2017年安徽省马鞍山市环境来源气单胞菌的分子特征和耐药分析

汪永禄 黄振洲 王利 王蓉 代航 蔡红艳 杜小莉 王多春

汪永禄, 黄振洲, 王利, 王蓉, 代航, 蔡红艳, 杜小莉, 王多春. 2017年安徽省马鞍山市环境来源气单胞菌的分子特征和耐药分析[J]. 疾病监测, 2019, 34(9): 805-810. doi: 10.3784/j.issn.1003-9961.2019.09.008
引用本文: 汪永禄, 黄振洲, 王利, 王蓉, 代航, 蔡红艳, 杜小莉, 王多春. 2017年安徽省马鞍山市环境来源气单胞菌的分子特征和耐药分析[J]. 疾病监测, 2019, 34(9): 805-810. doi: 10.3784/j.issn.1003-9961.2019.09.008
Yonglu Wang, Zhenzhou Huang, Li Wang, Rong Wang, Hang Dai, Hongyan Cai, Xiaoli Du, Duochun Wang. Molecular characteristics and drug resistance of Aeromonas from environmental Sources in Ma'anshan, Anhui[J]. Disease Surveillance, 2019, 34(9): 805-810. doi: 10.3784/j.issn.1003-9961.2019.09.008
Citation: Yonglu Wang, Zhenzhou Huang, Li Wang, Rong Wang, Hang Dai, Hongyan Cai, Xiaoli Du, Duochun Wang. Molecular characteristics and drug resistance of Aeromonas from environmental Sources in Ma'anshan, Anhui[J]. Disease Surveillance, 2019, 34(9): 805-810. doi: 10.3784/j.issn.1003-9961.2019.09.008

2017年安徽省马鞍山市环境来源气单胞菌的分子特征和耐药分析

doi: 10.3784/j.issn.1003-9961.2019.09.008
基金项目: 马鞍山市卫计委科教专项资金(No.2016-19)
详细信息
    作者简介:

    汪永禄,男,安徽省马鞍山市人,副主任技师,主要从事病原菌检测相关工作,Email:mas_wyl@163.com

    通讯作者:

    王多春,Tel:010–58900744,Email:wangduochun@icdc.cn

  • 中图分类号: R378

Molecular characteristics and drug resistance of Aeromonas from environmental Sources in Ma'anshan, Anhui

Funds: This study was supported by the Special Fund for Science and Education of Health and Family Planning Commission of Maanshan City (No.2016-19)
More Information
  • 摘要: 目的了解2017年安徽省马鞍山市环境来源气单胞菌的流行特征、毒力基因特征及耐药状况。方法2017年对来自马鞍山市监测的海水产品、包装食品、生活用水、游泳池水样、食物中毒样本等进行气单胞菌分离检测,采用管家基因rpoD序列分析进行种水平鉴定,PCR检测气单胞菌6种毒力基因(FlaElastasehlyAastactascF-G)、脉冲场凝胶电泳(PFGE)分型分析分子型别,药敏试验检测药物敏感性。结果共检测样品869份,气单胞菌检出率为7.6%(66/869),检出率最高的是农村生活用水、食物中毒样品和海水产品。 66株气单胞菌分为7个种,最多的种是维氏气单胞菌(36.4%,24/869)、嗜水气单胞菌(19.7%,13/869)和简氏气单胞菌(19.7%,13/869)。 64株气单胞菌携带至少1种毒力基因,Flaact总检出率最高(53.1%),Fla在豚鼠气单胞菌中检出率最高(83.3%),act在嗜水气单胞菌检出率最高(69.2%)。 66株气单胞菌分为64个PFGE带型,表现为多样性。 药敏实验显示,气单胞菌对头孢替坦等7种抗生素100%敏感,对头孢唑啉等5种抗生素部分敏感(耐药率1.5% ~ 21.2%),而对氨苄西林、氨苄西林/舒巴坦的耐药率均高达90.0%。结论2017年马鞍山地区气单胞菌污染较为严重,携带较高比例的多种毒力基因,并且对多种抗生素耐药性较高,应该加强对气单胞菌及其耐药性的监测。
  • 图  1  气单胞菌种水平rpoD序列鉴定结果

    注:黑色三角形代表该种单胞菌的集合,三角形的大小反映为菌株数量的多少,括号内为菌株数量

    Figure  1.  Identification of Aeromonas strains at species level based on rpoD sequences.

    图  2  64株气单胞菌脉冲场凝胶电泳分型结果

    Figure  2.  PFGE results of 64 Aeromonas strains

    表  1  气单胞菌毒力基因检测引物

    Table  1.   Primers used for detection of virulence genes of Aeromonas

    毒力
    基因
    GenBank
    序列号
    引物序列(5′ ~ 3′)产物长
    度(bp)
    FlaFlaFTCCAACCGTYTGACCTC608
    FlaRGMYTGGTTGCRATGGT
    ElastaseElaFACACGGTCAAGGAGATCAAC513
    ElaRCGCTGGTGTTGGCCAGCAGG
    hlyAhlyAFGGCCGGTGGCCCGAAGATACGGG597
    hlyARGGCGGCGCCGGACGAGACGGG
    astastFTCTCCATGCTTCCCTTCCACT260
    astRGTGTAGGGATTGAAGAAGCCG
    actactFAGAAGGTGACCACCAAGAACA232
    actRAACTGACATCGGCCTTGAACTC
    Ascf-GASCF-GFATGAGGTCATCTGCTCGCGC900
    ASCF-GRGGAGCACAACCATGGCTGAT
    下载: 导出CSV

    表  2  不同样本中气单胞菌检出情况

    Table  2.   Detection of Aeromonas in different samples

     样品名称样品数(份)分离阳性数(份)阳性率(%)
    农村生活用水1273527.6
    食物中毒样品 821012.2
    海水产品100 9 9.0
    游泳池水121 7 5.8
    城区生活用水278 5 1.8
    包装食品161 0 0.0
      合计86966 7.6
    下载: 导出CSV

    表  3  不同样品中气单胞菌的种类分布

    Table  3.   Species distribution of Aeromonas in different samples

     样品名称维氏气单胞菌(株)嗜水气单胞菌(株)简氏气单胞菌(株)水生气单胞菌(株)豚鼠气单胞菌(株)疱氏气单胞菌(株)舒氏气单胞菌(株)总计(株)
    农村生活用水18 4 9300135
    食物中毒样品 3 1 2040010
    海水产品 0 2 05200 9
    游泳池水 1 5 10000 7
    城区生活用水 2 1 10010 5
      合计241313861166
    下载: 导出CSV

    表  4  66株气单胞菌毒力基因检测情况

    Table  4.   Detections of virulence genes of Aeromonas strains

    毒力
    基因
    维氏气单胞菌(n=24)嗜水气单胞菌(n=13)简氏气单胞菌(n=13)水生气单胞菌(n=8)豚鼠气单胞菌(n=6)合计
    检出数(株)检出率(%)检出数(株)检出率(%)检出数(株)检出率(%)检出数(株)检出率(%)检出数(株)检出率(%)检出数(株)检出率(%)
    Fla1354.2646.2538.5562.5583.33453.1
    act1250.0969.2646.2337.5466.73453.1
    Elastase 625.0861.5753.8450.0350.02843.8
    hlyA 833.3646.2430.8450.0233.32437.5
    Ast 937.5646.3430.8337.5350.02335.9
    Ascf-G 625.0646.4323.1225.0233.31828.1
    下载: 导出CSV

    表  5  66株气单胞菌菌的药敏结果情况

    Table  5.   Drug susceptibility results of Aeromonas strains

     药物名称敏感中度敏感耐药
    菌株数量(株)敏感率(%)菌株数量(株)敏感率(%)菌株数量(株)耐药率(%)
    氨苄西林 1 1.5 3 4.56294.0
    氨苄西林/舒巴坦 3 4.5 3 4.56090.9
    头孢唑啉33 50.01827.31522.7
    头孢替坦66100.0 0 0.0 0 0.0
    头孢他啶64 97.0 0 0.0 2 3.0
    头孢曲松65 98.5 0 0.0 1 1.5
    氨曲南64 96.7 0 0.0 2 3.3
    亚胺培南66100.0 0 0.0 0 0.0
    阿米卡星65 98.5 1 1.5 0 0.0
    庆大霉素66100.0 0 0.0 0 0.0
    妥布霉素65 98.5 1 1.5 0 0.0
    环丙沙星66100.0 0 0.0 0 0.0
    左旋氧氟沙星66100.0 0 0.0 0 0.0
    呋喃妥因66100.0 0 0.0 0 0.0
    甲氨苄啶/磺胺甲恶唑65 98.5 0 0.0 1 1.5
    哌拉西林/他唑巴坦66100.0 0 0.0 0 0.0
    下载: 导出CSV
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  • 收稿日期:  2019-05-06
  • 网络出版日期:  2019-08-13
  • 刊出日期:  2019-09-01

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