2012-2018年江苏省无锡市肠炎沙门菌药物敏感性与分子分型研究

齐倩倩 沙丹 李娟 赵晓菲 郑东宇 管红霞

齐倩倩, 沙丹, 李娟, 赵晓菲, 郑东宇, 管红霞. 2012-2018年江苏省无锡市肠炎沙门菌药物敏感性与分子分型研究[J]. 疾病监测, 2020, 35(12): 1146-1151. doi: 10.3784/j.issn.1003-9961.2020.12.018
引用本文: 齐倩倩, 沙丹, 李娟, 赵晓菲, 郑东宇, 管红霞. 2012-2018年江苏省无锡市肠炎沙门菌药物敏感性与分子分型研究[J]. 疾病监测, 2020, 35(12): 1146-1151. doi: 10.3784/j.issn.1003-9961.2020.12.018
Qi Qianqian, Sha Dan, Li Juan, Zhao Xiaofei, Zheng Dongyu, Guan Hongxia. Drug resistance and molecular types of Salmonella Enteritidis in Wuxi, Jiangsu, 2012–2018[J]. Disease Surveillance, 2020, 35(12): 1146-1151. doi: 10.3784/j.issn.1003-9961.2020.12.018
Citation: Qi Qianqian, Sha Dan, Li Juan, Zhao Xiaofei, Zheng Dongyu, Guan Hongxia. Drug resistance and molecular types of Salmonella Enteritidis in Wuxi, Jiangsu, 2012–2018[J]. Disease Surveillance, 2020, 35(12): 1146-1151. doi: 10.3784/j.issn.1003-9961.2020.12.018

2012-2018年江苏省无锡市肠炎沙门菌药物敏感性与分子分型研究

doi: 10.3784/j.issn.1003-9961.2020.12.018
基金项目: 无锡市卫生健康委项目[No. CXPT(SYS)002]
详细信息
    作者简介:

    齐倩倩,女,河南省鹤壁市人,硕士,检验技师,主要从事肠道病原菌检测工作,Email:18360865672@163.com

    通讯作者:

    管红霞,Tel:13812075983,Email:ghx-331@163.com

  • 中图分类号: R211; R378.2

Drug resistance and molecular types of Salmonella Enteritidis in Wuxi, Jiangsu, 2012–2018

Funds: This study was supported by the fund for Wuxi Municipal Health Commission Project [No. CXPT (SYS) 002]
More Information
  • 摘要:   目的   分析2012 — 2018年江苏省无锡市肠炎沙门菌分离株的药物敏感性和分子分型。   方法   收集2012 — 2018年分离自无锡市食源性疾病监测哨点医院门诊腹泻患者、从业体检人员、食物中毒以及环境水样监测的110株肠炎沙门菌菌株,采用BD Phoenix全自动细菌鉴定/药敏系统测试方案和脉冲场凝胶电泳(PFGE)方法,进行药物敏感性试验和PFGE分子分型分析。   结果   110株肠炎沙门菌对环丙沙星、厄他培南、亚胺培南、美罗培南、阿奇霉素和头孢他啶/阿维巴坦6种抗生素均敏感,对萘啶酮酸、氨苄西林和氨苄西林/舒巴坦的耐药率较高;77(70.00%)株菌为多重耐药,以耐3种抗生素为主(45.45%,50/110)。PFGE将110株菌分为39个带型,各带型包含1~31株菌,EN17、EN21和EN24为优势带型。   结论   无锡市肠炎沙门菌耐药状况严重,应引起重视,菌株的分子分型多样,具有优势带型。
  • 图  1  2015-2018年分离的肠炎沙门菌的耐药情况

    Figure  1.  Antibiotic resistance of Salmonella Enteritidis isolated from 2015 to 2018

    图  2  110株肠炎沙门菌的PFGE聚类图

    Figure  2.  PFGE patterns of 110 Salmonella Enteritidis strains

    表  1  健康人和腹泻患者来源肠炎沙门菌的耐药性比较

    Table  1.   Antibiotic resistance of Salmonella Enteritidis isolated from healthy people and diarrhea patients

    抗生素健康人来源腹泻患者来源χ2P
    耐药
    菌株数
    (株)
    耐药率
    (%)
    耐药
    菌株数
    (株)
    耐药率
    (%)
    四环素 425.001719.770.2260.635
    呋喃妥因 1 6.25 3 3.490.2730.601
    头孢噻肟 1 6.25 910.470.2710.603
    头孢他啶 1 6.25 910.470.2710.603
    萘啶酮酸1487.507991.860.3190.572
    复方磺胺甲恶唑 1 6.25 910.470.2710.603
    氨曲南 1 6.25 8 9.300.1560.693
    氨苄西林1593.756879.071.9180.166
    氨苄西林/舒巴坦1168.754653.491.2740.259
    氯霉素 1 6.25 7 8.140.0670.796
    下载: 导出CSV

    表  2  110株肠炎沙门菌的耐药谱

    Table  2.   Antibiotic resistance spectrum of 110 Salmonella Enteritidis strains

    耐抗生素
    种类(种)
    耐药谱菌株数(株)构成比(%)
    1 NAL 20 18.18
    2 AMP-NAL 7 6.36
    AMP-TET 2 1.82
    NAL-F 1 0.91
    3 AMP-SAM-NAL 41 37.27
    AMP-NAL-SXT 3 2.73
    AMP-SAM-TET 2 1.82
    AMP-NAL-TET 2 1.82
    AMP-CHL-NAL 1 0.91
    AMP-TET-SXT 1 0.91
    4 AMP-SAM-NAL-TET 8 7.27
    AMP-CHL-TET-SXT 2 1.82
    AMP-NAL-TET-SXT 2 1.82
    AMP-CHL-TET-SXT 1 0.91
    AMP-SAM-CHL-NAL 1 0.91
    AMP-SAM-NAL-F 1 0.91
    5 AMP-ATM-CTX-CAZ-NAL 6 5.45
    AMP-SAM-CHL-NAL-TET 1 0.91
    AMP-SAM-CTX-CAZ-NAL 1 0.91
    6 AMP-SAM-ATM-CTX-CAZ-NAL 2 1.82
    7 AMP-SAM-CHL-NAL-F-TET-SXT 1 0.91
    8 AMP-ATM-CTX-CAZ-CHL-NAL-F-TET 1 0.91
      注:TET. 四环素;F. 呋喃妥因;CTX. 头孢噻肟;CAZ. 头孢他啶;NAL. 萘啶酮酸;SXT. 复方磺胺甲恶唑;ATM. 氨曲南;AMP. 氨苄西林;SAM. 氨苄西林/舒巴坦;CHL. 氯霉素
    下载: 导出CSV
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  • 收稿日期:  2020-08-21
  • 网络出版日期:  2020-11-30
  • 刊出日期:  2020-12-30

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