单晓英, 白爱英, 刘尊玉. 山东省济南市脑膜炎奈瑟菌多位点序列分型研究[J]. 疾病监测, 2018, 33(12): 1004-1008. DOI: 10.3784/j.issn.1003-9961.2018.12.009
引用本文: 单晓英, 白爱英, 刘尊玉. 山东省济南市脑膜炎奈瑟菌多位点序列分型研究[J]. 疾病监测, 2018, 33(12): 1004-1008. DOI: 10.3784/j.issn.1003-9961.2018.12.009
Xiaoying Shan, Aiying Bai, Zunyu Liu. Multi Locus Sequence Typing of Neisseria meningitidis in Jinan, Shandong[J]. Disease Surveillance, 2018, 33(12): 1004-1008. DOI: 10.3784/j.issn.1003-9961.2018.12.009
Citation: Xiaoying Shan, Aiying Bai, Zunyu Liu. Multi Locus Sequence Typing of Neisseria meningitidis in Jinan, Shandong[J]. Disease Surveillance, 2018, 33(12): 1004-1008. DOI: 10.3784/j.issn.1003-9961.2018.12.009

山东省济南市脑膜炎奈瑟菌多位点序列分型研究

Multi Locus Sequence Typing of Neisseria meningitidis in Jinan, Shandong

  • 摘要:
    目的 分析2005 — 2018年山东省济南市分离的脑膜炎奈瑟菌(Nm)分子流行病学特征。
    方法 采用多位点序列分型(MLST)方法,对Nm的分子分型进行研究,并使用BioNumerics软件进行分子分型分析。
    结果 2005 — 2018年,共分离到130株Nm,涵盖A、B、C、W135、Y 5种血清群及不可分群(NG)。 130株Nm分为37种序列型(ST):2株A群分为2种ST,分别属于已知克隆群(cc)5和未知克隆群(UA);24株B群分为17种ST,分别属于cc4821、cc162及UA;39株C群分为8种ST,分别属于cc4821、cc32及UA;54株W135群分为3种ST,分别属于cc11和cc4821;1株Y群为ST-92型,属于cc92;10株NG分为8种ST,分别属于cc4821、cc198及UA。 108株已知cc菌株中,cc11占48.15%(52/108),cc4821占45.37%(49/108);22株UA菌株包括18种ST,其中,15株B群具有10种ST。
    结论 济南市2005 — 2018年分离的Nm ST型别分布呈现多态性,在NG和B群中尤为明显。 cc4821和cc11为两大优势克隆群,B群Nm呈现多克隆化趋势。

     

    Abstract:
    Objective To study the molecular epidemiology of Neisseria meningitidis isolated in Jinan of Shandong province from 2005 to 2018.
    Methods Multi locus sequence typing (MLST) was used to characterize the N. meningitidis, and the data were analyzed with BioNumerics software.
    Results A total of 130 N. meningitidis isolates were isolated in Jinan from 2005 to 2018, including serogroupA, B, C, W135, Y and non-groupable (NG) strains. The N.meningitidis strains were classified into to 37 sequence types (STs), in which 2 serogroup A strains were classified into 2 STs, belonging to clonal complex 5 and unknown clonal complex; 24 serogroup B strains were classified into 17 STs, belonging to clonal complex 4821, clonal complex 162 and unknown clonal complex; 39 serogroup C strains were classified into 8 STs, belonging to clonal complex 4821, clonal complex 32 and unknown clonal complex; 54 serogroup W135 strains were classified into 3 STs, belonging to clonal complex 11 and clonal complex 4821; 1 serogroup Y strain was ST-92, belonging to clonal complex 92; 10 of NG strains were classified into 8 STs, belonging to clonal complex 4821, clonal complex 198 and unknown clonal complex. Among the 108 strains with known clonal complex, 48.15% (52/108) belonged toclonal complex 11 and 45.37% (49/108) belonged to clonal complex 4821. Twenty-two strains of unknown clonal complex belonged to 18 STs, in which 15 strains were in serogroup B, belonging to 10 STs.
    Conclusion The STs of N. meningitidis in Jinan from 2005 to 2018 showed polymorphism and it was obvious in NG and serogroup B strains. Clonal complex 4821 and clonal complex 11 were two predominant clones. N. meningitidis serogroup B showed polyclonal trend in Jinan.

     

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