高级检索
李云逸, 陆菁, 杨玉颖, 费洁, 张莉萍, 李智, 胡家瑜, 李崇山. 2015－2018年上海市环境污水中非脊髓灰质炎肠道病毒的分布及柯萨奇病毒B5的分子特征[J]. 疾病监测, 2019, 34(12): 1089-1095. DOI: 10.3784/j.issn.1003-9961.2019.12.012
引用本文: 李云逸, 陆菁, 杨玉颖, 费洁, 张莉萍, 李智, 胡家瑜, 李崇山. 2015－2018年上海市环境污水中非脊髓灰质炎肠道病毒的分布及柯萨奇病毒B5的分子特征[J]. 疾病监测, 2019, 34(12): 1089-1095. DOI: 10.3784/j.issn.1003-9961.2019.12.012
shu
Yunyi Li, Jing Lu, Yuying Yang, Jie Fei, Liping Zhang, Zhi Li, Jiayu Hu, Chongshan Li. Distribution of non-polio enteroviruses and molecular characteristics of coxsackievirus B5 isolated from environmental sewage in Shanghai, 2015–2018[J]. Disease Surveillance, 2019, 34(12): 1089-1095. DOI: 10.3784/j.issn.1003-9961.2019.12.012
Citation: Yunyi Li, Jing Lu, Yuying Yang, Jie Fei, Liping Zhang, Zhi Li, Jiayu Hu, Chongshan Li. Distribution of non-polio enteroviruses and molecular characteristics of coxsackievirus B5 isolated from environmental sewage in Shanghai, 2015–2018[J]. Disease Surveillance, 2019, 34(12): 1089-1095. DOI: 10.3784/j.issn.1003-9961.2019.12.012
shu

2015－2018年上海市环境污水中非脊髓灰质炎肠道病毒的分布及柯萨奇病毒B5的分子特征

Distribution of non-polio enteroviruses and molecular characteristics of coxsackievirus B5 isolated from environmental sewage in Shanghai, 2015–2018

    摘要
  • 摘要:
    目的分析2015 — 2018年上海市闵行区和嘉定区环境污水监测中非脊髓灰质炎肠道病毒（NPEV）的分布特征，探讨环境污水、健康儿童和急性弛缓性麻痹（AFP）病例监测中柯萨奇病毒B组5型（Cox B5）分离株之间的关系和变迁规律。
    方法选择上海市闵行区和嘉定区污水处理厂作为长期固定监测点，每月采集监测点的污水进行浓缩富集，取上清液并接种于人横纹肌肉瘤细胞（RD）和人喉癌上皮细胞（Hep-2）进行病毒分离。通过反转录–聚合酶链式反应（RT-PCR）获得VP1区序列并进行分析。
    结果2015 — 2018年共采集污水样本84份，共分离242株NPEV，包含19个基因型，Cox B5为比例较高的基因型，占18.18%（44/242）。 2015 — 2018年，环境污水、健康儿童和AFP病例监测中的Cox B5分离株形成2个基因型和3个传播链，即C3、C4和D3基因亚型。 C3基因亚型在上海市属于优势基因亚型，而D3基因亚型是2016 — 2018年新出现的传播链。
    结论2015 — 2018年上海市2个区环境污水中常见的优势基因型为ECHO11、Cox B5、Cox B3和ECHO6。 Cox B5在环境污水、健康儿童和AFP病例中存在共循环现象。 我国尚未建立完善的NPEV相关疾病监测系统，环境污水监测可作为病例监测的重要补充方法。

     

    Abstract:
    ObjectiveTo understand the distribution of non-polio enterovirus (NPEV) isolated from environmental sewage in Minghang and Jiading district of Shanghai from 2015 to 2018, and explore the circulation and evolution of coxsackievirus B5 (Cox B5) in environmental sewage, healthy children and acute flaccid paralysis (AFP) cases.
    MethodsThe sewage plants in Minhang and Jiading were selected as long-term surveillance sites. Sewage samples were collected monthly from two sewage plants for concentration and enrichment. The supernatant was collected and inoculated with human rhabdomyosarcoma and Hep-2 cells for virus isolation. VP1 region was obtained by RT-PCR and sequenced.
    ResultsA total of 84 sewage samples were collected, in which 242 NPEV strains were isolated, and 19 genotypes were detected. Cox B5 was a genotype with high proportion, accounting for 18.18% (44/242). Cox B5 formed three transmission chains of two genotypes, namely C3, C4 and D3 genotypes. C3 was the predominant genotype in Shanghai, while the D3 genotype was a new transmission chain during 2016–2018.
    ConclusionFrom 2015 to 2018, the predominant genotypes of NPEV were ECHO11, Cox B5, Cox B3 and ECHO6 in environmental sewage in Shanghai. Cox B5 co-circulated in environmental sewage, healthy children and AFP cases. NPEV related disease surveillance system has not been established in China. Environmental sewage surveillance can be used as an important supplementary method for case surveillance.

     

    • HTML全文
    • 参考文献(22)
    • 相关文章
    • 施引文献
  • 资源附件(2)

/

返回文章
返回

在线交流