浙江省野生动物鼬獾和家犬狂犬病病毒L基因序列测定分析

王晓光 雷永良 柳付明 朱武洋 陶晓燕

王晓光, 雷永良, 柳付明, 朱武洋, 陶晓燕. 浙江省野生动物鼬獾和家犬狂犬病病毒L基因序列测定分析[J]. 疾病监测, 2020, 35(2): 104-109. doi: 10.3784/j.issn.1003-9961.2020.02.005
引用本文: 王晓光, 雷永良, 柳付明, 朱武洋, 陶晓燕. 浙江省野生动物鼬獾和家犬狂犬病病毒L基因序列测定分析[J]. 疾病监测, 2020, 35(2): 104-109. doi: 10.3784/j.issn.1003-9961.2020.02.005
Xiaoguang Wang, Yongliang Lei, Fuming Liu, Wuyang Zhu, Xiaoyan Tao. Sequence analysis on L gene of rabies virus isolated from wild Melogale moschata and domestic dogs in Zhejiang[J]. Disease Surveillance, 2020, 35(2): 104-109. doi: 10.3784/j.issn.1003-9961.2020.02.005
Citation: Xiaoguang Wang, Yongliang Lei, Fuming Liu, Wuyang Zhu, Xiaoyan Tao. Sequence analysis on L gene of rabies virus isolated from wild Melogale moschata and domestic dogs in Zhejiang[J]. Disease Surveillance, 2020, 35(2): 104-109. doi: 10.3784/j.issn.1003-9961.2020.02.005

浙江省野生动物鼬獾和家犬狂犬病病毒L基因序列测定分析

doi: 10.3784/j.issn.1003-9961.2020.02.005
基金项目: 国家科技重大专项(No.2018ZX10102001,No.2018ZX10713002)
详细信息
    作者简介:

    王晓光,男,内蒙古自治区赤峰市人,硕士,副主任技师,主要从事病原生物学、生物信息学研究工作,Email:xiaog_china@126.com

    通讯作者:

    陶晓燕,Tel:010–58900896,Email:txy212@126.com

  • 中图分类号: R512.99

Sequence analysis on L gene of rabies virus isolated from wild Melogale moschata and domestic dogs in Zhejiang

Funds: This study was supported by the fund for National Major Science and Technology Project (No.2018ZX10102001 and No.2018ZX10713002)
More Information
  • 摘要: 目的测定浙江省野生动物鼬獾和家犬狂犬病病毒L基因序列,在分子水平分析转录酶大蛋白(LP)的变异特点和遗传进化特征。方法对实验室检测阳性的狂犬病病毒标本进行L基因编码区核酸序列测定,通过生物分析软件比较L基因核苷酸序列和氨基酸序列,并与GenBank的36个参考序列编码区进行比对分析。结果测序获得野生动物鼬獾(F02、F04)和家犬(D01、D02)二种宿主来源的狂犬病病毒街毒株L基因核苷酸全长序列并递交GenBank,开放读码框(ORF)均为6 387 bp,编码区均以2个连续的起始密码子ATG起始,编码2 128个氨基酸。 在LP保守结构域上的特异功能短序列几乎完全保守,各结构域之间的序列保守性略差,对存在的变异位点绝大多数极性并没有变化,系统进化分析显示浙江省鼬獾、犬街毒株均归属于传统的狂犬病病毒 Asian谱系,与我国广泛传播的狂犬病病毒同属一种系。结论浙江省狂犬病病毒L基因的构成与传统狂犬病病毒一致,所构建进化树的分析作用与N基因相同,鼬獾病毒依然具有本土狂犬病病毒的基因特点,家犬病毒可能与我国多种宿主动物病毒来源相同或者具有新近的相互传播,不同宿主动物狂犬病病毒具有种群多样性的进化特点和地域性流行特征。
  • 图  1  L蛋白氨基酸序列熵分析

    注:熵(Hx)值从0(高度保守)到1.0(高可变区)不等,熵值小于0.1的氨基酸视为保守

    Figure  1.  Entropy (Hx) plot of L protein

    图  2  基于最大似然法的狂犬病病毒L基因进化树

    注:△. 浙江鼬獾分离株;◇. 浙江犬分离株;□. PV和CTN-1株;蓝色分支. China Ⅰ群;红色分支. China Ⅱ群;绿色分支. China Ⅲ群;紫色分支. China Ⅳ群;黄色分支. China Ⅴ群

    Figure  2.  Phylogenetic trees of sequences of L gene of rabies virus based on maximum likelihood

    表  1  浙江株与其他病毒株L蛋白保守结构域氨基酸序列相似性比较结果(%)

    Table  1.   Comparison of nucleotide acid and amino acid similarities of conserved domain of LP of rabies virus between Zhejiang strains and other strains(%)

     毒株宿主
    D01F02D01F02D01F02D01F02D01F02D01F02
    BD06犬   96.997.9100.099.098.296.4100.0100.0 99.297.0100.0 98.7
    BJ2011E马   97.498.4100.099.098.798.7100.0100.0100.097.9100.0 98.7
    CNIM1703羊   96.998.4100.099.096.997.8 99.4 99.4 97.597.9 98.7100.0
    CNM1101C牛   97.498.4100.099.098.798.7100.0100.0100.097.9100.0 98.7
    CTN-1人   95.896.9100.099.097.398.2100.0100.0 98.398.7 98.7100.0
    DRV鹿   95.396.4 98.197.196.997.8 98.8 98.8 97.597.9 97.4 98.7
    GD-SH-01猪   97.498.4100.099.097.399.1100.0100.0 98.399.6100.0 98.7
    JX08-45鼬獾  97.498.4100.099.096.999.6 99.4 99.4 97.599.6 98.7100.0
    MRV野鼠  95.396.4 98.197.197.898.7 99.4 99.4 96.696.6 96.1 97.4
    PV巴斯德株95.395.3100.099.094.695.5 98.8 98.8 95.896.2 98.7100.0
    WH11驴   97.498.4100.099.098.798.7100.0100.0 99.697.5100.0 98.7
    WQ14-RF狐狸  96.998.4100.099.097.398.2 99.4 99.4 97.097.5 98.7100.0
    均值96.697.6 99.798.797.498.1 99.6 99.6 98.197.9 98.9 99.1
    位置232~423504~607608~831889~1 0601 090~1 3261 673~1 747
    下载: 导出CSV
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  • 收稿日期:  2019-07-10
  • 网络出版日期:  2020-01-19
  • 刊出日期:  2020-02-01

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