王铜, 陶晓霞, 郭敏卓, 孟凡亮, 王多, 张建中, 王国庆, 闫笑梅. 金黄色葡萄球菌肠毒素SEA、SEB、SEC、SED序列多态性及进化分析[J]. 疾病监测, 2020, 35(10): 926-933. DOI: 10.3784/j.issn.1003-9961.2020.10.013
引用本文: 王铜, 陶晓霞, 郭敏卓, 孟凡亮, 王多, 张建中, 王国庆, 闫笑梅. 金黄色葡萄球菌肠毒素SEA、SEB、SEC、SED序列多态性及进化分析[J]. 疾病监测, 2020, 35(10): 926-933. DOI: 10.3784/j.issn.1003-9961.2020.10.013
Tong Wang, Xiaoxia Tao, Minzhuo Guo, Fanliang Meng, Duo Wang, Jianzhong Zhang, Guoqing Wang, Xiaomei Yan. Sequence polymorphisms and evolutionary analysis of Staphylococcus aureus enterotoxin A, B, C and D[J]. Disease Surveillance, 2020, 35(10): 926-933. DOI: 10.3784/j.issn.1003-9961.2020.10.013
Citation: Tong Wang, Xiaoxia Tao, Minzhuo Guo, Fanliang Meng, Duo Wang, Jianzhong Zhang, Guoqing Wang, Xiaomei Yan. Sequence polymorphisms and evolutionary analysis of Staphylococcus aureus enterotoxin A, B, C and D[J]. Disease Surveillance, 2020, 35(10): 926-933. DOI: 10.3784/j.issn.1003-9961.2020.10.013

金黄色葡萄球菌肠毒素SEA、SEB、SEC、SED序列多态性及进化分析

Sequence polymorphisms and evolutionary analysis of Staphylococcus aureus enterotoxin A, B, C and D

  • 摘要:
    目的对不同来源金黄色葡萄球菌肠毒素SEA、SEB、SEC和SED的序列多态性及进化进行分析,为进一步了解不同亚型的毒素表达差异及食品风险评估提供理论依据。
    方法选择我国部分地区不同来源经典肠毒素SEA、SEB、SEC和SED阳性的菌株共90株,采用PCR方法扩增其序列并测序。 并与GenBank下载的经典肠毒素亚型代表序列进行比对分析,用MEGA 7软件构建系统发生树。
    结果SEA氨基酸序列存在3个突变位点,主要位于N端和C端;有3个亚型,SEA阳性菌株以SEA1亚型为主。 SEB氨基酸序列在两端的突变较中间活跃,存在18个突变位点;有11个亚型,SEB阳性菌株主要为SEB1和SEB2亚型。 SEC氨基酸序列突变位点有24个,分布在整个序列;共8个亚型,SEC阳性菌株以SEC3亚型为主。 SED氨基酸序列相对保守,共7个亚型,SED阳性菌株以SED1为主。 本研究还发现2个新亚型,分别命名为SEB9和SEC5,均来自患者分离菌株。
    结论SEA和SED的氨基酸序列较为保守,SEB和SEC的氨基酸突变位点较多。4种肠毒素的氨基酸序列进化树均分为2个分支。 本研究选取的菌株以SEA1、SEB1、SEB2、SEC3和SED1亚型为主。

     

    Abstract:
    ObjectiveTo analyze the sequence polymorphism and evolution of Staphylococcal aureus enterotoxin (SE) A, B, C and D from different sources and provide theoretical basis for further understanding the variation in SE expression among different subtypes and food safety risk assessment.
    MethodsA total of 90 strains which were positive for enterotoxin A, B, C or D from different sources were selected and sequenced, and the results were compared with the sequences of the representative subtypes of enterotoxins downloaded from GenBank, and the phylogenetic tree was constructed by MEGA 7.
    ResultsThere were three mutation sites in the amino acid sequences of SEA, which were mainly located on both sides of the N-terminal and C-terminal, and there were three subtypes. The main subtype of the strains in this study was SEA1. The mutation sites of the amino acid sequences of SEB were mainly located in the middle of the sequence. There were 18 mutation sites and 11 subtypes. The main subtypes of the strains in this study were SEB1 and SEB2. There were 24 amino acid mutation sites in SEC, which were distributed in the whole sequence and divided into 8 subtypes. SEC3 was the main subtype in this study. The amino acid sequences of SED were relatively conserved, including 7 subtypes. The main subtype in this study was SED1. At the same time, two new subtypes, SEB9 and SEC5, were also found in this study.
    ConclusionThe amino acid sequences of SEA and SED were relatively conservative compared with SEB and SEC. The phylogenetic trees of SEA, SEB, SEC and SED were all divided into two branches. The main subtypes of strains selected in this study were SEA1, SEB1, SEB2, SEC3 and SED1.

     

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