Surveillance results of avian influenza virus in external environment in Quzhou, Zhejiang, 2013–2018
-
摘要:
目的了解浙江省衢州地区外环境禽流感病毒流行特征，为该地区人禽流感疫情的防控和预测预警提供科学依据。 方法采集2013 — 2018年衢州地区外环境禽流感监测标本，采用实时荧光反转录–聚合酶链式反应方法对采集的外环境标本进行禽流感病毒核酸检测，并进行统计学分析。 结果2013 — 2018年共采集禽流感外环境标本5 290份，检出流感病毒A型（FluA）阳性标本1 809份，阳性率为34.20%；其中以H9亚型和A型未分型为主，分别占44.50%和29.13%。 开化县检出阳性率最高（54.85%），龙游县最低（17.25%）。 5类场所中，城乡活禽市场检出阳性率最高（45.94%）。 6种类型标本中，宰杀或摆放禽肉案板表面的擦拭标本检出阳性率最高（58.96%）。第一季度（38.06%）和第四季度（37.23%）检出阳性率较高。 不同时间、地区、场所、标本类型的标本阳性检出率差异均具有统计学意义（P＜0.001）。 结论衢州地区外环境中存在多种亚型及混合型禽流感病毒，应加强外环境禽流感监测力度，同时采取有效的防控措施降低人群感染的风险。 -
关键词:
- 禽流感病毒 /
- 外环境 /
- 监测 /
- 实时荧光反转录–聚合酶链式反应
Abstract:ObjectiveTo understand the distribution of avian influenza virus in the external environment in Quzhou, Zhejiang province, from 2013 to 2018, and provide scientific evidence for the prevention and control of human infection with avian influenza virus. MethodsThe external environmental samples were collected from different surveillance sites of Quzhou for the nucleic acid detection of avian influenza viruses by using real-time RT-PCR during 2013–2018, and the results were used for statistical analysis. ResultsA total of 5 290 environmental samples were collected from 2013 to 2018, in which 1 809 were positive for avian influenza A virus (34.20%), The subtype identification showed that H9 and unspecific type were main subtypes, the positive rate were 44.50% and 29.13% respectively. The positive rate was highest in Kaihua county (54.85%) and lowest in Longyou county (17.25%). The detection rate in live poultry markets was highest (45.94%) in 5 types of places, and the detection rate in surface swabs of chopping boards for slaughtering or placing poultry products was highest (58.96%) in 6 types of samples. The positive rates were significantly higher during January-March (38.06%) and during October-December (37.23%). There were significant differences in time, area, place and sample type specific distributions of avian influenza viruses (all P＜0.001). ConclusionThere were different subtypes and mixed types of avian influenza virus contamination in the environment in Quzhou. It is important to strengthen the surveillance for avian influenza virus in environment and take comprehensive prevention and control measures to reduce the risk of human infection with avian influenza virus. -
Key words:
- Avian influenza virus /
- External environment /
- Surveillance /
- Real-time RT-PCR
-
图 1 2013－2018年衢州地区外环境禽流感病毒检出率及型别构成
Figure 1. Detection rate and subtype constituent ratio of avian influenza viruses in external environment in Quzhou, 2013–2018
表 1 2013－2018年衢州市不同地区外环境标本禽流感病毒监测结果
Table 1. Surveillance results of avian influenza virus in external environment in different areas in Quzhou, 2013–2018
监测地区 检测数
（份）禽流感病毒A型 不同亚型检出数（份） A型未分型 阳性数（份） 阳性率（%） H5 H7 H9 H5+H7 H5+H9 H7+H9 H5+H7+H9 柯城区 1 129 341 30.20 12 24 179 0 6 33 2 85 衢江区 811 297 36.62 1 23 151 4 7 36 4 71 江山市 843 334 39.62 41 3 143 0 53 12 2 80 开化县 855 469 54.85 28 55 233 10 16 16 14 97 龙游县 765 132 17.25 4 30 36 0 3 1 0 58 常山县 887 236 26.61 6 17 63 0 5 8 1 136 合计 5 290 1 809 34.20 92 152 805 14 90 106 23 527 
下载: 导出CSV
表 2 2013－2018年衢州地区不同场所禽流感病毒监测结果
Table 2. Surveillance results of avian influenza virus in different sites in Quzhou, 2013–2018
监测场所 检测数
（份）禽流感病毒A型 不同亚型检出数（份） A型未分型 阳性数（份） 阳性率（%） H5 H7 H9 H5+H7 H5+H9 H7+H9 H5+H7+H9 城乡活禽市场 3 731 1 714 45.94 91 145 765 14 89 96 23 491 家禽规模养殖场（户） 386 17 4.40 0 1 1 0 0 7 0 8 家禽散养户集中的地区 948 28 2.95 0 0 14 0 0 1 0 13 家禽屠宰加工厂 75 32 42.67 1 4 22 0 1 1 0 3 其他 150 18 12.00 0 2 3 0 0 1 0 12 合计 5 290 1 809 34.20 92 152 805 14 90 106 23 527
下载: 导出CSV
表 3 2013－2018年衢州地区不同类型标本禽流感病毒监测结果
Table 3. Surveillance results of avian influenza virus in different types of samples in Quzhou, 2013–2018
标本类型 检测数
（份）禽流感病毒A型 不同亚型检出数（份） A型未分型 阳性数（份） 阳性率（%） H5 H7 H9 H5+H7 H5+H9 H7+H9 H5+H7+H9 宰杀或摆放禽肉案板表面的擦拭 753 444 58.96 38 56 189 7 32 16 5 101 笼具表面擦拭 770 265 34.42 5 29 92 2 9 24 2 102 粪便 1 906 436 22.88 12 29 202 1 8 22 2 160 禽类饮水 504 126 25.00 7 6 67 0 7 7 8 24 清洗禽类的污水 737 335 45.45 20 21 155 4 23 25 4 83 其他 620 203 32.74 10 11 100 0 11 12 2 57 合计 5 290 1 809 34.20 92 152 805 14 90 106 23 527
下载: 导出CSV
表 4 2013－2018年衢州地区不同季节禽流感病毒监测结果
Table 4. Surveillance results of avian influenza virus in different seasons in Quzhou, 2013–2018
季度 检测数
（份）禽流感病毒A型 不同亚型检出数（份） A型未分型 阳性数（份） 阳性率（%） H5 H7 H9 H5+H7 H5+H9 H7+H9 H5+H7+H9 第一季度 2 307 878 38.06 55 85 304 11 77 59 22 265 第二季度 532 115 21.62 6 5 53 0 0 12 0 39 第三季度 474 80 16.88 2 1 58 0 0 0 0 19 第四季度 1 977 736 37.23 29 61 390 3 13 35 1 204 合计 5 290 1 809 34.20 92 152 805 14 90 106 23 527
下载: 导出CSV
-
[1] Perroncito E. Epizoozia tifoide nei gallinacei[J]. Annali Accad Agri Torino, 1878,21:87–126. [2] Li CJ, Yu KZ, Tian GB, et al. Evolution of H9N2 influenza viruses from domestic poultry in Mainland China[J]. Virology, 2005,340(1):70–83. DOI: 10.1016/j.virol.2005.06.025. [3] Sun YP, Pu J, Jiang ZL, et al. Genotypic evolution and antigenic drift of H9N2 influenza viruses in China from 1994 to 2008[J]. Vet Microbiol, 2010,146(3/4):215–225. DOI: 10.1016/j.vetmic.2010.05.010. [4] Kageyama T, Fujisaki S, Takashita E, et al. Genetic analysis of novel avian A(H7N9) influenza viruses isolated from patients in China, February to April 2013[J]. Euro Surveill, 2013,18(15):20453. [5] Gao RB, Cao B, Hu YW, et al. Human infection with a novel avian-origin influenza A (H7N9) virus[J]. N Engl J Med, 2013,368(20):1888–1897. DOI: 10.1056/NEJMoa1304459. [6] 张人杰, 李娜, 王笑笑, 等. 2013-2016年浙江省人感染H7N9禽流感疫情的空间分析及回归模型构建[J]. 疾病监测,2019,34(1):15–20. DOI: 10.3784/j.issn.1003−9961.2019.01.006.Zhang RJ, Li N, Wang XX, et al. Spatial analysis and mathematical modeling of human infection with avian influenza A(H7N9) virus in Zhejiang, 2013–2016[J]. Dis Surveill, 2019,34(1):15–20. DOI: 10.3784/j.issn.1003−9961.2019.01.006. [7] 易槐明, 陈恩富, 樊海笑, 等. 常山县1例同病房密切接触者确诊人感染H7N9禽流感的调查报告[J]. 中国农村卫生事业管理,2016,36(9):1165–1167.Yi HM, Chen EF, Fan HX, et al. Human infection with H7N9 avian influenza via close contact with wardmates in Changshan: a case study[J]. Chin Rural Health Ser Admin, 2016,36(9):1165–1167. [8] 程伟, 王笑笑, 刘社兰, 等. 浙江省三波人感染H7N9禽流感疫情流行特征与趋势分析[J]. 中国预防医学杂志,2016,17(4):246–250. DOI: 10.16506/j.1009−6639.2016.04.002.Cheng W, Wang XX, Liu SL, et al. Epidemiological characteristics and trend analysis of human infection with avian influenza A(H7N9) in Zhejiang[J]. Chin Prev Med, 2016,17(4):246–250. DOI: 10.16506/j.1009−6639.2016.04.002. [9] 王笑笑, 程伟, 余昭, 等. 浙江省冬春季涉禽场所禽流感病毒污染特征及影响因素研究[J]. 中华预防医学杂志,2016,50(3):250–254. DOI: 10.3760/cma.j.issn.0253−9624.2016.03.012.Wang XX, Cheng W, Yu Z, et al. Avian influenza virus in various environments and risk factors for the contamination of live poultry markets during winter and spring season in Zhejiang province[J]. Chin J Prev Med, 2016,50(3):250–254. DOI: 10.3760/cma.j.issn.0253−9624.2016.03.012. [10] Chen EF, Wang FJ, Lv HK, et al. The first avian influenza A (H7N9) viral infection in humans in Zhejiang province, China: a death report[J]. Front Med, 2013,7(3):333–344. DOI: 10.1007/s11684−013−0275−1. [11] 丁华, 谢立, 考庆君, 等. 杭州市首例人感染H7N9禽流感病例调查[J]. 中华预防医学杂志,2013,47(12):1155–1156. DOI: 10.3760/cma.j.issn.0253−9624.2013.12.019.Ding H, Xie L, Kao QJ, et al. Epidemiological investigation of the first case of human infection with H7N9 avian influenza in Hangzhou[J]. Chin J Prev Med, 2013,47(12):1155–1156. DOI: 10.3760/cma.j.issn.0253−9624.2013.12.019. [12] Peiris JSM, Cowling BJ, Wu JT, et al. Interventions to reduce zoonotic and pandemic risks from avian influenza in Asia[J]. Lancet Infect Dis, 2016,16(2):252–258. DOI: 10.1016/S1473−3099(15)00502−2. [13] 张烨, 李晓丹, 邹淑梅, 等. 2009-2013年我国活禽市场环境样本中禽流感病毒的检测[J]. 病毒学报,2015,31(6):615–619.Zhang Y, Li XD, Zou SM, et al. Detection of avian influenza virus in environmental samples collected from live poultry markets in china during 2009–2013[J]. Chin J Virol, 2015,31(6):615–619. [14] Wang XX, Wang QM, Cheng W, et al. Risk factors for avian influenza virus contamination of live poultry markets in Zhejiang, China during the 2015–2016 human influenza season[J]. Sci Rep, 2017,7:42722. DOI: 10.1038/srep42722. [15] 李保娣, 李红育, 张慧, 等. 甘肃省2015-2016年外环境禽流感监测及职业暴露人群血清学分析[J]. 中国病毒病杂志,2017,7(4):267–270. DOI: 10.16505/j.2095−0136.2017.04.006.Li BD, Li HY, Zhang H, et al. Avian influenza virus contaminations in environment and occupational exposure in 2015 and 2016 in Gansu province of China[J]. Chin J Viral Dis, 2017,7(4):267–270. DOI: 10.16505/j.2095−0136.2017.04.006. [16] 刘琳琳, 叶国军, 江永忠, 等. 2013-2014年湖北省外环境中禽流感病毒污染情况分析[J]. 微生物与感染,2016,11(3):159–164.Liu LL, Ye GJ, Jiang YZ, et al. Avian influenza viruses in environmental samples collected in Hubei province, 2013–2014[J]. J Microbes Infect, 2016,11(3):159–164. -
2019-0011 2013-2018年衢州地区外环境禽流感病毒监测分析.docx
-
点击查看大图
图(1) / 表(4)
计量
- 文章访问数: 10627
- HTML全文浏览量: 3279
- PDF下载量: 34
- 被引次数: 0
