江苏省东台地区家畜非O157产志贺毒素大肠埃希菌耐药性及多位点序列分型分析

韩悦 韩影 刘敏琪 董晨 金辉

韩悦, 韩影, 刘敏琪, 董晨, 金辉. 江苏省东台地区家畜非O157产志贺毒素大肠埃希菌耐药性及多位点序列分型分析[J]. 疾病监测, 2022, 37(5): 661-667. doi: 10.3784/jbjc.202112060625
引用本文: 韩悦, 韩影, 刘敏琪, 董晨, 金辉. 江苏省东台地区家畜非O157产志贺毒素大肠埃希菌耐药性及多位点序列分型分析[J]. 疾病监测, 2022, 37(5): 661-667. doi: 10.3784/jbjc.202112060625
Han Yue, Han Ying, Liu Minqi, Dong Chen, Jin Hui. Antibiotic resistance and multi-locus sequence typing of non-O157 Shiga toxin-producing Escherichia coli from livestock in Dongtai, Jiangsu[J]. Disease Surveillance, 2022, 37(5): 661-667. doi: 10.3784/jbjc.202112060625
Citation: Han Yue, Han Ying, Liu Minqi, Dong Chen, Jin Hui. Antibiotic resistance and multi-locus sequence typing of non-O157 Shiga toxin-producing Escherichia coli from livestock in Dongtai, Jiangsu[J]. Disease Surveillance, 2022, 37(5): 661-667. doi: 10.3784/jbjc.202112060625

江苏省东台地区家畜非O157产志贺毒素大肠埃希菌耐药性及多位点序列分型分析

doi: 10.3784/jbjc.202112060625
基金项目: 国家自然科学基金(No. 81573258)
详细信息
    作者简介:

    韩悦,女,江苏省扬州市人,硕士研究生,主要从事疾病预防与控制,Email:hanhy68@163.com

    通讯作者:

    董晨,Tel:025–83759339,Email:dongchen_jscdc@163.com

    金辉,Tel:025–83272572,Email:jinhui_hld@163.com

  • 中图分类号: R211; R378

Antibiotic resistance and multi-locus sequence typing of non-O157 Shiga toxin-producing Escherichia coli from livestock in Dongtai, Jiangsu

Funds: This study was supported by the National Natural Science Foundation of China (No. 81573258)
More Information
  • 摘要:   目的  了解江苏省东台地区家畜来源非O157产志贺毒素大肠埃希菌(STEC)抗生素耐药表型、耐药基因,以及多位点序列分型(MLST)情况。  方法  于2019年5月,江苏省东台市采集家畜粪便样本301份(羊粪便231份,牛粪便70份),分离非O157 STEC菌株。 采用改良微量肉汤法测定菌株对21种抗生素最小抑菌浓度(MIC);通过全基因组测序技术对O∶H血清型、耐药基因和多位点序列型别进行预测。  结果  在68株非O157 STEC中,有32株对至少1种药物耐药。 其中,STEC对四环素耐药率最高(42.6%),其次分别对阿奇霉素(36.8%)、复方新诺明(35.3%)、链霉素(32.3%)、氯霉素(30.9%)、环丙沙星(29.4%)等9种抗生素耐药;共识别出25种耐药基因,其中,氨基糖苷类耐药基因和叶酸途径抑制剂类耐药基因携带率最高(44.1%),其次为四环素类耐药基因tet(A)(42.6%);MLST将分离株分为13种ST型,其中ST43(19.1%)和ST155(16.2%)比例较高。 最小生成树显示,具有相同血清型的STEC菌株多聚集在一起。 STEC中4种ST型别(ST25、ST40、ST43、ST675)分别与引起肠溶血性尿毒综合征相关的肠出血性大肠埃希菌(HUSEC)聚类成簇。  结论  江苏东台地区家畜中非O157 STEC耐药形势复杂,且存在多重耐药现象。 此外,该地区STEC菌株对人群存在潜在威胁,家畜作为非O157 STEC宿主应引起重视。
  • 图  1  非O157 STEC耐药基因分布热图

    Figure  1.  Distribution of antibiotic resistance genes of non-O157 STEC strains

    图  2  非O157 STEC的ST型别及其与HUSEC遗传进化关系

    注:每个圆圈代表一种ST型别,圆圈大小代表菌株数量多少,圆圈间连线上的数字代表不同ST型别相差的等位基因数,圆圈间的粗细实线分别代表ST型别相差1-3个等位基因,虚线表示代表ST型别之间相差4个及以上等位基因差异。不同圆圈颜色表示不同STEC宿主来源

    Figure  2.  STs of non-O157 STEC strains and the phylogenetic relationships between non-O157 STEC strains and HUSEC

    表  1  68株非O157 STEC菌株血清型、毒力基因及ST型别分布

    Table  1.   Distribution of serotypes, virulence genes and sequence types (STs) in 68 non-O157 STEC strains

     血清型菌株数(株)菌株来源毒力基因ST型别
    O6∶H1013stx143
    O155∶H2110stx2683
    O21∶H257stx1155
    O22∶H87stx111997
    O66∶H457stx2971
    O38∶H266stx110
    OX18∶H216stx140
    O8∶H212stx1155
    O43∶H22stx2937
    O66∶H212stx1155
    O76∶H192stx1675
    O5∶H191stx1+stx2447
    O128∶H21stx225
    O150∶H81stx1906
    O182∶H251stx1+eae300
    下载: 导出CSV

    表  2  68株非O157 STEC药敏试验结果

    Table  2.   Antibiotic susceptibility test results of 68 non-O157 STEC strains

     抗菌药物菌株耐药分布
    敏感(S)中介(I)耐药(R)
    四环素39(57.4) 0(0.0)29(42.6)
    阿奇霉素43(63.2) 0(0.0)25(36.8)
    复方新诺明44(64.7) 0(0.0)24(35.3)
    链霉素1(1.5) 45(66.2)22(32.3)
    氯霉素47(69.1) 0(0.0)21(30.9)
    环丙沙星43(63.2) 5(7.4)20(29.4)
    氨苄西林56(82.4) 0(0.0)12(17.6)
    萘啶酸58(85.3) 0(0.0)10(14.7)
    氨曲南58(85.3) 1(1.5) 9(13.2)
    头孢噻肟59(86.8) 0(0.0) 9(13.2)
    多黏菌素E0(0.0) 68(100) 0(0.0)
    头孢他啶65(95.6) 3(4.4)0(0.0)
    氨苄西林–舒巴坦66(97.1) 2(2.9)0(0.0)
    厄他培南68(100.0)0(0.0)0(0.0)
    美罗培南68(100.0)0(0.0)0(0.0)
    亚胺培南68(100.0)0(0.0)0(0.0)
    头孢他啶–阿维巴坦68(100.0)0(0.0)0(0.0)
    头孢西丁68(100.0)0(0.0)0(0.0)
    替加环素68(100.0)0(0.0)0(0.0)
    阿米卡星68(100.0)0(0.0)0(0.0)
    呋喃妥因68(100.0)0(0.0)0(0.0)
      注:括号外数据为菌株数,括号内数据为百分比(%)
    下载: 导出CSV

    表  3  非O157 STEC菌株多重耐药组合

    Table  3.   Combination of MDR in non-O157 STEC strains

       抗菌药物组合耐药菌株数(株)
    CHL+SXT+CTX+TET+CIP+AZM+STR+AMP+ATM
    8
    CHL+TET+STR4
    SXT+TET+CIP+NAL+AZM+STR4
    CHL+SXT+TET+AZM3
    SXT+TET+CIP+NAL+AZM3
    CHL+SXT+TET+CIP+AZM+STR+AMP2
    CHL+SXT+CTX+TET+CIP+AZM+STR+AMP1
    CHL+SXT+TET+AZM+STR1
    CHL+SXT+TET+CIP+AZM1
    CHL+SXT+TET+CIP+AZM+STR+AMP+ATM1
    TET+NAL+STR1
    注:CHL. 氯霉素;SXT. 复方新诺明;CTX. 头孢噻肟;TET. 四环素;AZM. 阿奇霉素;STR. 链霉素;AMP. 氨苄西林;ATM. 氨曲南;CIP. 环丙沙星;NAL. 萘啶酸
    下载: 导出CSV

    表  4  非O157 STEC携带耐药基因和表型耐药关系

    Table  4.   Correlation between antibiotic resistance genes and antibiotic resistance phenotypes in non-O157 STEC strains

      药物类别耐药基因组合(菌株数)携带耐药基因菌株总数(株)表型耐药菌株总数(株)耐药基因携带率(%)
    氨基糖苷类aac(3)-IId+aph(3')-Ia+aph(3'')-Ib+aph(6)-Id(9)
    aadA1(7)
    aac(3)-IId+aac(6')-Ib-cr+aph(3'')-Ib+aph(6)-Id(6)
    aph(3'')-Ib+aph(6)-Id(5)
    aac(3)-IId+aac(6')-Ib-cr+aph(3'')-Ib+aph(6)-Id+aadA16(1)
    aac(3)-IId+aph(3'')-Ia+aph(6)-Id(1)
    aac(3)-IId(1)
    302244.1
    叶酸途径抑制剂sul2+sul3+dfrA14(10)
    sul1+dfrA1(7)
    sul2(5)
    sul1+dfrA27(4)
    sul1+sul2+dfrA27(3)
    sul1(1)
    302444.1
    四环素类tet(A)(29)292942.6
    喹诺酮类gyrA+qnrS1(9)
    qnrS1(8)
    aac(6')-Ib-cr+qnrB6(7)
    gyrA(3)
    qnrB6(1)
    282341.2
    大环内酯类mph(A)(25)252536.8
    氯霉素类floR(21)212130.9
    β-内酰胺类blaCTX-M-55(10)
    blaTEM-1B(2)
    121217.6
      注:字体加粗基因为耐药基因型和耐药表型不一致者
    下载: 导出CSV
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  • 收稿日期:  2021-12-06
  • 网络出版日期:  2022-02-21
  • 刊出日期:  2022-05-31

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