 段招军, 马合木提. 2012-2014年乌鲁木齐市5岁以下急性腹泻儿童患者诺如病毒感染及基因型研究[J]. 疾病监测, 2018, 33(10): 814-818. doi: 10.3784/j.issn.1003-9961.2018.10.006
Shabiremu·Tuohetamu, Xiaokelaiti·Nuer, Haijian Zhou, Miao Jin, Xiangyu Kong, Yongkang Zhou, Huiying Li, Zhaojun Duan, Mahemuti. Molecular epidemiology of norovirus infection in hospitalized children with acute gastroenteritis in Urumqi, Xinjiang, 2012−2014[J]. Disease Surveillance, 2018, 33(10): 814-818. doi: 10.3784/j.issn.1003-9961.2018.10.006
Citation: Shabiremu·Tuohetamu, Xiaokelaiti·Nuer, Haijian Zhou, Miao Jin, Xiangyu Kong, Yongkang Zhou, Huiying Li, Zhaojun Duan, Mahemuti. Molecular epidemiology of norovirus infection in hospitalized children with acute gastroenteritis in Urumqi, Xinjiang, 2012−2014[J]. Disease Surveillance, 2018, 33(10): 814-818. doi: 10.3784/j.issn.1003-9961.2018.10.006

2012-2014年乌鲁木齐市5岁以下急性腹泻儿童患者诺如病毒感染及基因型研究

doi: 10.3784/j.issn.1003-9961.2018.10.006
基金项目: 新疆少数民族科技人才特殊培养计划(No. 2017D03008)
详细信息
    作者简介:

    沙比热木·托合塔木,女,新疆维吾尔自治区伊宁县人,副主任医师,主要从事传染病预防、控制与检测

    通讯作者:

    马合木提,Tel:0991–2621105,Email:mahefyk@163.com

  • 中图分类号: R373.2

Molecular epidemiology of norovirus infection in hospitalized children with acute gastroenteritis in Urumqi, Xinjiang, 2012−2014

Funds: This study was supported by the fund for the Special Project of Scientific Research Talent Training in Minorities (No. 2017D03008)
More Information
  • 摘要: 目的了解2012 — 2014年新疆维吾尔自治区(新疆)乌鲁木齐市<5岁住院儿童患者诺如病毒的分子流行病学特征。方法收集2012 — 2014年乌鲁木齐市895例<5岁急性腹泻儿童患者粪便标本和流行病学资料,应用实时荧光反转录聚合酶链式反应(RT-PCR)进行诺如病毒检测,阳性样本通过RT-PCR扩增、测序和序列分析。结果诺如病毒的检出率为16.8%(150/895),不同年份阳性率差异有统计学意义(χ2=21.080,P<0.05);不同年龄组阳性率差异有统计学意义(χ2=13.367,P=0.020)。 137份样本获得聚合酶区序列,其中89份获得衣壳蛋白区序列。聚合酶区和衣壳区分别包括11和9个基因型。根据89株双分型结果,GⅡ.4 Den Haag 2006b和GⅡ.Pe/GⅡ.4 Sydney 2012分别占21.3%和32.6%,GⅡ.P12/GⅡ.3占29.2%。 根据衣壳区分型结果,在GⅡ.4基因型中,2012 — 2014年GⅡ.4 DenHaag 2006b所占比例分别为90.0%、0%和0%;GⅡ.Pe/GⅡ.4 Sydney 2012所占比例分别为10.0%、100%和100%。结论新疆乌鲁木齐市急性腹泻儿童诺如病毒具有遗传多样性;GⅡ.Pe/GⅡ.4 Sydney 2012在新疆地区出现,逐渐替代GⅡ.4 DenHaag 2006b成为新的流行优势株。
  • 图  1  2012-2014年乌鲁木齐市<5岁腹泻儿童诺如病毒季节分布

    Figure  1.  Seasonal distribution of norovirus detection in diarrheal children aged <5 years in Urumqi, 2012−2014

    图  2  2012-2014年乌鲁木齐市<5岁腹泻儿童诺如病毒聚合酶基因型分布

    Figure  2.  Annual genotype distribution in polymerase sequencing in diarrheal children aged < 5 years in Urumqi, 2012–2014

    图  3  2012-2014年乌鲁木齐市<5岁腹泻儿童诺如病毒衣壳蛋白基因型分布

    Figure  3.  Annual genotype distribution in capsid protein sequencing in diarrheal children aged <5 years in Urumqi, 2012−2014

    表  1  诺如病毒G.4变异株衣壳区构成分布

    Table  1.   Constituent ratio of G.4 variant strains (Capsid region), 2012−2014

    年份 基因型 总计(份)
    GⅡ.4 DenHaag 2006b GⅡ.4 New Orleans GⅡ.4 Sydney 2012
    2012 90.0(18/20) 0 10.0(2/20) 20
    2013 0 0 100(11/11) 11
    2014 0 0 100(17/17) 17
    合计 37.55(18/48) 0 62.5(30/48) 48
      注:括号内数据为阳性数(份)/样本数(份)
    下载: 导出CSV

    表  2  诺如病毒衣壳蛋白区和聚合酶区同时分型结果

    Table  2.   Genotyping results of norovirus in polymerase and capsid region

    基因型 样本数
    (份)
    聚合酶区 衣壳区
    GⅡ.Pe GⅡ.4 Sydney 2012 29
    GⅡ.P4 DenHaag 2006b GⅡ.4 DenHaag 2006b 19
    GⅡ.P7 GⅡ.7 2
    GⅡ.P21 GⅡ.21 2
    GⅡ.P22 GⅡ.22 1
    GⅡ.P17 GⅡ.17 1
    GⅡ.P12 GⅡ.3 E 19
    GⅡ.P12 GⅡ.3 D 7
    GⅡ.P7 GⅡ.6 6
    GⅡ.P7 GⅡ.9 1
    GⅡ. Pe GⅡ.9 1
    GⅡ.P16 GⅡ.13 1
    下载: 导出CSV
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出版历程
  • 收稿日期:  2018-07-25
  • 网络出版日期:  2018-10-29
  • 刊出日期:  2018-10-01

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