北京市房山区首例肠道病毒D68病例临床症状与体征及病原学监测研究

沈玲羽 田竞 张铁钢 龚成 黄芳

沈玲羽, 田竞, 张铁钢, 龚成, 黄芳. 北京市房山区首例肠道病毒D68病例临床症状与体征及病原学监测研究[J]. 疾病监测, 2019, 34(4): 327-331. doi: 10.3784/j.issn.1003-9961.2019.04.011
引用本文: 沈玲羽, 田竞, 张铁钢, 龚成, 黄芳. 北京市房山区首例肠道病毒D68病例临床症状与体征及病原学监测研究[J]. 疾病监测, 2019, 34(4): 327-331. doi: 10.3784/j.issn.1003-9961.2019.04.011
Lingyu Shen, Jing Tian, Tiegang Zhang, Cheng Gong, Fang Huang. Clinical manifestations and etiological characteristics of the first enterovirus-D68 positive case in Fangshan district of Beijing[J]. Disease Surveillance, 2019, 34(4): 327-331. doi: 10.3784/j.issn.1003-9961.2019.04.011
Citation: Lingyu Shen, Jing Tian, Tiegang Zhang, Cheng Gong, Fang Huang. Clinical manifestations and etiological characteristics of the first enterovirus-D68 positive case in Fangshan district of Beijing[J]. Disease Surveillance, 2019, 34(4): 327-331. doi: 10.3784/j.issn.1003-9961.2019.04.011

北京市房山区首例肠道病毒D68病例临床症状与体征及病原学监测研究

doi: 10.3784/j.issn.1003-9961.2019.04.011
基金项目: 北京市科学技术委员会(No. 2017ZX0004206–002);首都医学发展科研基金(No. 2016–2–3011);国家科技重大专项(No. 2017ZX10103004)
详细信息
    作者简介:

    沈玲羽,女,山东省临沂市人,公卫医师,主要从事病原微生物监测及基因组学方面研究,Email:linxiyuai@foxmail.com

    田竞,女,河北省保定市人,公卫医师,主要从事传染病防控及传染病流行病学研究

    通讯作者:

    黄芳,Tel:010–64407032,Email:hhffxdd@126.com

  • 中图分类号: R512.5

Clinical manifestations and etiological characteristics of the first enterovirus-D68 positive case in Fangshan district of Beijing

Funds: This study was supported by the fund from Beijing Municipal Science and Technology Commission (No. 2017ZX0004206–002), Capital Medical Development and Scientific Research Foundation (No. 2016–2–3011), and the fund for National Seience and Technology Major Project of China (No. 2017ZX10103004)
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  • 摘要: 目的了解北京市1例支气管肺炎患者感染肠道病毒D68型(EV-D68)的临床症状与体征及病原学特征。方法收集该患者临床资料,进行病例病程及诊疗分析,采集患者的痰液标本,提取样本的核酸,进行18种常见呼吸道病原体核酸检测,对检测阳性病原体进行VP1区和全基因组测序,并对序列进行遗传进化分析。结果该支气管肺炎患者以高热、咳嗽起病,高热消退后呼吸道症状加重,以喘息和咳嗽为主,抗哮喘药治疗后效果显著。 痰液标本病原学检测为EV-D68阳性,其他17种病原体均为阴性。 基于VP1区构建的系统进化树显示为EV-D68 B3基因亚型,全基因组同源性分析显示同源性最高的是2015年日本株(98.72% nt, 99.95% aa)、2015年香港株(98.61% nt,99.86% aa)和2016年美国株(98.24% nt,99.86% aa),与北京市报道的EV-D68同源性最高的是2014年“2014-R132”株(96.89% nt,99.45% aa)。结论EV-D68是引起支气管肺炎的重要病原体,应该加强EV-D68的病原学监测,为临床诊疗及疾病控制提供基础数据。
  • 图  1  北京市房山区某患者人感染EV-D68感染、发病及诊疗过程

    Figure  1.  Onset and diagnosis/treatment of patient infected with EV-D68

    图  2  基于VP1区的肠道病毒D68亚型分析

    Figure  2.  Phylogenetic tree of EV-D68 strains based on VP1 gene region

    图  3  肠道病毒D68全基因组系统进化树

    注:●. 本研究毒株;▲. 与本研究同源性最高的国外毒株;▼. 与本研究同源性最高的国内毒株

    Figure  3.  Phylogenetic tree of EV-D68 strains based on complete genome sequences

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出版历程
  • 收稿日期:  2018-11-06
  • 网络出版日期:  2019-04-22
  • 刊出日期:  2019-04-01

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