2013-2019年浙江省湖州市急性胃肠炎病例G.P7-G.6型诺如病毒基因特征分析

纪蕾 刘光涛 沈月华 查赟峰 徐德顺

纪蕾, 刘光涛, 沈月华, 查赟峰, 徐德顺. 2013-2019年浙江省湖州市急性胃肠炎病例GⅡ.P7-GⅡ.6型诺如病毒基因特征分析[J]. 疾病监测, 2021, 36(1): 53-58. doi: 10.3784/jbjc.202006180218
引用本文: 纪蕾, 刘光涛, 沈月华, 查赟峰, 徐德顺. 2013-2019年浙江省湖州市急性胃肠炎病例G.P7-G.6型诺如病毒基因特征分析[J]. 疾病监测, 2021, 36(1): 53-58. doi: 10.3784/jbjc.202006180218
Ji Lei, Liu Guangtao, Shen Yuehua, Zha Yunfeng, Xu Deshun. Genetic characteristics of norovirus GⅡ.P7-GⅡ.6 isolated from acute gastroenteritis cases in Huzhou of Zhejiang, 2013–2019[J]. Disease Surveillance, 2021, 36(1): 53-58. doi: 10.3784/jbjc.202006180218
Citation: Ji Lei, Liu Guangtao, Shen Yuehua, Zha Yunfeng, Xu Deshun. Genetic characteristics of norovirus G.P7-G.6 isolated from acute gastroenteritis cases in Huzhou of Zhejiang, 2013–2019[J]. Disease Surveillance, 2021, 36(1): 53-58. doi: 10.3784/jbjc.202006180218

2013-2019年浙江省湖州市急性胃肠炎病例G.P7-G.6型诺如病毒基因特征分析

doi: 10.3784/jbjc.202006180218
基金项目: 浙江省医药卫生科技计划(No. 2018RC015)
详细信息
    作者简介:

    纪蕾,女,安徽省淮南市人,主管技师,从事微生物检验研究

    通讯作者:

    纪蕾,Tel:0572–2760126,Email:jileichn@163.com

  • 中图分类号: R211;R373.2

Genetic characteristics of norovirus G.P7-G.6 isolated from acute gastroenteritis cases in Huzhou of Zhejiang, 2013–2019

Funds: This study was supported by the fund for Zhejiang Medical Science and Technology Program (No. 2018RC015)
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  • 摘要:   目的  分析2013 — 2019年浙江省湖州市急性胃肠炎病例诺如病毒基因特征,为疾病监测和防控提供参考。  方法  采用荧光定量反转录聚合酶链式反应(RT-PCR)方法对2013年12月、2014年4、12月、2019年3月发生的4起学校/托幼机构急性胃肠炎患者送检粪便标本进行诺如病毒核酸检测。 采用RT-PCR法对核酸阳性标本的多聚酶和衣壳蛋白部分区域进行扩增,并对扩增产物进行序列测定。 利用在线分型工具和系统进化分析对病原序列进行基因特征分析。  结果  共计28份标本中分别有5、4、2、5份为GⅡ型诺如病毒核酸阳性,阳性率为57.1%(16/28)。 4次疫情各有1份标本测序成功;在线分型和系统进化分支分析显示,4起疫情的病原均为GⅡ.P7-GⅡ.6重组型诺如病毒,但进化分支不同,其中2013年疫情标本检出GⅡ.P7-GⅡ.6c型;2014年2起疫情均为GⅡ.P7-GⅡ.6b型,2019年疫情中检出的GⅡ.P7-GⅡ.6a型。  结论  GⅡ.P7-GⅡ.6型重组型诺如病毒是引起湖州市2013 — 2019年4起急性胃肠炎暴发疫情的病原体,每年毒株存在一定基因进化分支的差异。 鉴于该病毒在全球范围内具有持续和广泛的流行能力,应进一步加强对该型别重组型诺如病毒的监测。
  • 图  1  诺如病毒衣壳蛋白片段的系统进化分析

              注:●. 本研究检出的诺如病毒

    Figure  1.  Phylogenetic analysis based on partial capsid gene sequences of norovirus

    图  2  诺如病毒部分多聚酶区片段的系统进化分析

               注:●. 本研究检出的诺如病毒

    Figure  2.  Phylogenetic analysis based on partial RdRp gene sequences of norovirus

    图  3  诺如病毒RdRp区至衣壳蛋白区的长片段系统进化分析

    注:●. 本研究检出的NoV;进化树中红色字体分别是GⅡ.P7-GⅡ.6a代表序列IT/2011/150/16-PA309、GⅡ.P7-GⅡ.6b代表序列US/2012/BethesdaD1和GⅡ.P7-GⅡ.6c代表序列US/2010/HS245

    Figure  3.  Phylogenetic analysis based on partial RdRp and capsid gene sequences of norovirus

    表  1  湖州市急性胃肠炎病例诺如病毒感染概况

    Table  1.   Infection status of norovirus in acute gastroenteritis cases in Huzhou

    场所日期
    (年–月)
    病例数(例)平均年龄(岁)不同症状病例数(例)和百分比(%)采样检测数(份)检测阳性数(份)和阳性率(%)测序标本
    发热呕吐腹泻
    小学12013–1226 72(7.7)26(100.0) 6(23.0) 9 5(55.6)N13133
    幼儿园12014–0414 40(0.0)13(92.9) 4(28.6) 6 4(66.7)N14024
    幼儿园22014–1111 40(0.0)11(100.0) 3(27.3) 5 2(40.0)N14042
    小学22019–0324101(4.2)22(91.7) 4(16.7) 8 5(62.5)N19001
    合计753(4.0)72(96.0) 17(22.7)2816(57.1)
    下载: 导出CSV
  • [1] Kapikian AZ, Wyatt RG, Dolin R, et al. Visualization by immune electron microscopy of a 272-nm particle associated with acute infectious nonbacterial gastroenteritis[J]. J Virol, 1972, 10(5): 1075–1081. DOI: 10.1128/JVI.10.5.1075−1081.1972.
    [2] Patel MM, Hall AJ, Vinjé J, et al. Noroviruses: a comprehensive review[J]. J Clin Virol, 2009, 44(1): 1–8. DOI:  10.1016/j.jcv.2008.10.009.
    [3] 廖巧红, 冉陆, 靳淼, 等. 诺如病毒感染暴发调查和预防控制技术指南(2015版)[J]. 中华预防医学杂志,2016,6(1):7–16. DOI:10.3760/cma.j.issn.0253−9624.2016.01.003.

    Liao QH, Ran L, Jin M, et al. Guidelines on outbreak investigation, prevention and control of norovirus infection (2015)[J]. Chin J Prev Med, 2016, 6(1): 7–16. DOI: 10.3760/cma.j.issn.0253−9624.2016.01.003.
    [4] Vinje J. Advances in laboratory methods for detection and typing of norovirus[J]. J Clin Microbiol, 2015, 53(2): 373–381. DOI: 10.1128/JCM.01535−14.
    [5] Chhabra P, De Graaf M, Parra GI, et al. Updated classification of norovirus genogroups and genotypes[J]. J Gen Virol, 2019, 100(10): 1393–1406. DOI:  10.1099/jgv.0.001318.
    [6] Bull RA, Tanaka MM, White PA. Norovirus recombination[J]. J Gen Virol, 2007, 88(12): 3347–3359. DOI: 10.1099/vir.0.83321−0.
    [7] Beuret C, Kohler D, Baumgartner A, et al. Norwalk-like virus sequences in mineral waters: one-year monitoring of three brands[J]. Appl Environ Microbiol, 2002, 68(4): 1925–1931. DOI: 10.1128/aem.68.4.1925−1931.
    [8] Vennema H, De Bruin E, Koopmans M. Rational optimization of generic primers used for Norwalk-like virus detection by reverse transcriptase polymerase chain reaction[J]. J Clin Virol, 2002, 25(2): 233–235. DOI: 10.1016/s1386−6532(02)00126−9.
    [9] Ao YY, Wang JJ, Ling H, et al. Norovirus GⅡ.P16/GⅡ.2-associated gastroenteritis, China, 2016[J]. Emerg Infect Dis, 2017, 23(7): 1172–1175. DOI:  10.3201/eid2307.170034.
    [10] Bidalot M, Théry L, Kaplon J, et al. Emergence of new recombinant noroviruses GⅡ.p16-GⅡ.4 and GⅡ.p16-GⅡ.2, France, winter 2016 to 2017[J]. Euro Surveill, 2017, 22(15): 30508. DOI: 10.2807/1560−7917.ES.2017.22.15.30508.
    [11] Bruggink LD, Moselen JM, Marshall JA. The comparative molecular epidemiology GⅡ.7_GⅡ.6 and GⅡ.P7_GⅡ.7 norovirus outbreaks in Victoria, Australia, 2012–2014[J]. Intervirology, 2016, 59(1): 60–65. DOI:  10.1159/000448100.
    [12] Fumian TM, Da Silva Ribeiro de Andrade J, Leite JPG, et al. Norovirus recombinant strains isolated from gastroenteritis outbreaks in Southern Brazil, 2004–2011[J]. PLoS One, 2016, 11(4): e0145391. DOI:  10.1371/journal.pone.0145391.
    [13] Diakoudi G, Lanave G, Catella C, et al. Analysis of GⅡ.P7 and GⅡ.6 noroviruses circulating in Italy during 2011–2016 reveals a replacement of lineages and complex recombination history[J]. Infect Genet Evol, 2019, 75: 103991. DOI:  10.1016/j.meegid.2019.103991.
    [14] Yang ZH, Vinjé J, Elkins CA, et al. Complete genome sequence of human norovirus strain GⅡ.P7-GⅡ.6 detected in a patient in the United States in 2014[J]. Genome Announc, 2016, 4(5): e01211–16. DOI: 10.1128/genomeA.01211−16.
    [15] Sakon N, Komano J, Tessmer HL, et al. High transmissibility of norovirus among infants and school children during the 2016/17 season in Osaka, Japan[J]. Euro Surveill, 2018, 23(6): 18–00029. DOI: 10.2807/1560−7917.
    [16] Cai H, Yu Y, Jin M, et al. Cloning, sequencing and characterization of the genome of a recombinant norovirus of the rare genotype GⅡ.P7/GⅡ.6 in China[J]. Arch Virol, 2017, 162(7): 2053–2059. DOI: 10.1007/s00705−017−3325−1.
    [17] Qin SW, Chan TC, Cai J, et al. Genotypic and epidemiological trends of acute gastroenteritis associated with noroviruses in China from 2006 to 2016[J]. Int J Environ Res Public Health, 2017, 14(11): 1341. DOI:  10.3390/ijerph14111341.
    [18] Ji L, Wu XF, Yao WT, et al. Rapid emergence of novel GⅡ.4 sub-lineages noroviruses associated with outbreaks in Huzhou, China, 2008–2012[J]. PLoS One, 2013, 8(12): e82627. DOI:  10.1371/journal.pone.0082627.
    [19] Wu XF, Han JK, Chen LP, et al. Prevalence and genetic diversity of noroviruses in adults with acute gastroenteritis in Huzhou, China, 2013–2014[J]. Arch Virol, 2015, 160(7): 1705–1713. DOI: 10.1007/s00705−015−2440−0.
    [20] Han JK, Wu XF, Chen LP, et al. Emergence of norovirus GⅡ.P16-GⅡ.2 strains in patients with acute gastroenteritis in Huzhou, China, 2016–2017[J]. BMC Infect Dis, 2018, 18(1): 342. DOI: 10.1186/s12879−018−3259−6.
    [21] Cannon JL, Barclay L, Collins NR, et al. Genetic and epidemiologic trends of norovirus outbreaks in the United States from 2013 to 2016 demonstrated emergence of novel GⅡ 4. recombinant viruses[J]. J Clin Microbiol, 2017, 55(7): 2208–2221. DOI: 10.1128/JCM.00455−17.
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  • 收稿日期:  2020-06-18
  • 网络出版日期:  2020-10-19
  • 刊出日期:  2021-02-08

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