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沙比热木·托合塔木, 朱曦, 孔翔羽, 章青, 李静欣, 段招军, 马合木提 , 靳淼. 2017－2019年新疆维吾尔自治区乌鲁木齐市5岁以下腹泻住院患儿诺如病毒分子流行病学特征[J]. 疾病监测, 2021, 36(8): 789-793. DOI: 10.3784/jbjc.202106070322
引用本文: 沙比热木·托合塔木, 朱曦, 孔翔羽, 章青, 李静欣, 段招军, 马合木提 , 靳淼. 2017－2019年新疆维吾尔自治区乌鲁木齐市5岁以下腹泻住院患儿诺如病毒分子流行病学特征[J]. 疾病监测, 2021, 36(8): 789-793. DOI: 10.3784/jbjc.202106070322
shu
Shabiremu· Tuohetamu, Zhu Xi, Kong Xiangyu, Zhang Qing, Li Jingxin, Duan Zhaojun, Mahemuti  , Jin Miao. Molecular epidemiology of norovirus infection in hospitalized diarrheal children aged <5 years in Urumqi, 2017–2019[J]. Disease Surveillance, 2021, 36(8): 789-793. DOI: 10.3784/jbjc.202106070322
Citation: Shabiremu· Tuohetamu, Zhu Xi, Kong Xiangyu, Zhang Qing, Li Jingxin, Duan Zhaojun, Mahemuti  , Jin Miao. Molecular epidemiology of norovirus infection in hospitalized diarrheal children aged <5 years in Urumqi, 2017–2019[J]. Disease Surveillance, 2021, 36(8): 789-793. DOI: 10.3784/jbjc.202106070322
shu

2017－2019年新疆维吾尔自治区乌鲁木齐市5岁以下腹泻住院患儿诺如病毒分子流行病学特征

Molecular epidemiology of norovirus infection in hospitalized diarrheal children aged <5 years in Urumqi, 2017–2019

    摘要
  • 摘要:
      目的  了解2017 — 2019年新疆维吾尔自治区（新疆）乌鲁木齐市5岁以下腹泻住院患儿诺如病毒的分子流行病学特征。
      方法  收集2017 — 2019年乌鲁木齐市5岁以下腹泻住院患儿粪便标本和相应临床资料，应用实时荧光反转录聚合酶链式反应（real time RT-PCR）进行诺如病毒核酸检测，阳性样本通过RT-PCR扩增、测序和序列分析，并结合临床资料分析流行病学特征。
      结果  腹泻住院患儿粪便标本核酸检测阳性率为25.20%（246/976），不同年份阳性率差异有统计学意义（χ2＝40.724，P<0.0001）；0.5～1岁年龄组最高（30.61%，101/330），不同年龄组阳性率差异无统计学意义。 110份样本获得ORF1/ORF2重叠区序列，包括7个基因型，其中GⅡ.4 Sydney［P31］为流行优势株，占59.09%（65/110），其次是GⅡ.3［P12］（18.18%，20/110）和GⅡ.2［P16］（17.27%，19/110），其他型别包括GⅡ.6［P7］（2.72%，3/110）、GⅡ.4 Sydney［P16］（0.91%，1/110）、GⅡ.1 ［P16］（0.91%，1/110）和GⅠ.4［P4］（0.91%，1/110）。GⅡ.2［P16］自2017年第二季度出现，在2019年第三季度成为流行优势株。
      结论  在新疆地区诺如病毒是引起5岁以下儿童腹泻的主要病原之一，GⅡ.4 Sydney［P31］、GⅡ.3［P12］和GⅡ.2［P16］是该地区流行的主要基因型。

     

    Abstract:
      Objective  To understand the molecular epidemiological characteristics of norovirus infection in hospitalized diarrheal children under 5 years old in Urumqi, Xinjiang Uygur Autonomous Region, from 2017 to 2019.
      Methods  Stool samples and epidemiological data were collected from 976 sick children under 5 years old hospitalized in Urumqi Hospital due to acute diarrhea from 2017 to 2019, and Real-time fluorescence quantitative-polymerase chain reaction was performed for norovirus detection. The positive samples were amplified by reverse transcription-polymerase chain reaction and sequenced. The epidemiological characteristics were analyzed combined with clinical data.
      Results  The detection rate of norovirus nucleic acid was 25.20% (246/976), the differences in positive rate among different years were significant (χ2＝40.724, P<0.0001). The detection rate of norovirus nucleic acid was highest in 0.5–1 years old group (30.61%, 101/330), and there were no significant differences among different age groups. The sequences in the ORF1/ORF2 overlap were obtained from 110 strains, including 7 genotypes. GⅡ. 4 Sydney ［P31］ was predominant, accounting for 59.09% (65/110), followed by GⅡ. 3 ［P12］ (18.18%, 20/110) and GⅡ. 2 ［P16］ (17.27%, 19/110), and other genotypes included GⅡ. 6 ［P7］ (2.72%, 3/110), GⅡ. 4 Sydney ［P16］ (0.91%, 1/110), GⅡ. 1 ［P16］ (0.91%, 1/110) and GⅠ. 4 ［P4］(0.91%, 1/110). GⅡ. 2 ［P16］ emerged in the second quarter of 2017 and became predominant in the third quarter of 2019.
      Conclusion  Norovirus is one of the main pathogens causing diarrhea in children under 5 years old in Xinjiang. GⅡ. 4 Sydney ［P31］, GⅡ. 3 ［P12］ and GⅡ. 2 ［P16］ are the main genotypes of norovirus in Xinjiang.

     

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