董银苹, 王伟, 江涛, 徐进, 李凤琴. 引起我国首例婴儿肉毒中毒丁酸梭菌全基因组序列分析[J]. 疾病监测, 2022, 37(1): 38-44. DOI: 10.3784/jbjc.202108040431
引用本文: 董银苹, 王伟, 江涛, 徐进, 李凤琴. 引起我国首例婴儿肉毒中毒丁酸梭菌全基因组序列分析[J]. 疾病监测, 2022, 37(1): 38-44. DOI: 10.3784/jbjc.202108040431
Dong Yinping, Wang Wei, Jiang Tao, Xu Jin, Li Fengqin. Whole genome sequencing of Clostridium butyricum that caused the first infant botulism in China[J]. Disease Surveillance, 2022, 37(1): 38-44. DOI: 10.3784/jbjc.202108040431
Citation: Dong Yinping, Wang Wei, Jiang Tao, Xu Jin, Li Fengqin. Whole genome sequencing of Clostridium butyricum that caused the first infant botulism in China[J]. Disease Surveillance, 2022, 37(1): 38-44. DOI: 10.3784/jbjc.202108040431

引起我国首例婴儿肉毒中毒丁酸梭菌全基因组序列分析

Whole genome sequencing of Clostridium butyricum that caused the first infant botulism in China

  • 摘要:
      目的  对我国首次分离自婴儿肉毒中毒病例粪便样品的1株产E型肉毒毒素的丁酸梭菌进行全基因组测序,解析菌株产毒的遗传学特性。
      方法  对从婴儿肉毒中毒病例粪便样品中分离的产E型肉毒毒素丁酸梭菌分离株TJ-S提取基因组DNA,利用Pacific Biosciences RSⅡ 平台对菌株进行全基因组测序,并进行序列分析。
      结果  TJ-S基因组由一个染色体和一个质粒组成,大小分别为3 949 987 bp和747 151 bp。 GC含量分别为28.8%和28.2%。 染色体上携带产肉毒毒素的bont/e基因,该基因位于典型orfX基因簇内,且在基因簇上游存在3个转座酶编码基因。 遗传进化分析发现,菌株TJ-S在亲缘关系上与引起英国婴儿肉毒中毒的2株产E型肉毒毒素的丁酸梭菌分离株最为接近。
      结论  丁酸梭菌携带的产E型肉毒毒素基因位于稳定的orfX基因簇内,可引起人类肉毒中毒。 本报道是我国第一次对产E型肉毒毒素丁酸梭菌进行全基因组学分析。

     

    Abstract:
      Objective  To analyze the data obtained from a whole genome sequence of type E botulinum neurotoxin producing Clostridium butyricum TJ-S,which was isolated from stool sample of an infant botulism case for the first time in China.
      Methods  Genomic DNA was extracted from TJ-S isolate, the whole genome sequencing was carried out using Pacific Biosciences RS Ⅱ platform and analyzed.
      Results  One independent closed circle chromosome and one plasmid were found in TJ-S isolate. The lengths of the chromosome and plasmid were 3 949 987 bp and 747 151 bp, GC contents was 28.2% for chromosome and 28.2% for plasmid. The bont/e gene was found in the typical orfX cluster that located on chromosome, and there were three transposase encoding genes on upstream of the cluster. The phylogenetic analysis showed that TJ-S strain and two type E-producing C. butyricum stains which were isolated from infant botulism cases in the United Kingdom were in the same clade.
      Conclusion  The bont/e gene was located in the stable classic orfX cluster of C. butyricum, and it was the main pathogenic gene causing botulism in human. This is the first report of genomic data mining and analysis of type E botulinum neurotoxin producing C. butyricum in China.

     

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