新疆维吾尔自治区人间布鲁氏菌多位点可变数目串联重复序列分型研究

李博; 姜海; 崔步云; 黎唯; 刘志国; 尚修建

doi:10.3784/j.issn.1003-9961.2020.05.011

新疆维吾尔自治区人间布鲁氏菌多位点可变数目串联重复序列分型研究

Multilocus variable-number tandem repeat analysis on clinical strains of Brucella isolated in Xinjiang

摘要

摘要:
目的采用多位点可变数目串联重复序列分析（MLVA）方法对新疆维吾尔自治区（新疆）分离的人间布鲁氏菌进行基因型分析。
方法采用布鲁氏菌MLVA16-多色毛细管电泳分型方法，对2015年和2016年分离自新疆7个地（州、市）的24株人间布鲁氏菌临床分离株和3株布鲁氏菌标准菌株进行分型；利用BioNumerics（Version 7.5）软件构建菌株最小进化树，分析菌株间遗传关系。
结果MLVA16分型可将不同种布鲁氏菌予以区分，24株临床分离株panel1、panel2A均为42型和108型，panel2B具有多态性；24株临床分离株被聚类为一个分支，与羊种布鲁氏菌聚类为一个大分支，个别不同地区分离株的亲缘关系较近。
结论羊种3型布鲁氏菌是新疆人间布鲁氏菌病（布病）的主要流行菌株，MLVA16-多色毛细管电泳分型作为一种快速、可靠的基因分型方法，可为新疆人间布病传染源的溯源研究提供依据。

Abstract:
ObjectiveMultilocus variable-number tandem repeat analysis (MLVA) was conducted for the genotyping of the clinical Brucella strains isolated in Xinjiang to understand the population structure and genetic evolution of the isolated strains.
MethodsA total 24 Brucella clinical strains isolated from 7 prefectures in Xiniang in 2015 and 2016 and 3 standard reference strains were analyzed by MLVA-16 using multicolor capillary electrophoresis to determine sequences types. In order to analyze the genetic relationship among isolated strains, we constructed a minimum spanning tree by using BioNumerics.
ResultsMLVA-16 could distinguish different species of Brucella, the genotype of panel1 and panel2A of 24 strains were all 42 and 108, while locus of panel2B showed polymorphism. The results of the cluster analysis showed that 24 strains were in a branch and in a large branch with Brucella melitensis. Some isolates had close genetic distance with isolates in other regions.
ConclusionThe Brucella melitensis serotype 3 was the major pathogen causing brucellosis in Xinjiang. MLVA-16 using multicolor capillary electrophoresis is a rapid and reliable genotyping method, which can provide evidence for traceability of human brucellosis in Xinjiang.

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