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孙灵利, 韩桃利, 齐啸, 黄洋, 张越, 高艳, 赵剑虹, 李蓓蓓, 张政, 徐潇, 焦洋. 2018－2023年北京市朝阳区急性胃肠炎聚集性疫情中肠道腺病毒和星状病毒的病原特征研究[J]. 疾病监测. DOI: 10.3784/jbjc.2021.000
引用本文: 孙灵利, 韩桃利, 齐啸, 黄洋, 张越, 高艳, 赵剑虹, 李蓓蓓, 张政, 徐潇, 焦洋. 2018－2023年北京市朝阳区急性胃肠炎聚集性疫情中肠道腺病毒和星状病毒的病原特征研究[J]. 疾病监测. DOI: 10.3784/jbjc.2021.000
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Sun Lingli, Han Taoli, Qi Xiao, Huang Yang, Zhang Yue, Gao Yan, Zhao Jianhong, Li Beibei, Zhang Zheng, Xu Xiao, Jiao Yang. Pathogenic Characteristics of Acute Gastroenteritis Outbreaks Caused by Astrovirus and Enteric Adenovirus in Beijing Chaoyang from 2018 to 2023[J]. Disease Surveillance. DOI: 10.3784/jbjc.2021.000
Citation: Sun Lingli, Han Taoli, Qi Xiao, Huang Yang, Zhang Yue, Gao Yan, Zhao Jianhong, Li Beibei, Zhang Zheng, Xu Xiao, Jiao Yang. Pathogenic Characteristics of Acute Gastroenteritis Outbreaks Caused by Astrovirus and Enteric Adenovirus in Beijing Chaoyang from 2018 to 2023[J]. Disease Surveillance. DOI: 10.3784/jbjc.2021.000
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2018－2023年北京市朝阳区急性胃肠炎聚集性疫情中肠道腺病毒和星状病毒的病原特征研究

Pathogenic Characteristics of Acute Gastroenteritis Outbreaks Caused by Astrovirus and Enteric Adenovirus in Beijing Chaoyang from 2018 to 2023

    摘要
  • 摘要:
    目的  探讨2018—2023年期间北京市朝阳区急性胃肠炎（acute gastroenteritis，AGE）聚集性疫情中肠道腺病毒（Adenovirus，AdV）和星状病毒（Astrovirus，AstV）的病原特征。
    方法  采用荧光定量PCR 法对2018年1月至2023年12月期间北京市朝阳区报告的AGE聚集性疫情的粪便/肛拭子样本进行肠道AdV和AstV核酸检测。采用反转录PCR或PCR方法对核酸阳性样本进行目的基因扩增测序、基因分型和进化分析，获得AGE聚集性疫情中肠道AdV和AstV的感染情况及基因型别，采用Mega6.0软件进行系统进化分析。
    结果  2018—2023年朝阳区AGE聚集性疫情中，分别在18起（2.4%）和23起（3.1%）疫情样本中检出肠道AdV和AstV核酸，两者均主要发生在幼儿园。在肠道AdV和AstV感染病例中，男女比例分别为1∶1和1.7∶1，年龄中位数分别为4和5岁。在成功完成序列测定的11起肠道AdV阳性和16起AstV样本中，系统进化分析显示肠道AdV均为AdV41型，而AstV有4 种基因型，包括AstV-1（75.0%）、AstV-2（6.3%）、AstV-3（6.3%）和AstV-5（12.5%）。
    结论  本研究对2018—2023年期间，北京市朝阳区急性胃肠炎聚集性疫情中肠道腺病毒和星状病毒的病原特征进行了系统的分析，肠道AdV41型和AstV-1型为主要流行基因型，需要加强对AGE疫情中的病原特征的持续监测。

     

    Abstract:
    Objective  To investigate the pathogenic characteristics of Enteric Adenovirus (AdV) and Astrovirus (AstV) in acute gastroenteritis (AGE) outbreaks in Beijing Chaoyang District from 2018 to 2023.
    Methods  Fecal/rectal swab samples from AGE outbreaks in Chaoyang District from January 2018 to December 2023 were collected and the Enteric AdV and AstV nucleic acids were detected by Real-time PCR. Then RT-PCR or PCR was performed on the positive samples for target gene amplification and sequencing, followed by genotyping and evolutionary analysis of Enteric AdV and AstV in the AGE outbreaks. The infection status and genotypes of Enteric AdV and AstV were obtained, and the evolutionary analysis was conducted using Mega6.0 software.
    Results  Enteric AdV and AstV were detected in 18 and 23 outbreaks, accounting for 2.4% and 3.1% of the AGE outbreaks respectively. The outbreaks mainly occurred in kindergartens. The male-to-female ratio of cases was 1∶1 for Enteric AdV and 1.7∶1 for AstV, with a median age of 4 (Enteric AdV) and 5 (AstV) years. Sequencing was successfully completed for 11 (Enteric AdV) and 16 (AstV) positive outbreaks. The evolutionary analysis revealed that all of the Enteric AdV belonged to AdV41 type and AstV constituted by HAstV-1 (75.0%), HAstV-2 (6.25%), HAstV-3 (6.25%), and HAstV-5 (12.5%).
    Conclusion  This study provided a systematic analysis of the pathogenic characteristics of Enteric AdV and AstV in AGE outbreaks in Beijing Chaoyang District from 2018 to 2023. The predominant circulating genotypes were AdV41 and HAstV-1, highlighting the need for continuous monitoring of the pathogenic characteristics in acute gastroenteritis outbreaks.

     

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