陈嘉, 石琦, 陈操, 董小平. 朊病毒感染细胞模型中趋化因子CXCL1变化特征研究[J]. 疾病监测, 2022, 37(2): 172-175. DOI: 10.3784/jbjc.202105280293
引用本文: 陈嘉, 石琦, 陈操, 董小平. 朊病毒感染细胞模型中趋化因子CXCL1变化特征研究[J]. 疾病监测, 2022, 37(2): 172-175. DOI: 10.3784/jbjc.202105280293
Chen Jia, Shi Qi, Chen Cao, Dong Xiaoping. Study on the changes of chemokine CXCL1 in prion-infected cell model[J]. Disease Surveillance, 2022, 37(2): 172-175. DOI: 10.3784/jbjc.202105280293
Citation: Chen Jia, Shi Qi, Chen Cao, Dong Xiaoping. Study on the changes of chemokine CXCL1 in prion-infected cell model[J]. Disease Surveillance, 2022, 37(2): 172-175. DOI: 10.3784/jbjc.202105280293

朊病毒感染细胞模型中趋化因子CXCL1变化特征研究

Study on the changes of chemokine CXCL1 in prion-infected cell model

  • 摘要:
      目的   探究CXCL1在朊病毒感染细胞模型SMB-S15中的变化特征,比较感染细胞内朊病毒含量变化对CXCL1水平变化的影响。
      方法  通过荧光定量PCR、蛋白免疫印迹和酶联免疫吸附试验(ELISA)对朊病毒感染细胞模型SMB-S15及其对照细胞SMB-PS、SMB-RES中CXCL1的转录及翻译水平进行分析。 利用ELISA对用白藜芦醇和3,4-二羟基苯亚甲基丙酮(DBL)分别处理不含朊病毒和含少量朊病毒的SMB-S15细胞的CXCL1蛋白水平进行分析。
      结果  在朊病毒感染细胞SMB-S15中,CXCL1的转录水平显著高于对照细胞SMB-PS和SMB-RES,差异有统计学意义。 在细胞裂解液和对应的培养基中,SMB-S15细胞的CXCL1蛋白表达水平均高于SMB-PS和SMB-RES细胞。 白藜芦醇处理不同时间的SMB-S15细胞和不同浓度DBL处理的SMB-S15细胞中CXCL1蛋白水平均呈下降趋势,具有时间和剂量依赖性。
      结论  朊病毒感染细胞模型中CXCL1含量明显升高,且与胞内朊病毒的含量呈正相关。

     

    Abstract:
      Objective  To explore the change characteristics of chemokine CXCL1 in prion-infected cell model SMB-S15, and compare the effect of change of prion contents on CXCL1 level in prion-infected cells.
      Methods  The transcription and translation levels of CXCL1 in SMB-PS, SMB-RES and SMB-S15 cells were analyzed by fluorescence quantitative PCR, western blot and enzyme-linked immunosorbent assay (ELISA). The protein levels of CXCL1 in SMB-S15 cells without prion and with little prion treated with resveratrol and 3, 4-dihydroxyphenylene methylene acetone respectively were analyzed by ELISA.
      Results  In prion-infected SMB-S15 cells, the transcription level of CXCL1 was significantly higher than that in SMB-PS and SMB-RES cells, showing statistical difference. The expression level of CXCL1 in SMB-S15 cells was higher than that in SMB-PS and SMB-RES cells in both cell lysate and corresponding medium. The protein level of CXCL1 in SMB-S15 cells treated with resveratrol at different times and treated with 3, 4-dihydroxyphenylene methylene acetone at different concentrations all showed decreasing trendsPin a time and dose dependent manner.
      Conclusion  The level of CXCL1 significantly increased in prion-infected cells, and was positively correlated with the level of intracellular prions.

     

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