2019-2020年浙江省湖州市副溶血弧菌临床分离株特征分析

徐德顺 严伟 纪蕾

徐德顺, 严伟, 纪蕾. 2019-2020年浙江省湖州市副溶血弧菌临床分离株特征分析[J]. 疾病监测, 2022, 37(5): 613-617. doi: 10.3784/jbjc.202107050384
引用本文: 徐德顺, 严伟, 纪蕾. 2019-2020年浙江省湖州市副溶血弧菌临床分离株特征分析[J]. 疾病监测, 2022, 37(5): 613-617. doi: 10.3784/jbjc.202107050384
Xu Deshun, Yan Wei, Ji Lei. Characterization of clinical isolates of Vibrio parahaemolyticus in HuZhou, Zhejiang, 2019−2020[J]. Disease Surveillance, 2022, 37(5): 613-617. doi: 10.3784/jbjc.202107050384
Citation: Xu Deshun, Yan Wei, Ji Lei. Characterization of clinical isolates of Vibrio parahaemolyticus in HuZhou, Zhejiang, 2019−2020[J]. Disease Surveillance, 2022, 37(5): 613-617. doi: 10.3784/jbjc.202107050384

2019-2020年浙江省湖州市副溶血弧菌临床分离株特征分析

doi: 10.3784/jbjc.202107050384
详细信息
    作者简介:

    徐德顺,男,安徽省舒城县人,主任技师,主要从事微生物检验工作

    通讯作者:

    徐德顺,Tel:0572–2760125,Email:xds666092@163.com

  • 中图分类号: R211; R378.3

Characterization of clinical isolates of Vibrio parahaemolyticus in HuZhou, Zhejiang, 2019−2020

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  • 摘要:   目的  了解2019—2020年浙江省湖州市腹泻患者副溶血弧菌分离株的特征。  方法  对2019—2020年分离自腹泻患者的109株副溶血弧菌进行血清学分型,采用荧光PCR方法检测其毒力基因,采用微量肉汤稀释法检测其耐药性,并利用脉冲场凝胶电泳(PFGE)对其进行分子分型。  结果  109株分离株的优势血清型为O3∶K6(72株)。 所有分离株均携带tlh基因,仅2株菌携带trh基因。 108株菌产生72种PFGE带型,不同带型的相似系数为19.10%~100%。 分离株对磺胺甲恶唑的耐药率最高(63.30%),其次为氨苄西林(49.54%)。  结论  加强腹泻患者副溶血弧菌分离株的持续监测有助于开展副溶血弧菌引起的食源性疾病的风险评估,为其引起的肠道疾病防控及临床治疗用药提供参考。
  • 图  1  109株副溶血弧菌的血清学分型

    Figure  1.  Serological typing of 109 V. parahaemolyticus strains

    图  2  108株副溶血弧菌的脉冲场凝胶电泳图谱聚类分析

    Figure  2.  Results of PFGE molecular typing of 108 V. parahaemolyticus strains

    表  1  各哨点医院腹泻患者标本副溶血弧菌分离情况

    Table  1.   Isolation of Vibrio parahaemolyticus in sentinel hospitals

     哨点医院2019年2020年
    标本数
    (份)
    分离
    菌株数
    (株)
    标本
    阳性率
    (%)
    标本数
    (份)
    分离
    菌株数
    (株)
    标本
    阳性率
    (%)
    安吉县人民医院 257 2710.51 255155.88
    德清县人民医院 415 10 2.41 404215.20
    南浔区人民医院 289 12 4.15 279 51.79
    吴兴区人民医院 197 18 9.14 187 73.74
    长兴县人民医院 460 5712.39 400184.50
    湖州市第一人民医院 533 22 4.13 635152.36
      合计2 151146 6.792 160813.75
    下载: 导出CSV

    表  2  109株副溶血弧菌的毒力基因携带情况

    Table  2.   Virulence gene carriages of 109 V. parahaemolyticus strains

    血清型菌株数(株)tlhtdhtrh
    O1∶Kut 2+
    O3∶K669++
    2+
    O3∶Kut12++
    2++
    O4∶K8 4++
    O4∶Kut15++
    2+
    O5∶Kut 1++
     注:+. 阳性;–. 阴性
    下载: 导出CSV

    表  3  109株副溶血弧菌的药物敏感性

    Table  3.   Susceptibilities of 109 V. parahaemolyticus strains to different antibiotics

    抗菌药物敏感中介耐药
    菌株数(株)敏感率(%)菌株数(株)耐药率(%)菌株数(株)耐药率(%)
    氨苄西林 4743.1287.345449.54
    头孢西丁10798.1721.83 0 0.00
    阿奇霉素10495.4154.59 0 0.00
    磺胺甲恶唑 4036.7000.006963.30
    复方新诺明10899.0810.92 0 0.00
    下载: 导出CSV
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  • 收稿日期:  2021-07-05
  • 网络出版日期:  2022-02-09
  • 刊出日期:  2022-05-31

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