2017-2019年新疆维吾尔自治区乌鲁木齐市5岁以下腹泻住院患儿诺如病毒分子流行病学特征

沙比热木·托合塔木 朱曦 孔翔羽 章青 李静欣 段招军 马合木提  靳淼

沙比热木·托合塔木, 朱曦, 孔翔羽, 章青, 李静欣, 段招军, 马合木提 , 靳淼. 2017-2019年新疆维吾尔自治区乌鲁木齐市5岁以下腹泻住院患儿诺如病毒分子流行病学特征[J]. 疾病监测, 2021, 36(8): 789-793. doi: 10.3784/jbjc.202106070322
引用本文: 沙比热木·托合塔木, 朱曦, 孔翔羽, 章青, 李静欣, 段招军, 马合木提 , 靳淼. 2017-2019年新疆维吾尔自治区乌鲁木齐市5岁以下腹泻住院患儿诺如病毒分子流行病学特征[J]. 疾病监测, 2021, 36(8): 789-793. doi: 10.3784/jbjc.202106070322
Shabiremu· Tuohetamu, Zhu Xi, Kong Xiangyu, Zhang Qing, Li Jingxin, Duan Zhaojun, Mahemuti  , Jin Miao. Molecular epidemiology of norovirus infection in hospitalized diarrheal children aged <5 years in Urumqi, 2017–2019[J]. Disease Surveillance, 2021, 36(8): 789-793. doi: 10.3784/jbjc.202106070322
Citation: Shabiremu· Tuohetamu, Zhu Xi, Kong Xiangyu, Zhang Qing, Li Jingxin, Duan Zhaojun, Mahemuti  , Jin Miao. Molecular epidemiology of norovirus infection in hospitalized diarrheal children aged <5 years in Urumqi, 2017–2019[J]. Disease Surveillance, 2021, 36(8): 789-793. doi: 10.3784/jbjc.202106070322

2017-2019年新疆维吾尔自治区乌鲁木齐市5岁以下腹泻住院患儿诺如病毒分子流行病学特征

doi: 10.3784/jbjc.202106070322
基金项目: 新疆少数民族科技人才特殊培养计划(No. 2017D03008)
详细信息
    作者简介:

    沙比热木·托合塔木,女,新疆维吾尔自治区伊宁县人,研究生、主任医师,主要从事传染病预防、控制与检测,Email:sabire@163.com

    朱曦,女,湖北省荆州市人,硕士,主要从事全国诺如病毒暴发和散发监测及毒株变异规律研究,Email:656706711@qq.com

    通讯作者:

    靳淼,Tel:010–58900180,Email:jinmiao@ivdc.chinacdc.cn

  • 沙比热木·托合塔木和朱曦同为第一作者
  • 中图分类号: R211; R373.2

Molecular epidemiology of norovirus infection in hospitalized diarrheal children aged <5 years in Urumqi, 2017–2019

Funds: This study was supported by the Special Project of Scientific Research Talent Training in Minorities (No. 2017D03008)
  • 摘要:   目的  了解2017 — 2019年新疆维吾尔自治区(新疆)乌鲁木齐市5岁以下腹泻住院患儿诺如病毒的分子流行病学特征。  方法  收集2017 — 2019年乌鲁木齐市5岁以下腹泻住院患儿粪便标本和相应临床资料,应用实时荧光反转录聚合酶链式反应(real time RT-PCR)进行诺如病毒核酸检测,阳性样本通过RT-PCR扩增、测序和序列分析,并结合临床资料分析流行病学特征。  结果  腹泻住院患儿粪便标本核酸检测阳性率为25.20%(246/976),不同年份阳性率差异有统计学意义(χ2=40.724,P<0.0001);0.5~1岁年龄组最高(30.61%,101/330),不同年龄组阳性率差异无统计学意义。 110份样本获得ORF1/ORF2重叠区序列,包括7个基因型,其中GⅡ.4 Sydney[P31]为流行优势株,占59.09%(65/110),其次是GⅡ.3[P12](18.18%,20/110)和GⅡ.2[P16](17.27%,19/110),其他型别包括GⅡ.6[P7](2.72%,3/110)、GⅡ.4 Sydney[P16](0.91%,1/110)、GⅡ.1 [P16](0.91%,1/110)和GⅠ.4[P4](0.91%,1/110)。GⅡ.2[P16]自2017年第二季度出现,在2019年第三季度成为流行优势株。  结论  在新疆地区诺如病毒是引起5岁以下儿童腹泻的主要病原之一,GⅡ.4 Sydney[P31]、GⅡ.3[P12]和GⅡ.2[P16]是该地区流行的主要基因型。
    1)  沙比热木·托合塔木和朱曦同为第一作者
  • 图  1  诺如病毒核酸检测阳性季节分布

    Figure  1.  Seasonal distribution of positive detection of norovirus nucleic acid

    图  2  腹泻住院儿童粪便标本诺如病毒基因型分布

    Figure  2.  Genotype distribution of norovirus detected in stool samples of diarrheal children

    图  3  ORF1/ORF2重叠区系统进化分析

    注:▲. 本研究毒株

    Figure  3.  Phylogenetic tree of ORF1/ORF2 overlap(about 570 nt)of strains in this study

    表  1  腹泻住院儿童诺如病毒腹泻感染流行病学特征

    Table  1.   Epidemiological characteristics of norovirus infection in hospitalized children due to diarrhea

      特征病例数(例)诺如病毒阳性数(例)阳性率(%)χ2P
    性别男性59314724.79 0.139 0.710
    女性3839925.85
    年龄组(岁)≤0.51472819.04 9.953 0.077
    0.5~33010130.61
    1~3368625.59
    2~802126.25
    3~34720.59
    4~19315.79
    年份201729511438.6440.724<0.0001
    20183326720.18
    20193496518.62
    季节春季(3—5月)1895629.6335.044<0.0001
    夏季(6—8月)30710835.18
    秋季(9—11月)2544216.53
    冬季(12月—次年2月)2264017.69
      合计97624625.20
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  • 收稿日期:  2021-06-07
  • 网络出版日期:  2021-08-16
  • 刊出日期:  2021-08-31

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