轮状病毒G9P [8] -E2基因型重配株在我国的流行趋势分析

王萌璇 马鑫 魏宇航 彭蕊 毛彤瑶 王明雯 范佳欣 李金松 李丹地

王萌璇, 马鑫, 魏宇航, 彭蕊, 毛彤瑶, 王明雯, 范佳欣, 李金松, 李丹地. 轮状病毒G9P [8] -E2基因型重配株在我国的流行趋势分析[J]. 疾病监测, 2021, 36(8): 780-784. doi: 10.3784/jbjc.202106210350
引用本文: 王萌璇, 马鑫, 魏宇航, 彭蕊, 毛彤瑶, 王明雯, 范佳欣, 李金松, 李丹地. 轮状病毒G9P [8] -E2基因型重配株在我国的流行趋势分析[J]. 疾病监测, 2021, 36(8): 780-784. doi: 10.3784/jbjc.202106210350
Wang Mengxuan, Ma Xin, Wei Yuhang, Peng Rui, Mao Tongyao, Wang Mingwen, Fan Jiaxin, Li Jinsong, Li Dandi. Spread trend analysis on reassortant of rotavirus G9P[8]-E2 in China[J]. Disease Surveillance, 2021, 36(8): 780-784. doi: 10.3784/jbjc.202106210350
Citation: Wang Mengxuan, Ma Xin, Wei Yuhang, Peng Rui, Mao Tongyao, Wang Mingwen, Fan Jiaxin, Li Jinsong, Li Dandi. Spread trend analysis on reassortant of rotavirus G9P[8]-E2 in China[J]. Disease Surveillance, 2021, 36(8): 780-784. doi: 10.3784/jbjc.202106210350

轮状病毒G9P [8] -E2基因型重配株在我国的流行趋势分析

doi: 10.3784/jbjc.202106210350
基金项目: 国家科技重大专项(No. 2018ZX10711–001,No. 2018ZX10201002);国家重点研发计划(No. 2018YFC1200602);国家自然科学基金(No. 21934005,No. 81601813)
详细信息
    作者简介:

    王萌璇,女,陕西省咸阳市人,学士,实习研究员,主要从事轮状病毒相关研究,Email:920504673@qq.com

    马鑫,女,吉林省通化市人,硕士,主管技师,主要从事生化检验工作,Email:bing-yu-2008@163.com

    通讯作者:

    李丹地,Tel:010–58900899,Email:dandili@126.com

  • 中图分类号: R211; R373.2

Spread trend analysis on reassortant of rotavirus G9P[8]-E2 in China

Funds: This study were supported by the National Science and Technology Major Project (No. 2018ZX10711–001, No. 2018ZX10201002), the National Key Research and Development Program of China (No. 2018YFC1200602), and the National Natural Science Foundation of China (No. 21934005, No. 81601813)
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  • 摘要:   目的  研究我国轮状病毒(RV)流行毒株G9P[8]基因型的全序列分子特征及进化规律。  方法  采用反转录–聚合酶链式反应(RT-PCR)方法,对2018年收集的64份(广东省深圳市和吉林省长春市各32份)G9P[8]基因型RV阳性标本进行扩增。  结果  成功获取54份(深圳市30份, 长春市24份)G9P[8]基因型A组轮状病毒(RVA)标本的全基因组节段。序列分析表明,54份G9P[8]基因型RVA中, 28份为Wa-like G9P[8]-E1株,26份为非结构蛋白NSP4基因节段重配的G9P[8]-E2株,G9P[8]-E2株在深圳和长春市阳性样本中分别占60.0%、33.3%。  结论  2018年深圳和长春市流行的RVA中G9P[8]-E2重配株已经成为优势株,且在深圳市的优势程度大于在长春市的,推测该基因型重配株RVA可能已在我国广泛传播。
  • 图  1  我国广东省深圳市、吉林省长春市G9P[8]基因型RVA NSP4基因核苷酸系统进化分析

    注:●. 我国广东省深圳市G9P[8]基因型RVA毒株;▲. 我国吉林省长春市G9P[8]基因型RVA毒株

    Figure  1.  Phylogenetic tree based on NSP4 gene of RVA G9P[8] strains in Shenzhen of Guangdong province and Changchun of Jilin province, China

    表  1  我国广东省深圳市、吉林省长春市G9P[8]基因型RVA全基因组分型

    Table  1.   Full genome type of G9P[8] strains of RVA in Shenzhen of Guangdong province and Changchun of Jilin province, China

    样本来源地样本数(份)VP7VP4VP6VP1VP2VP3NSP1NSP2NSP3NSP4NSP5
    广东省深圳市12G9P[8]I1R1C1M1A1N1T1E1H1
    广东省深圳市18G9P[8]I1R1C1M1A1N1T1E2H1
    吉林省长春市16G9P[8]I1R1C1M1A1N1T1E1H1
    吉林省长春市 8G9P[8]I1R1C1M1A1N1T1E2H1
    下载: 导出CSV

    表  2  我国广东省深圳市、吉林省长春市G9P[8]基因型RVA的NSP4基因同源性分析

    Table  2.   Genome homology analyzing of NSP4 of RVA G9P[8] strains in Shenzhen of Guangdong province and Changchun of Jilin province, China

       样本来源地样本数(份)NSP4基因型NSP4基因同源性(%)
    中国广东省深圳市 12E1 99.0~99.9
    中国广东省深圳市18E299.3~100.0
    中国吉林省长春市16E199.3~99.9
    中国吉林省长春市8E299.3~99.9
    中国广东省深圳市、吉林省长春市28E198.5~99.9
    中国广东省深圳市、吉林省长春市26E299.0~100.0
    中国广东省深圳市、吉林省长春市+日本东京市32E297.2~100.0
    中国广东省深圳市、吉林省长春市、辽宁省锦州市29E297.2~100.0
    中国广东省深圳市、吉林省长春市、湖北省武汉市29E297.3~100.0
    下载: 导出CSV
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  • 收稿日期:  2021-06-21
  • 网络出版日期:  2021-08-21
  • 刊出日期:  2021-08-31

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