肠球菌菌种鉴定方法的探索

白一波; 徐明超; 焦一帆; 周娟; 张思慧; 杨晶; 刘丽云; 徐建国

doi:10.3784/jbjc.202102210083

肠球菌菌种鉴定方法的探索

Exploration of methods for identification of Enterococcus

摘要

摘要:
目的选择一种能将肠球菌属鉴定到种水平的快速准确的方法。
方法分别使用生化鉴定方法、16S rRNA基因测序技术、rpoA基因测序技术、基因组杂交技术（DNA–DNA hybridization，DDH）和平均核苷酸相似度（Average nucleotide identity，ANI）对12株肠球菌进行种水平鉴定，并比较生化鉴定方法、16S rRNA基因测序技术和rpoA基因测序技术和DDH/ANI结果的一致性。
结果经过DDH和ANI鉴定，分离自高原野生岩羊粪便的12株肠球菌中有5株屎肠球菌，4株海氏肠球菌，1株蒙氏肠球菌，1株鹑鸡肠球菌和1株疑似肠球菌属内新种，rpoA基因序列比对结果与DDH/ANI结果高度一致，生化鉴定和16S rRNA基因测序技术与DDH/ANI结果不完全相符。
结论 rpoA基因序列比对可以方便快捷准确的将肠球菌鉴定到种水平。

Abstract:
Objective To select a rapid and accurate method to identify Enterococcus at genus level.
Methods By using biochemical identification, 16S rRNA gene sequencing, rpoA gene sequencing, DNA-DNA hybridization (DDH) and average nucleotide identity (ANI), 12 Enterococcus strains were identified at genus level., and the results were compared.
Results By DDH and ANI identifications, 12 Enterococcus strains isolated from stool samples of wild blue sheep in plateau area were classified as Enterococcus faecium (5 strains), Enterococcus heisei (4 strains), Enterococcus montmund (1 strain), Enterococcus quail (1 strain) and suspected new Enterococcus variant (1 strain). rpoA gene sequencing had highly consistent results with DDH/ANI, but biochemical identification, 16S rRNA gene sequencing had no fully consistent results with DDH/ANI.
Conclusion rpoA gene sequencing can be used to identify Enterococcus rapidly and accurately.

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