2016年山东省聊城市临床病例中2种罕见施万菌的鉴定

梁胜楠 姜祥坤 杜银菊 周璞 刘莉 程利红 王多春

梁胜楠, 姜祥坤, 杜银菊, 周璞, 刘莉, 程利红, 王多春. 2016年山东省聊城市临床病例中2种罕见施万菌的鉴定[J]. 疾病监测, 2019, 34(9): 817-821. doi: 10.3784/j.issn.1003-9961.2019.09.010
引用本文: 梁胜楠, 姜祥坤, 杜银菊, 周璞, 刘莉, 程利红, 王多春. 2016年山东省聊城市临床病例中2种罕见施万菌的鉴定[J]. 疾病监测, 2019, 34(9): 817-821. doi: 10.3784/j.issn.1003-9961.2019.09.010
Shengnan Liang, Xiangkun Jiang, Yinju Du, Pu Zhou, Li Liu, Lihong Cheng, Duochun Wang. Isolation of two rare Shewanella spp. from specimens of patients in Liaocheng in 2016[J]. Disease Surveillance, 2019, 34(9): 817-821. doi: 10.3784/j.issn.1003-9961.2019.09.010
Citation: Shengnan Liang, Xiangkun Jiang, Yinju Du, Pu Zhou, Li Liu, Lihong Cheng, Duochun Wang. Isolation of two rare Shewanella spp. from specimens of patients in Liaocheng in 2016[J]. Disease Surveillance, 2019, 34(9): 817-821. doi: 10.3784/j.issn.1003-9961.2019.09.010

2016年山东省聊城市临床病例中2种罕见施万菌的鉴定

doi: 10.3784/j.issn.1003-9961.2019.09.010
基金项目: 山东医药卫生科技发展计划(No.2016WS0201)
详细信息
    作者简介:

    梁胜楠,女,山东省聊城市人,硕士,主管技师,从事病原微生物检验和研究工作,Email:lccdclsn@126.com

    通讯作者:

    王多春,Tel: 010–58900744,Email: wangduochun@icdc.cn

  • 中图分类号: R379.9

Isolation of two rare Shewanella spp. from specimens of patients in Liaocheng in 2016

Funds: This study was supported by the Medical Health Science and Technology Development Project of Shandong Province (No.2016WS0201)
More Information
  • 摘要: 目的对2016年山东省聊城市临床患者分离株进行鉴定以及分析其生化特性和药物敏感性。方法对患者来源标本及6株菌株进行选择性培养基分离培养,并进行生化试验、溶血试验、耐盐耐热实验和药敏试验,使用API生化鉴定系统、VITEK 2 Compact、全自动快速微生物质谱检测系统进行鉴定,并进行16S rRNA和gyrB序列分析。结果8株菌在菌落形态和生化特性上差异无统计学意义,只有溶血状态有所不同,临床鉴定系统有局限性,只能分辨到海藻施万菌和腐败施万菌,进一步使用16S rRNA和gyrB序列检测方法,确认患者分离株属于2种罕见的施万菌,即鲍施万菌和优佩内施万菌。结论首次在中国大陆的临床病例中,鉴定了2株由罕见施万菌导致的感染病例。 由施万菌属引起的临床感染还需要进一步重视,并加强监测。
  • 图  1  16S rRNA(A)和gyrB (B)序列进化树鉴定结果

    注:黑体显示的是从本研究分离的2株施万菌;括号内为参考菌株的16S rRNA和gyrB序列在 GenBank中的编号

    Figure  1.  Phylogenic tree identification results of 16S rRNA(A)and gyrB (B) sequences

    表  1  微生物鉴定系统结果

    Table  1.   Result of microbiological identification system

    菌株编号API 20EVITEK 2VITEK MS
    鉴定结果ID值(%)t鉴定结果可信度(%)鉴定结果可信度(%)
    20-23RT腐败施万菌99.700.90腐败施万菌99.00腐败施万菌99.90
    ATCC 51192T腐败施万菌99.700.90海藻施万菌99.00海藻施万菌99.90
    DW01 T腐败施万菌99.700.90腐败施万菌99.00海藻施万菌99.90
    MAS2723腐败施万菌99.000.65腐败施万菌99.00海藻施万菌99.90
    LC2016-5腐败施万菌99.000.90腐败施万菌99.00腐败施万菌99.90
    2014LZ6-2腐败施万菌99.900.72腐败施万菌99.00腐败施万菌99.90
    2014LZ7-8腐败施万菌99.900.72腐败施万菌99.00腐败施万菌99.90
    LC2016-1腐败施万菌99.000.82海藻施万菌99.00海藻施万菌99.90
    下载: 导出CSV

    表  2  药敏试验结果

    Table  2.   Result of antibiotic susceptibility test

    抗生素菌株编号
    20-23RTATCC 51192TDW01TMAS2723LC2016-52014LZ6-22014LZ7-8LC2016-1
    氨苄西林4(S)16(I)8(S)≤2(S)64(R)4(S)≤2(S)4(S)
    头孢他啶≤1(S)2(S)≤1(S)≤1(S)≤1(S)≤1(S)≤1(S)≤1(S)
    氨苄西林/舒巴坦4/2(S)64/32(R)32/16(R)≤2/1(S)16/8(I)≤2/1(S)≤2/1(S)≤2/1(S)
    亚胺培南1(S)1(S)1(S)≤0.25(S)1(S)0.5(S)≤0.25(S)0.5(S)
    四环素≤1(S)4(S)≤1(S)≤1(S)≤1(S)≤1(S)≤1(S)≤1(S)
    萘啶酸≤2(S)≤2(S)≤2(S)≤2(S)>64(R)≤2(S)≤2(S)>64(R)
    红霉素>16(R)1(S)≤0.5(S)≤0.5(S)≤0.5(S)≤0.5(S)≤0.5(S)≤0.5(S)
    头孢西丁64(R)64(R)32(R)≤2(S)4(S)≤2(S)≤2(S)≤2(S)
    氯霉素≤2(S)4(S)≤2(S)≤2(S)≤2(S)≤2(S)≤2(S)32(R)
    头孢噻肟≤0.25(S)2(I)≤0.25(I)≤0.25(S)≤0.25(S)≤0.25(S)≤0.25(S)≤0.25(S)
    头孢唑林>16(R)>16(R)>16(R)8(R)>16(R)16(R)16(R)2(S)
    庆大霉素≤1(S)≤1(S)≤1(S)≤1(S)≤1(S)≤1(S)≤1(S)≤1(S)
    复方磺胺≤0.25/0.475(S)≤0.25/0.475(S)≤0.25/0.475(S)≤0.25/0.475(S)≤0.25/0.475(S)≤0.25/0.475(S)≤0.25/0.475(S)>8/152(R)
    阿奇霉素>64(S)>64(S)>64(S)>64(S)>64(S)>64(S)>64(S)>64(S)
    环丙沙星0.125(I)0.25(I)0.25(I)≤0.03(S)4(R)0.125(I)≤0.03(S)8(R)
      注:表内数据为最低抑菌浓度(μg/ml);R. 耐药;I. 中等;S. 敏感
    下载: 导出CSV
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  • 2019-0192 从聊城市患者标本中分离两株罕见施万菌,腹泻病原监测组稿.docx
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  • 收稿日期:  2019-05-28
  • 网络出版日期:  2019-08-13
  • 刊出日期:  2019-09-01

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