类鼻疽伯克霍尔德菌在青瓜和生菜幼苗中的定植情况研究

陈少金 王立程 朱雄 符晓莹 陈海 梁丽娟 韩丽梅

陈少金, 王立程, 朱雄, 符晓莹, 陈海, 梁丽娟, 韩丽梅. 类鼻疽伯克霍尔德菌在青瓜和生菜幼苗中的定植情况研究[J]. 疾病监测, 2021, 36(9): 938-942. doi: 10.3784/jbjc.202103160128
引用本文: 陈少金, 王立程, 朱雄, 符晓莹, 陈海, 梁丽娟, 韩丽梅. 类鼻疽伯克霍尔德菌在青瓜和生菜幼苗中的定植情况研究[J]. 疾病监测, 2021, 36(9): 938-942. doi: 10.3784/jbjc.202103160128
Chen Shaojin, Wang Licheng, Zhu Xiong, Fu Xiaoying, Chen Hai, Liang Lijuan, Han Limei. Colonization of Burkholderia pseudomallei in cucumber and lettuce seedlings[J]. Disease Surveillance, 2021, 36(9): 938-942. doi: 10.3784/jbjc.202103160128
Citation: Chen Shaojin, Wang Licheng, Zhu Xiong, Fu Xiaoying, Chen Hai, Liang Lijuan, Han Limei. Colonization of Burkholderia pseudomallei in cucumber and lettuce seedlings[J]. Disease Surveillance, 2021, 36(9): 938-942. doi: 10.3784/jbjc.202103160128

类鼻疽伯克霍尔德菌在青瓜和生菜幼苗中的定植情况研究

doi: 10.3784/jbjc.202103160128
基金项目: 海南省重点研发计划项目(No. ZDYF2019149);三亚市医疗卫生科技创新项目(No. 2019YW20)
详细信息
    作者简介:

    陈少金,女,海南省三亚市人,本科,主管技师,主要从事微生物检验工作,Email:290322561@qq.com

    通讯作者:

    韩丽梅,Tel:0898–88021159,Email:403732838@qq.com

  • 中图分类号: R211;R378

Colonization of Burkholderia pseudomallei in cucumber and lettuce seedlings

Funds: This study was supported by the Key Scientific Research Project in Hainan Province (No. ZDYF2019149) and Science and Technology Innovation Project of Medical and Health of Sanya City (No. 2019YW20)
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  • 摘要:   目的  研究类鼻疽伯克霍尔德菌在青瓜、生菜幼苗中的定植情况。  方法  用海南省三亚市人民医院保存的类鼻疽伯克霍尔德菌BP47、BP56、BP66分别感染青瓜、生菜无菌幼苗根部和叶片,从被感染幼苗根部与叶片的研磨液中分离该菌。 采用菌落平板计数检测被感染第1、3、5、7天的幼苗叶片中类鼻疽伯克霍尔德菌的增殖情况,绘制增殖曲线。 通过透射电镜对幼苗叶片中的类鼻疽伯克霍尔德菌进行定位。  结果  从被感染的青瓜、生菜幼苗中分离到了类鼻疽伯克霍尔德菌,而且该菌可以在幼苗中增殖,菌量随着时间推移而增加。 通过透射电镜在青瓜、生菜幼苗叶片中也观察到了该菌定植。  结论  类鼻疽伯克霍尔德菌可以在一些生食类蔬菜(青瓜、生菜)幼苗中定植,为探究其新的感染方式提供了实验依据。
  • 图  1  感染幼苗中分离培养的类鼻疽伯克霍尔德菌

    注:在ASH选择性培养基上生长48 h

    Figure  1.  B. pseudomallei isolated from infected plantlets

    图  2  类鼻疽伯克霍尔德菌感染的青瓜、生菜幼苗的形态变化

    注:A1. 未感染的青瓜幼苗;A2. 感染3 d的青瓜幼苗;A3. 感染7 d的青瓜幼苗;B1. 未感染的生菜幼苗;B2. 感染3 d的生菜幼苗;B3. 感染7 d的生菜幼苗

    Figure  2.  Morphological changes of cucumber and lettuce seedlings infected by B. pseudomallei

    图  3  类鼻疽伯克霍尔德菌在青瓜、生菜幼苗中的增殖情况

    Figure  3.  Proliferation of B. pseudomallei in cucumber and lettuce seedlings

    图  4  类鼻疽伯克霍尔德菌在感染幼苗中的定位

    注:除特殊说明,箭头指示为类鼻疽伯克霍尔德菌;A. 未侵染入幼苗的类鼻疽伯克霍尔德菌;B. 青瓜幼苗中的类鼻疽伯克霍尔德菌;C. 生菜幼苗中的类鼻疽伯克霍尔德菌

    Figure  4.  Localization of B. pseudomallei in infected seedlings

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  • 收稿日期:  2021-03-16
  • 网络出版日期:  2021-09-16
  • 刊出日期:  2021-09-30

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