康倩, 陈建华, 汪鹏, 张慧敏, 王雪莹, 张慧, 于德山. 2018年甘肃省兰州市诺如病毒G胃肠炎暴发疫情病原学分析[J]. 疾病监测, 2020, 35(3): 269-273. DOI: 10.3784/j.issn.1003-9961.2020.03.019
引用本文: 康倩, 陈建华, 汪鹏, 张慧敏, 王雪莹, 张慧, 于德山. 2018年甘肃省兰州市诺如病毒G胃肠炎暴发疫情病原学分析[J]. 疾病监测, 2020, 35(3): 269-273. DOI: 10.3784/j.issn.1003-9961.2020.03.019
Qian Kang, Jianhua Chen, Peng Wang, Huimin Zhang, Xueying Wang, Hui Zhang, Deshan Yu. Molecular etiologic investigation of gastroenteritis outbreaks caused by Norovirus G in two schools in Lanzhou, 2018[J]. Disease Surveillance, 2020, 35(3): 269-273. DOI: 10.3784/j.issn.1003-9961.2020.03.019
Citation: Qian Kang, Jianhua Chen, Peng Wang, Huimin Zhang, Xueying Wang, Hui Zhang, Deshan Yu. Molecular etiologic investigation of gastroenteritis outbreaks caused by Norovirus G in two schools in Lanzhou, 2018[J]. Disease Surveillance, 2020, 35(3): 269-273. DOI: 10.3784/j.issn.1003-9961.2020.03.019

2018年甘肃省兰州市诺如病毒G胃肠炎暴发疫情病原学分析

Molecular etiologic investigation of gastroenteritis outbreaks caused by Norovirus G in two schools in Lanzhou, 2018

  • 摘要:
    目的了解2018年5—6月甘肃省兰州市发生的2起学校急性胃肠炎暴发疫情的诺如病毒分子特征,并进行溯源分析。
    方法采用实时荧光定量反转录聚合酶链式反应对标本进行诺如病毒检测,对阳性标本聚合酶区–衣壳蛋白区核苷酸进行测序和同源性分析,采用 MEGA 6.0 软件进行遗传进化分析。
    结果2起学校胃肠炎疫情均为诺如病毒GⅠ引起,5月28日至6月4日的一起疫情发病50例,罹患率为3.24%。 6月1—13日的疫情发病35例,罹患率为4.76%;流行病学调查提示,2起疫情均与小饭桌就餐和密切接触相关。 33份标本中检出诺如病毒GⅠ核酸阳性标本13份。 对阳性样本的RdRP和VP1区的部分基因片段进行测序和比对,第1起疫情为诺如病毒 GⅠ.3[P13] 基因型,第2起疫情为GⅠ.6[P11]基因型。
    结论兰州市城关区2起学校急性胃肠炎疫情分别由 GⅠ.3[P13]型和GⅠ.6[P11]型诺如病毒引起,应加强诺如病毒监测,强化中小学附近小饭桌等集中就餐场所的管理。

     

    Abstract:
    ObjectiveTo understand the molecular characteristics of Norovirus-caused outbreaks of acute gastroenteritis in two schools in Lanzhou from May to June in 2018, and analyze the sources of Norovirus infections.
    MethodsTwenty nine anal swabs and 4 surface swabs from the two outbreaks were detected for Norovirus by real-time fluorescence quantitative RT-PCR. The RdRp and VP1 region genes of positive samples were sequenced and analyzed by NCBI and MEGA 6.0 software.
    ResultsThe outbreaks of gastroenteritis in two schools were caused by Norovirus GⅠ. In the outbreak in school A from 28 May to 4 June, 50 cases occurred with an attack rate of 3.24%. In the outbreak in school B from 1 to 13 June, 35 cases occurred with an attack arte of 4.76%. The epidemiological investigation showed that the two outbreaks were related to small dining place and close contact. Thirteen out of 33 samples were positive for Norovirus GⅠ nucleic acid. The gene sequencing and alignment results indicated that the first epidemic was caused by Norovirus GⅠ.3 P13, and the second one was caused by Norovirus GⅠ.6 P11.
    Conclusion Two outbreaks of acute gastroenteritis in two schools in Chengguan district of Lanzhou were caused by Norovirus GⅠ.3 P13 and GⅠ.6 P11 respectively. It is necessary to strengthen the surveillance for Norovirus and the management of small dining places near middle and primary schools.

     

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