何飞, 聂富, 张雅婷, 吕静, 杨红梅, 李国明, 朱兵清, 邵祝军. 湖北省随州市健康人群嗜血杆菌带菌率及其药物敏感性分析[J]. 疾病监测, 2019, 34(11): 1031-1035. DOI: 10.3784/j.issn.1003-9961.2019.11.017
引用本文: 何飞, 聂富, 张雅婷, 吕静, 杨红梅, 李国明, 朱兵清, 邵祝军. 湖北省随州市健康人群嗜血杆菌带菌率及其药物敏感性分析[J]. 疾病监测, 2019, 34(11): 1031-1035. DOI: 10.3784/j.issn.1003-9961.2019.11.017
Fei He, Fu Nie, Yating Zhang, Jing Lyu, Hongmei Yang, Guoming Li, Bingqing Zhu, Zhujun Shao. Investigation of Haemophilus and its antibiotic susceptibility analysis in Suizhou, Hubei[J]. Disease Surveillance, 2019, 34(11): 1031-1035. DOI: 10.3784/j.issn.1003-9961.2019.11.017
Citation: Fei He, Fu Nie, Yating Zhang, Jing Lyu, Hongmei Yang, Guoming Li, Bingqing Zhu, Zhujun Shao. Investigation of Haemophilus and its antibiotic susceptibility analysis in Suizhou, Hubei[J]. Disease Surveillance, 2019, 34(11): 1031-1035. DOI: 10.3784/j.issn.1003-9961.2019.11.017

湖北省随州市健康人群嗜血杆菌带菌率及其药物敏感性分析

Investigation of Haemophilus and its antibiotic susceptibility analysis in Suizhou, Hubei

  • 摘要:
    目的了解湖北省随州市健康人群嗜血杆菌的带菌率和抗生素敏感性。
    方法分别于2011年和2016年采集湖北省随州市健康人群咽拭子,进行嗜血杆菌的分离培养,并采用玻片凝集和荧光PCR方法对流感嗜血杆菌(Hi)进行血清型和基因型鉴定。 采用K-B纸片扩散法对所有菌株进行体外药物敏感性试验。
    结果2011年和2016年湖北省随州市Hi、溶血嗜血杆菌(Hh)和副流感嗜血杆菌(Hp)的阳性检出率分别为11.90%、4.76%、2.86%和14.76%、13.81%、3.33%。 Hi对氨苄西林、头孢克洛、头孢克肟、阿奇霉素、阿莫西林/克拉维酸、环丙沙星和美罗培南的敏感率分别为35.71%、57.14%、83.93%、83.93%、87.50%、96.43%、96.43%,Hh的敏感率为41.03%、56.41%、94.87%、94.87%、76.92%、97.44%、92.31%,Hp 的敏感率为53.85%、76.92%、84.62%、100%、76.92%、53.85%、76.92%。 仅HiHp对美罗培南(P=0.015)和环丙沙星(P<0.001)敏感性差异有统计学意义,其他差异均无统计学意义(P>0.050)。 与2011年相比,2016年Hi对氨苄西林(P=0.025)、头孢克洛(P=0.044)、头孢克肟(P=0.027)和阿奇霉素(P=0.003)敏感性下降,差异具有统计学意义;Hh仅对阿莫西林/克拉维酸(P=0.045)和阿奇霉素(P=0.013)敏感性下降,差异具有统计学意义;Hp对7种抗生素差异均无统计学意义。
    结论不同嗜血杆菌对抗生素的敏感性存在差异。 随着时间推移,嗜血杆菌对部分抗生素的敏感性明显降低。

     

    Abstract:
    ObjectiveTo investigative the antibiotic susceptibility of common Haemophilus in healthy population in Suizhou of Hubei province.
    MethodsIn 2011 and 2016, the throat swabs of healthy population in Suizhou were collected for Haemophilus isolation and culture, slide agglutination test and fluorescence PCR were conducted to identify the serotype and genotype of Haemophilus. Disk diffusion method (K-B method) was used to detect the antibiotic susceptibility of Haemophilus in vitro.
    ResultThe positive detection rates of Haemophilus, Hemophilus hemolyticus and Haemophilus parainfluenzae in 2011 and 2016 were 11.90%, 4.76%,2.86% and 14.76%, 13.81%, 3.33%, respectively. The sensitivity rates of Haemophilus to ampicillin, cefaclor, cefixime, azithromycin, amoxicillin/clavulanic acid, ciprofloxacin and meropenem were 35.71%, 57.14%, 83.93%, 83.93%, 87.50%, 96.43% and 96.43% respectively; the sensitivity rates of H. hemolyticus to these antibiotics were 41.03%, 56.41%, 94.87%, 94.87%, 76.92%, 97.44% and 92.31% respectively and the sensitivity rates of H. parainfluenzae to these antibiotics were 53.85%, 76.92%, 84.62%, 100%, 76.92%, 53.85% and 76.92%, respectively. The sensitivity rates of Haemophilus and H. parainfluenzae to meropenem (P=0.015) and ciprofloxacin (P<0.001) showed significant differences. However, the sensitivity rates to other five antibiotics exhibited no significant differences (P>0.05). Compared with the Haemophilus isolated in 2011, the isolates of 2016 showed an obvious decrease in sensitivity to ampicillin (P=0.025), cefaclor (P=0.044), cefixime (P=0.027) and azithromycin (P=0.003). The sensitivity to amoxicillin/clavulanic acid (P=0.045) and azithromycin (P=0.013) of H. hemolyticus showed significant decreases. The sensitivity rates of H. parainfluenzae to the 7 antibiotics showed no significant differences.
    ConclusionThe sensitivity of Haemophilus varied with its species. Over time, the sensitivity of Haemophilus showed obvious decreases to some antibiotics.

     

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